biology

Biology

cancer research

Cancer Research

biological techniques

Biological Techniques

immunology

Immunology

neuroscience

Neuroscience

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jove encyclopedia of experiments

JoVE Encyclopedia of Experiments

Online video encyclopedias of advanced research experiments for scientists in academia and biotech

Research

genome editing techniques

This collection features techniques used for genome editing in cultured bacterial, animal, and plant cells and in vivo animal models. These methods highlight the applications of CRISPR technology, Zinc finger nucleases, TALENs, transposition and recombination events, shRNA, siRNA, and vector-based transformations to achieve gene knock-in, gene knock-out, and gene regulations.

Genome Editing Techniques

gene transfer techniques

This collection features gene transfer methods, including viral vector-mediated transduction and non-viral vector-mediated physical and chemical transfection techniques to deliver genetic material in cultured cells and in vivo animal models at their embryonic and adult stages. Highlights include transfection utilizing hydrodynamics, microinjection, biolistics, nucleofection, and magnetofection techniques, and transduction via retroviral, adenoviral, nanoblade, and lentiviral systems.

Gene Transfer Techniques

staining techniques

Staining Techniques

This collection features methods for staining biomolecules such as lipids, carbohydrates, and proteins; and different cell types ranging from individual plant or animal cells to various tissue sections. These methods help visualize and characterize a wide variety of biological samples by improving their contrast.

chromatography techniques

This collection explains the mechanisms behind separating and purifying various analytes such as proteins, lipids, carbohydrates, antibody-drug conjugates, and small molecules from complex mixtures using column, capillary, or plate-based systems. The highlights include chromatographic separation methods using ion exchange, standard and reverse-phase, affinity, size-exclusion, gas-based, and thin-layer dependent techniques.

Chromatography Techniques

electrophoresis techniques

This collection highlights the mechanisms behind various electrophoresis techniques to separate biomolecules such as DNA, RNA, peptides, individual proteins, protein complexes, and amyloids. These methods utilize variants of agarose gel- or polyacrylamide gel-based matrices to separate biomolecules under the influence of an electric field.

Electrophoresis Techniques

assay techniques

This collection features various bioanalytical techniques to assess the activity, potency, or localization of test substances in living organisms performed at the macromolecular, sub-cellular, cellular, or organism level. Assay outcomes are detected directly or indirectly by visualizing the responses via colorimetric, enzymatic, or electrophysiological measurement methods.

Assay Techniques

pcr techniques

The focus of this collection extends to a range of applications and methods that utilize Polymerase Chain Reaction (PCR) to identify and examine genetic markers, mutations, and sequences. These techniques encompass mutation detection, annealing temperature optimization, genetic marker quantification, infection diagnosis, enumeration of infective particles, nucleic acid amplification, analysis of epigenetic modifications, and detection of transcript variants.

PCR Techniques

blot techniques

Blot techniques encompass a variety of meticulous methods employed for the quantification and detection of biomolecules, spanning DNA, RNA, and proteins, contributing to a comprehensive understanding of biological systems. This collection features diverse applications, including the precise quantification and detection of biomolecules, assessing genetic material, examining epigenetic modifications, and measuring protein expression levels.

Blot Techniques

biomolecular interaction detection techniques

This collection features various methods and techniques used to study protein-protein interactions. These approaches encompass scientific tools and technologies, such as biosensors, imaging techniques, assays, and spectroscopy, that provide insights into the intricate world of protein interactions and their roles in biological systems, enabling a deeper understanding of the events that transpire at the molecular level. These interactions are fundamental in numerous cellular activities, including signal transduction, enzymatic reactions, gene regulation, and structural organization.

Biomolecular Interaction Detection Techniques

spectroscopy techniques

This collection highlights various spectroscopy techniques that are relevant in molecular biology. Based on the principle that molecules absorb or emit light at specific wavelengths or frequencies, spectroscopy allows for the measurement of light absorption, emission, or scattering. These techniques provide valuable insights into the composition, structure, interactions, and dynamics of molecules involved in biological processes.

Spectroscopy Techniques

microscopy techniques

The collection comprises an array of advanced experimental techniques in the field of microscopy and imaging, aimed at investigating various biological and cellular processes. These techniques enable researchers to visualize and study dynamic cellular events, molecular interactions, and structural features with high resolution and specificity..

