Universitätsmedizin Essen, University Duisburg-Essen
2 ARTICLES PUBLISHED IN JoVE
Genetics
Measurement of BK-polyomavirus Non-Coding Control Region Driven Transcriptional Activity Via Flow Cytometry
Johannes Korth 1,2, Helene Sertznig 2, Siegfried Moyrer 2, Adrian Atilla Nicolas Doevelaar 2,3, Timm Henning Westhoff 3, Nina Babel 3, Oliver Witzke 4, Andreas Kribben 1, Ulf Dittmer 2, Marek Widera 2
1Department of Nephrology, University of Duisburg-Essen, University Hospital Essen, 2Institute for Virology, University of Duisburg-Essen, University Hospital Essen, 3Center for Translational Medicine, Medical Department I, Marien Hospital Herne, University Hospital of the Ruhr-University of Bochum, 4Department of Infectious Diseases, University of Duisburg-Essen, University Hospital Essen Hufelandstr
In this manuscript, a protocol is presented to perform FACS-based measurement of BK-polyomavirus transcriptional activity by using HEK293T cells transfected with a bidirectional reporter plasmid expressing tdTomato and eGFP. This method further allows to quantitatively determine the influence of novel compounds on viral transcription.
Medicine
Blood Circuit Reconstruction in an Abdominal Mouse Heart Transplantation Model
Decheng Yin *1,2, Jian Fu *1,3, Ida Allabauer 1, Oliver Witzke 4, Song Rong 5, André Hoerning 1
1Department of Pediatric and Adolescent Medicine, University Hospital Erlangen, Friedrich-Alexander University Erlangen-Nürnberg, 2Department of Pediatric Surgery, Chengdu Women's and Children's Central Hospital, 3The Key Laboratory of Hainan Trauma and Disaster Rescue, The first affiliated Hospital of Hainan Medical University, 4Department of Infectious Diseases, West German Centre of Infectious Diseases, Universitätsmedizin Essen, University Duisburg-Essen, 5Department of Nephrology, Hannover Medical School
A novel technique for blood circuit reconstruction in a heterotopic abdominal mouse heart transplantation model is demonstrated.
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