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Geisel School of Medicine & Dartmouth-Hitchcock Medical Center

3 ARTICLES PUBLISHED IN JoVE

Neuroscience

Measuring Progressive Neurological Disability in a Mouse Model of Multiple Sclerosis

Francesca Gilli¹, Darlene B. Royce¹, Andrew R. Pachner¹

¹Department of Neurology, Geisel School of Medicine at Dartmouth

An optimized testing protocol is presented in this paper for the Rotarod performance test, used for measuring progressive neurological disability in TMEV-infected mice.

Neuroscience

Quantitative Measurement of Intrathecally Synthesized Proteins in Mice

Francesca Gilli¹, Nora C. Welsh^1,2, Michael R. Linzey^1,2, Darlene B. Royce¹, Krista D. DiSano¹, Andrew R. Pachner¹

¹Department of Neurology, Geisel School of Medicine & Dartmouth-Hitchcock Medical Center, ²Program in Experimental and Molecular Medicine, Dartmouth College

Elevated spinal fluid protein levels can either be the result of diffusion of plasma protein across an altered blood-brain barrier or intrathecal synthesis. An optimized testing protocol is presented in this article that helps to discriminate both cases and provides quantitative measurements of intrathecally synthesized proteins.

Immunology and Infection

Isolating Central Nervous System Tissues and Associated Meninges for the Downstream Analysis of Immune cells

Krista D. DiSano¹, Michael R. Linzey^1,2, Nora C. Welsh^1,2, Joshua S. Meier¹, Andrew R. Pachner¹, Francesca Gilli¹

¹Department of Neurology, Geisel School of Medicine, Dartmouth-Hitchcock Medical Center, ²Program in Experimental and Molecular Medicine, Dartmouth College

This paper presents two optimized protocols for examining resident and peripherally derived immune cells within the central nervous system, including the brain, spinal cord, and meninges. Each of these protocols helps to ascertain the function and composition of the cells occupying these compartments under steady state and inflammatory conditions.