Production of Membrane-Filtered Phase-Shift Decafluorobutane Nanodroplets from Preformed MicrobubblesDarrah A. Merillat 1, Arvin Honari 1, Shashank R. Sirsi 1,2
1Department of Bioengineering, The University of Texas at Dallas, 2Department of Radiology, The University of Texas Southwestern
This protocol describes a method of generating large volumes of lipid encapsulated decafluorobutane microbubbles using probe-tip sonication and subsequently condensing them into phase-shift nanodroplets using high-pressure extrusion and mechanical filtration.
