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UCSF Mission Bay

4 ARTICLES PUBLISHED IN JoVE

Biology

Visualizing Cytoplasmic Flow During Single-cell Wound Healing in Stentor coeruleus

Mark Slabodnick^1,2, Bram Prevo^1,3, Peter Gross^1,4, Janet Sheung^1,5, Wallace Marshall^1,2

¹Physiology Course, Marine Biological Laboratory, ²Department of Biochemistry & Biophysics, University of California San Francisco, ³Department of Physics and Astronomy, Vrije Universiteit Amsterdam, ⁴Max Planck Institute of Molecular Cell Biology and Genetics, ⁵Department of Physics, University of Illinois Urbana-Champaign

The giant ciliate Stentor coeruleus is a classical system for studying regeneration and wound healing in single cells. By imaging Stentor cells simultaneously at low and high magnification it is possible to measure cytoplasmic flows before, during, and after wounding.

Developmental Biology

Methods for the Study of Regeneration in Stentor

Athena Lin¹, Tatyana Makushok¹, Ulises Diaz¹, Wallace F. Marshall¹

¹Department of Biochemistry and Biophysics, University of California San Francisco

The giant ciliate, Stentor coeruleus, is an excellent system to study regeneration and wound healing. We present procedures for establishing Stentor cell cultures from single cells or cell fragments, inducing regeneration by cutting cells, chemically inducing the regeneration of membranellar band and oral apparatus, imaging, and analysis of cell regeneration.

Developmental Biology

Drosophila Embryo Preparation and Microinjection for Live Cell Microscopy Performed using an Automated High Content Analyzer

Ulises Diaz^1,2, Wallace Marshall², Blake Riggs¹

¹Department of Biology, San Francisco State University, ²Department of Biochemistry & Biophysics, UCSF Mission Bay

Presented here is a protocol to microinject and simultaneously image multiple Drosophila embryos during embryonic development using a plate-based, high content imager.

Biology

Studying Habituation in Stentor coeruleus

Deepa Rajan¹, Peter Chudinov¹, Wallace Marshall¹

¹Department of Biochemistry and Biophysics, University of California San Francisco

We introduce a method for quantifying Stentor habituation using a microcontroller board-linked apparatus that can deliver mechanical pulses at a specified force and frequency. We also include methods for assembling the apparatus and setting up the experiment in a way that minimizes external perturbations.