biology

Biology

cancer research

Cancer Research

biological techniques

Biological Techniques

immunology

Immunology

neuroscience

Neuroscience

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jove encyclopedia of experiments

JoVE Encyclopedia of Experiments

Online video encyclopedias of advanced research experiments for scientists in academia and biotech

Research

breast cancer

This collection features biomedical research methods employed in research laboratories to advance breast cancer prevention, detection, and treatment.

Breast Cancer

lung cancer

This collection highlights some of the latest protocols in lung cancer research. The collection covers techniques relating to the generation of lung cancer animal models, cancer cell characterization, treatment and detection strategies, in vitro culture studies, assays and isolation techniques that facilitate lung cancer research.

Lung Cancer

leukemia

Leukemia reflects cancer primarily resulting from an elevated number of white blood cells in the body. This collection features a set of in vitro assay techniques to analyze leukemic cell growth and metabolism, cell isolation and culture methods, approaches to characterize and study cellular morphology, and genetic manipulation procedures.

Leukemia

pancreatic cancer

The collection features protocols demonstrating the generation of pancreatic animal models, genetic engineering of pancreatic cancer cells, methods for in vitro modeling of pancreatic cancer, cell isolation & characterization techniques, and tumor cell behavior and response studies.

Pancreatic Cancer

colorectal cancer

Colorectal cancer originates within the colon or rectal regions of organisms. This collection highlights some fundamental research techniques in colorectal cancer relating to screening tests, genetic analysis, studies on the gut microbiome, therapeutic interventions, animal model generation, assay techniques, and cell behavior & metastatic responses.

Colorectal Cancer

skin cancer

This collection focusses on research approaches relating to melanoma and other types of skin cancer. The protocols feature techniques relevant to developing in vitro and in vivo skin carcinogenesis and metastatic models, cell culturing, skin regeneration approaches, genetic manipulation and sensitivity studies using skin cells, and assay techniques.

Skin Cancer

gynecologic cancer

This collection features protocols relating to the prevention, detection, and treatment of cancer originating from cells or regions of the female reproductive system. The protocols are relevant for cancer-targeting and detection approaches, metabolic assessment of cancer cells, developing in vivo and metastatic animal models, in vitro cell culture and assay techniques, and transplantation experiments.

Gynecologic Cancer

prostate cancer

This collection features techniques employed in prostate cancer research relating to cancer cell/organoid culture, processing, and staining, cell and tissue sampling, organ extractions, in vivo & in vitro cancer modeling, and cancer proteomics studies.

Prostate Cancer

gastrointestinal cancer

Gastrointestinal cancers include various tumor types originating in the regions or organs associated with the gastrointestinal tract. The collection comprises research protocols on cell isolation, culturing, and adhesion studies, tissue staining and histological analysis, DNA methylation studies, tissue preservation methods, exosome analysis, and generation of animal models.

Gastrointestinal Cancer

head and neck cancer

Head and neck cancers constitute carcinomas originating in and around the mucosal surfaces of the head and neck regions. This collection features protocols relating to cancer modeling in vitro, in ovo, and in vivo, cell isolation and culture, fluorescent in situ hybridization-related techniques, generation of decellularized tissue matrices, and drug-resistance characterization assays.

Head and Neck Cancer

urinary tract cancer

This collection features protocols relating to cancers originating in the regions or organs of the urinary or renal system. The techniques include organs, tissue, and cell harvesting procedures, organotypic culturing approaches, in vivo cancer modeling studies, decellularized tissue matrix generation, stem cell-related and electrophysiology studies, genetic-engineering strategies, tissue staining and imaging techniques, and protein expression analysis.

Urinary Tract Cancer

cancers of the nervous system

This collection features protocols relating to cancers originating in the nervous system. The techniques include intra-brain injections, in-vivo and in-vitro tumor modeling, cell and organelle culturing, extracellular vesicle harvesting and purification methodologies, proteomic analysis, organ isolation and preservation methods, cell migration assays, tissue engineering methods, and novel gene delivery approaches.

