Oncode Institute (NL)
2 ARTICLES PUBLISHED IN JoVE
Biology
Dendra2 Photoswitching through the Mammary Imaging Window
Bojana Gligorijevic 1,2, Dmitriy Kedrin 1, Jeffrey E Segall 1, John Condeelis 1,2, Jacco van Rheenen 1,2,3
1Department of Anatomy and Structural Biology, Albert Einstein College of Medicine - Yeshiva University, 2Gruss Lipper Biophotonics Center, Albert Einstein College of Medicine - Yeshiva University, 3Hubrecht Institute-KNAW and University Medical Center Utrecht
Intravital photoswitching and tracking of Dendra2-labeled tumor cells through the Mammary Imaging Window is a technique which allows us to image the metastatic behavior of tumor cells in chosen tumor microenvironments over a timescale of days.
Biology
Longitudinal Intravital Microscopy Using a Mammary Imaging Window with Replaceable Lid
Larissa Mourao 1,2, Marta Ciwinska 1,2, Jacco van Rheenen 3, Colinda L. G. J. Scheele 1,2
1Center for Cancer Biology, VIB (BE), 2Department of Oncology, KU Leuven (BE), 3Netherlands Cancer Institute, Department of Molecular Pathology, Oncode Institute (NL)
This protocol describes a novel mammary imaging window with a replaceable lid (R.MIW). Intravital microscopy after implantation of the R.MIW allows for longitudinal and multi-day imaging of the healthy and diseased mammary gland with a cellular resolution during the different developmental stages.
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