A Semi-high-throughput Imaging Method and Data Visualization Toolkit to Analyze C. elegans Embryonic DevelopmentRenat N. Khaliullin 1,2,3, Jeffrey M. Hendel 1,2, Adina Gerson-Gurwitz 1,2, Shaohe Wang 1,4,5, Stacy D. Ochoa 1,6, Zhiling Zhao 1,7, Arshad Desai 1,2, Karen Oegema 1,2, Rebecca A. Green 1,2
1Ludwig Institute for Cancer Research, San Diego, 2Department of Cellular and Molecular Medicine, University of California, San Diego, 3Recursion Pharmaceuticals, 4Biomedical Sciences Graduate Program, University of California, San Diego, 5Cell Biology Section, National Institute of Dental and Craniofacial Research, National Institutes of Health, 6Department of Biology, San Diego State University, 7Developmental and Stem Cell Biology Graduate Program, University of California, San Francisco
This work describes a semi-high-throughput protocol that allows simultaneous 3D time-lapse imaging of embryogenesis in 80–100 C. elegans embryos in a single overnight run. Additionally, image processing and visualization tools are included to streamline data analysis. The combination of these methods with custom reporter strains enables detailed monitoring of embryogenesis.
