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NYU Langone Health

3 ARTICLES PUBLISHED IN JoVE

Cancer Research

3-D Cell Culture System for Studying Invasion and Evaluating Therapeutics in Bladder Cancer

Yin Wang¹, Mark L. Day², Diane M. Simeone³, Phillip L. Palmbos¹

¹Departments of Internal Medicine, Hematology/Oncology Division, Rogel Cancer Center, University of Michigan Medical Center, ²Department of Urology, Division of GU Oncology, Rogel Cancer Center, University of Michigan Medical Center, ³Departments of Surgery and Pathology, Perlmutter Cancer Center, NYU Langone Health

The processes governing bladder cancer invasion represent opportunities for biomarker and therapeutic development. Here we present a bladder cancer invasion model which incorporates 3-D culture of tumor spheroids, time-lapse imaging and confocal microscopy. This technique is useful for defining the features of the invasive process and for screening therapeutic agents.

Bioengineering

Bioinspired Soft Robot with Incorporated Microelectrodes

Ting Wang^1,2, Bianca Migliori^1,3, Beatrice Miccoli^1,4, Su Ryon Shin¹

¹Division of Engineering in Medicine, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, ²School of Medicine, Jiangsu University, ³Tech4Health and Neuroscience Institutes, NYU Langone Health, ⁴Department of Electronics and Telecommunication, Politecnico di Torino

A bioinspired scaffold is fabricated by a soft photolithography technique using mechanically robust and electrically conductive hydrogels. The micropatterned hydrogels provide directional cardiomyocyte cell alignment, resulting in a tailored direction of actuation. Flexible microelectrodes are also integrated into the scaffold to bring electrical controllability for a self-actuating cardiac tissue.

Biology

Visualizing Single-Stranded DNA Foci in the G1 Phase of the Cell Cycle

Qingyue Zhang^1,2, Marc A. Kerzhnerman^1,2, Nelson García-Vázquez^1,2,3, Gergely Rona^1,2,4,5

¹Department of Biochemistry and Molecular Pharmacology, NYU Grossman School of Medicine, ²The Laura and Isaac Perlmutter Cancer Center, NYU Langone Health, ³Department of Cell Biology, NYU Grossman School of Medicine, ⁴Howard Hughes Medical Institute, NYU Grossman School of Medicine, ⁵Institute of Enzymology, Centre of Excellence of the Hungarian Academy of Sciences, HUN-REN Research Centre for Natural Sciences

The following protocol presents the detection of single-stranded DNA foci in the G1 phase of the cell cycle utilizing cell cycle synchronization followed by RPA2 immunofluorescent staining.