Microscopy Techniques

tirf microscopy to visualize actin and microtubule coupling dynamics

tirf-microscopy-to-visualize-actin-and-microtubule-coupling-dynamics

TIRF Microscopy to Visualize Actin and Microtubule Coupling Dynamics

Source: Henty-Ridilla, J.L., Visualizing Actin and Microtubule Coupling Dynamics In Vitro by Total Internal Reflection Fluorescence (TIRF) Microscopy. J. ...

lattice light sheet microscopy to visualize receptor-ligand interactions in live cells

lattice-light-sheet-microscopy-to-visualize-receptor-ligand

Lattice Light Sheet Microscopy to Visualize Receptor-Ligand Interactions in Live Cells

Source: Rosenberg, J., et al. Visualizing Surface T-Cell Receptor Dynamics Four-Dimensionally Using Lattice Light-Sheet Microscopy. J. Vis. Exp. (2020).
...

darkfield microscopy to visualize diffusional dynamics of nanorods on cell membrane

darkfield-microscopy-to-visualize-diffusional-dynamics-nanorods-on

Darkfield Microscopy to Visualize Diffusional Dynamics of Nanorods on Cell Membrane

Source: Ge, F., et al. Visualizing Diffusional Dynamics of Gold Nanorods on Cell Membrane using Single Nanoparticle Darkfield Microscopy. J. Vis. Exp. ...

interference reflection microscopy for label-free visualization of microtubule dynamics

interference-reflection-microscopy-for-label-free-visualization

Interference Reflection Microscopy for Label-Free Visualization of Microtubule Dynamics

Source: Mahamdeh, M., et al. Implementation of Interference Reflection Microscopy for Label-free, High-speed Imaging of Microtubules. J. Vis. Exp. 
...

static atomic force microscopy to visualize and characterize assembled nucleosomes

static-atomic-force-microscopy-to-visualize-characterize-assembled

Static Atomic Force Microscopy to Visualize and Characterize Assembled Nucleosomes

Source: Stormberg, T., et al. Probing The Structure And Dynamics Of Nucleosomes Using Atomic Force Microscopy Imaging. J. Vis. Exp.(2019)
In this video, ...

high-speed time-lapse atomic force microscopy to capture nucleosome dynamics

high-speed-time-lapse-atomic-force-microscopy-to-capture-nucleosome

High-Speed Time-Lapse Atomic Force Microscopy to Capture Nucleosome Dynamics

Source: Stumme-Diers, M. P., et al., Probing The Structure And Dynamics Of Nucleosomes Using Atomic Force Microscopy Imaging.&#160;J. Vis. ...

transmission electron microscopy to quantify glycogen distribution in human skeletal muscles

transmission-electron-microscopy-to-quantify-glycogen-distribution

Transmission Electron Microscopy to Quantify Glycogen Distribution in Human Skeletal Muscles

Source: Jensen, R., et al. Quantification of Subcellular Glycogen Distribution in Skeletal Muscle Fibers using Transmission Electron Microscopy. J. Vis. ...

quantification of membrane ruffle formation using scanning electron microscopy

quantification-membrane-ruffle-formation-using-scanning-electron

Quantification of Membrane Ruffle Formation Using Scanning Electron Microscopy

Source: Ahn, W. et al., Visualizing Membrane Ruffle Formation using Scanning Electron Microscopy.&#160;J. Vis. Exp. (2021)
This video demonstrates sample ...

intravital fluorescence microscopy to study microvascular thrombus formation

intravital-fluorescence-microscopy-to-study-microvascular-thrombus

Intravital Fluorescence Microscopy to Study Microvascular Thrombus Formation

Source: Str&#252;der, D., et al. Intravital Microscopy and Thrombus Induction in the Earlobe of a Hairless Mouse. J. Vis. Exp. (2017)
This video ...

immunofluorescence microscopy for the analysis of dna double-strand breaks

immunofluorescence-microscopy-for-analysis-dna-double-strand

Immunofluorescence Microscopy for the Analysis of DNA Double-Strand Breaks

Source: Popp, H. D., et al. Immunofluorescence Microscopy of &#947;H2AX and 53BP1 for Analyzing the Formation and Repair of DNA Double-strand Breaks. J. ...

Encyclopedia Of Experiments - biological techniques