Cancers of the Nervous System

procedures and techniques

Procedures and techniques

in vitro studies

In vitro studies

orthotopic injection into the mammary fat pad:  establishing breast cancer in mice

orthotopic-injection-into-mammary-fat-pad-establishing-breast-cancer

Orthotopic Injection into the Mammary Fat Pad:  Establishing Breast Cancer in Mice

Source: Zhang, G. L., et al. Orthotopic Injection of Breast Cancer Cells into the Mice Mammary Fat Pad.&#160;J. Vis. Exp. (2019).
In this article we ...

lymphedema ultrasonography: a technique to measure the change in thickness of an affected tissue

lymphedema-ultrasonography-technique-to-measure-change-thickness-an

Lymphedema Ultrasonography: A Technique to Measure the Change in Thickness of an Affected Tissue

Source: Jeon, Y., et al. Ultrasonographic Evaluation of Breast Cancer-related Lymphedema.&#160;J. Vis. Exp. (2017).
This video utilizes ultrasonography ...

orthotopic injection: implanting tissue specific cancer cells into an adult mouse

orthotopic-injection-implanting-tissue-specific-cancer-cells-into-an

Orthotopic Injection: Implanting Tissue Specific Cancer Cells into an Adult Mouse

Source: Katsuta, E., et al. Generating a Murine Orthotopic Metastatic Breast Cancer Model and Performing Murine Radical Mastectomy.&#160;J. Vis. ...

radical mastectomy: surgical removal of the entire mammary gland from a mouse to study cancer progression

radical-mastectomy-surgical-removal-entire-mammary-gland-from-mouse

Radical Mastectomy: Surgical Removal of the Entire Mammary Gland from a Mouse to Study Cancer Progression

Source: Katsuta, E., et al. Generating a Murine Orthotopic Metastatic Breast Cancer Model and Performing Murine Radical Mastectomy. J. Vis. Exp (2018).
...

intraductal injection: delivering injection mix into the ducts of the mouse mammary gland

intraductal-injection-delivering-injection-mix-into-ducts-mouse

Intraductal Injection: Delivering Injection Mix into the Ducts of the Mouse Mammary Gland

Source: Xiang, D., et al. Modeling Breast Cancer via an Intraductal Injection of Cre-expressing Adenovirus into the Mouse Mammary Gland. J. Vis. Exp ...

zpdx-analysis of invasiveness: investigating invasive behavior of metastatic cancer cells in zebrafish embryo xenografts

zpdx-analysis-invasiveness-investigating-invasive-behavior-metastatic

zPDX-Analysis of Invasiveness: Investigating Invasive Behavior of Metastatic Cancer Cells in Zebrafish Embryo Xenografts

Source: Ren, J. et al. Invasive Behavior of Human Breast Cancer Cells in Embryonic Zebrafish. J. Vis. Exp. (2017).
This video describes a microinjection ...

portal vein injection: a method to study cancer metastasis to the liver

portal-vein-injection-a-method-to-study-cancer-metastasis-to-the-liver

Portal Vein Injection: A Method to Study Cancer Metastasis to the Liver

Source: Goddard, E. T., et al. A Portal Vein Injection Model to Study Liver Metastasis of Breast Cancer. J. Vis. Exp. (2016).
This video describes a ...

india ink inflation: a staining method to visualize tumor nodules

india-ink-inflation-a-staining-method-to-visualize-tumor-nodules

India Ink Inflation: A Staining Method to Visualize Tumor Nodules

Source: Paschall, A. V. et al. An Orthotopic Mouse Model of Spontaneous Breast Cancer Metastasis. J. Vis. Exp. (2016).
This video describes india ink ...

spatial measurement of tumor interstitial fluid pressure: a method to measure the interstitial fluid pressure

spatial-measurement-tumor-interstitial-fluid-pressure-method-to

Spatial Measurement of Tumor Interstitial Fluid Pressure: A Method to Measure the Interstitial Fluid Pressure

Source: Stapleton et. al., Measurements of Perfusion, Interstitial Fluid Pressure and Liposomes Accumulation in Solid Tumors. J. Vis. Exp. (2016).
This ...

rabbit intraductal injection: localized delivery of solution of interest into the rabbit mammary gland

rabbit-intraductal-injection-localized-delivery-solution-interest

Rabbit Intraductal Injection: Localized Delivery of Solution of Interest into the Rabbit Mammary Gland

Source: Clark et. al., Intraductal Delivery to the Rabbit Mammary Gland. J. Vis. Exp. (2017).
In this video we describe a technique of administering a ...

mechanical dissociation: a method to obtain viable cells from a tissue

mechanical-dissociation-a-method-to-obtain-viable-cells-from-a-tissue

Mechanical Dissociation: A Method to Obtain Viable Cells from a Tissue

Source: Garaud et. al., A Simple and Rapid Protocol to Non-enzymatically Dissociate Fresh Human Tissues for the Analysis of Infiltrating Lymphocytes. J. ...

