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Univ. Grenoble Alpes, CNRS, CEA, IBS (Institut de Biologie Structurale)

1 ARTICLES PUBLISHED IN JoVE

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Biochemistry

Structure Solution of the Fluorescent Protein Cerulean Using MeshAndCollect
Stephanie Hutin *1, Gianluca Santoni *1, Ulrich Zander 2, Nicolas Foos 1, Sylvain Aumonier 1, Guillaume Gotthard 1, Antoine Royant 1,3, Christoph Mueller-Dieckmann 1, Gordon Leonard 1
1European Synchrotron Radiation Facility, Structural Biology Group, 2European Molecular Biology Laboratory, 3Univ. Grenoble Alpes, CNRS, CEA, IBS (Institut de Biologie Structurale)

We present the use of the MeshAndCollect protocol to obtain a complete diffraction data set, for use in subsequent structure determination, composed of partial diffraction data sets collected from many small crystals of the fluorescent protein Cerulean.

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