Following the Dynamics of Structural Variants in Experimentally Evolved PopulationsMichael Finnegan 1, Jeanne Hamet 1, Erick Desmarais 2, Stéphanie Bedhomme 1
1CEFE, University of Montpellier, CNRS, EPHE, IRD, Montpellier, France, 2ISEM, University of Montpellier, CNRS, EPHE, IRD, Montpellier, France
We developed a cost-effective method to follow non-single nucleotide polymorphism allele dynamics that can easily be adapted to experimental evolution frozen archives. A triplet PCR technique was coupled with automated parallel capillary electrophoresis to quantify the relative frequency of an insertion allele over the course of experimental evolution.
