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3 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

Orthotopic Hind-Limb Transplantation in Rats

Robert Sucher^*1, Rupert Oberhuber^*1, Christian Margreiter¹, Guido Rumberg¹, Rishi Jindal², WP Andrew Lee², Raimund Margreiter¹, Johann Pratschke¹, Stefan Schneeberger¹, Gerald Brandacher¹

¹Department of Visceral, Transplant, and Thoracic Surgery, Daniel Swarovski Research Laboratory, Innsbruck Medical University, ²Department of Surgery, Division of Plastic and Reconstructive Surgery, University of Pittsburgh Medical Center

Here we describe the orthotopic rat hind-limb transplantation procedure, which seems to be the gold standard in vivo model for composite tissue allotransplantation research.

Medicine

Murine Cervical Heart Transplantation Model Using a Modified Cuff Technique

Rupert Oberhuber^*1, Benno Cardini^*1, Markus Kofler¹, Paul Ritschl¹, Robert Oellinger¹, Felix Aigner¹, Robert Sucher¹, Stefan Schneeberger¹, Johann Pratschke¹, Gerald Brandacher², Manuel Maglione¹

¹Center of Operative Medicine, Department of Visceral, Transplant and Thoracic Surgery, Innsbruck Medical University, ²Department of Plastic and Reconstructive Surgery, Johns Hopkins University School of Medicine

The murine cervical heart transplantation model is well suited for immunological as well as ischemia reperfusion injury studies. We modified the procedure using a non-suture cuff technique and performed more than 1,000 successful transplants with this approach.

Herein, we provide additional details of this technique to supplement the video.

Medicine

Orthotopic Hind Limb Transplantation in the Mouse

Georg J. Furtmüller^*1, Byoungchol Oh^*1, Johanna Grahammer^*2, Cheng-Hung Lin³, Robert Sucher⁴, Madeline L. Fryer¹, Giorgio Raimondi¹, W.P. Andrew Lee¹, Gerald Brandacher¹

¹Department of Plastic and Reconstructive Surgery, Vascularized Composite Allotransplantation (VCA) Laboratory, Johns Hopkins University School of Medicine, ²Department of Visceral, Transplant and Thoracic Surgery, Innsbruck Medical University, ³Center for Vascularized Composite Allotransplantation, Department of Plastic and Reconstructive Surgery, Chang Gung Memorial Hospital and School of Medicine, ⁴Department of General, Visceral and Transplant Surgery, Charite Berlin

This novel model for orthotopic hind limb transplantation in the mouse, applying a non-suture cuff technique for super-microvascular anastomosis, provides a powerful tool for in vivo mechanistic immunological research related to vascularized composite allotransplantation (VCA).