In Vitro Preparation of Actively Maturing Bovine Articular Cartilage Explants for X-Ray Phase Contrast ImagingCaroline Bissardon 1,2,3, Yadan Zhang 2, Hélène Rougé Labriet 1,4, Sébastien Berujon 5, Laurent Charlet 3, Ilyas M. Khan 2, Emmanuel Brun 1, Sylvain Bohic 1
1Inserm UA07, Rayonnement Synchrotron pour la Recherche Biomédicale (STROBE), 2Centre of Nanohealth, Swansea University Medical School, 3ISTerre, Université Grenoble Alpes, CS, Grenoble Cedex 9, 4Novitom, R&D Grenoble, 5ESRF, European Synchrotron Radiation Facility, CS, Grenoble Cedex 9
This protocol describes the preparation of in vitro bovine articular cartilage for imaging in high resolution with X-rays. These explants actively undergo postnatal maturation. We describe here the necessary steps from the biopsy to data analysis of 3D X-ray phase contrast imaging, passing through explant culture, tissue fixation and synchrotron preparation.