sample preparation for metabolomics: a method to prepare cell samples for metabolite profiling

sample-preparation-for-metabolomics-method-to-prepare-cell-samples

Sample Preparation for Metabolomics: A Method to Prepare Cell Samples for Metabolite Profiling

Source: Zhao, Y. C. et. al., Systems Biology of Metabolic Regulation by Estrogen Receptor Signaling in Breast Cancer. J. Vis. Exp. (2016).
This video ...

tail vein injection: a method to administer cancer cells for metastatic studies in a mouse model

tail-vein-injection-method-to-administer-cancer-cells-for-metastatic

Tail Vein Injection: A Method to Administer Cancer Cells for Metastatic Studies in a Mouse Model

Source: Warren et. al., Combined Use of Tail Vein Metastasis Assays and Real-Time In Vivo Imaging to Quantify Breast Cancer Metastatic Colonization and ...

decellularizing mammary fat pads: deriving extracellular matrix hydrogels from mammary fat pads

decellularizing-mammary-fat-pads-deriving-extracellular-matrix

Decellularizing Mammary Fat Pads: Deriving Extracellular Matrix Hydrogels from Mammary Fat Pads

Source: Alves et. al., Studying Normal Tissue Radiation Effects using Extracellular Matrix Hydrogels. J. Vis. Exp. (2019).
This video describes a ...

generation of primary mammospheres: a technique to determine cell potency

generation-primary-mammospheres-technique-to-determine-cell

Generation of Primary Mammospheres: A Technique to Determine Cell Potency

Source: Lombardo et. al., Mammosphere Formation Assay from Human Breast Cancer Tissues and Cell Lines. J. Vis. Exp. (2015).
This video describes isolating ...

transduction to label pdx tumor cells: introducing lentivirus expressing fluorescent marker into the tumor cell in vitro

transduction-to-label-pdx-tumor-cells-introducing-lentivirus

Transduction to Label PDX Tumor Cells: Introducing Lentivirus Expressing Fluorescent Marker into the Tumor Cell In Vitro

Source: Hanna, C., et al. Labeling of Breast Cancer Patient-derived Xenografts with Traceable Reporters for Tumor Growth and Metastasis Studies. J. Vis. ...

co-culturing bc cells: culturing breast cancer cells with bone fragments to study cancer cell proliferation

co-culturing-bc-cells-culturing-breast-cancer-cells-with-bone

Co-culturing BC Cells: Culturing Breast Cancer Cells with Bone Fragments to Study Cancer Cell Proliferation

Source: Templeton, Z. S., et al. Methods for Culturing Human Femur Tissue Explants to Study Breast Cancer Cell Colonization of the Metastatic Niche. J. ...

3d co-culture with direct interaction: co-culturing cancer cells with monocytes to study their interaction

3d-co-culture-with-direct-interaction-co-culturing-cancer-cells-with

3D Co-culture with Direct Interaction: Co-culturing Cancer Cells with Monocytes to Study Their Interaction

Source: Espinoza-S&#225;nchez, et al. Analyzing the Communication Between Monocytes and Primary Breast Cancer Cells in an Extracellular Matrix Extract ...

organ conditioned medium: generating medium from harvested organ cells for metastatic cancer studies

organ-conditioned-medium-generating-medium-from-harvested-organ-cells

Organ Conditioned Medium: Generating Medium from Harvested Organ Cells for Metastatic Cancer Studies

Source: Piaseczny, M. M., et al. Generation of Organ-conditioned Media and Applications for Studying Organ-specific Influences on Breast Cancer Metastatic ...

3d culture basement membrane assay: culturing cancer cells on 3d basement membrane protein matrix to study cell invasion

3d-culture-basement-membrane-assay-culturing-cancer-cells-on-3d

3D Culture Basement Membrane Assay: Culturing Cancer Cells on 3D Basement Membrane Protein Matrix to Study Cell Invasion

Source: Cvetkovi&#263;, D., et al. Quantification of Breast Cancer Cell Invasiveness Using a Three-dimensional (3D) Model. J. Vis. Exp. (2014).
This video ...

soft agar colony formation assay: a method to test effects of novel compounds on cancer cell proliferation

soft-agar-colony-formation-assay-method-to-test-effects-novel

Soft Agar Colony Formation Assay: A Method to Test Effects of Novel Compounds on Cancer Cell Proliferation

Source: Horibata A. et. al.,  Utilization of the Soft Agar Colony Formation Assay to Identify Inhibitors of Tumorigenicity in Breast Cancer Cells. J. Vis. ...

Encyclopedia Of Experiments - cancer research