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HKUST Shenzhen Research Institute

1 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

Photobleaching Enables Super-resolution Imaging of the FtsZ Ring in the Cyanobacterium Prochlorococcus

Yuanchao Zhan¹, Yaxin Liu¹, Qinglu Zeng^1,2,3

¹Department of Ocean Science, Hong Kong University of Science and Technology, ²Division of Life Science, Hong Kong University of Science and Technology, ³HKUST Shenzhen Research Institute

Here we describe a photobleaching method to reduce the autofluorescence of cyanobacteria. After photobleaching, stochastic optical reconstruction microscopy is used to obtain three-dimensional super-resolution images of the cyanobacterial FtsZ ring.