Equipment Giriş <ol><li> Doku uygulanabilir tek hücre süspansiyonları içine menekşe C Tüpler kullanarak, hafifçe ayrışmış. </li><li> PBS / BSA EDTA tampon hücreleri ayırmak kullanılır. Tampon BSA Stok çözelti, EDTA içeren Çözüm Durulama autoMACS 20 kısım 1 kısım ekleyerek hazırlanır. </li><li> MACS Ön Ayırma Filtreler başarılı MACS ayrılması ve daha sonraki analiz teşvik ayrışmış doku örnekleri tüm hücre kümeleri, böylece kullanılır. </li></ol> Dalak hücreleri Dissociating <ol><li> Tüm dalak dalak gentleMACS Dissociator kullanarak tek hücre süspansiyonları disscociated. </li><li> Prosedürü başlatmak için, dalak, kitle göre tampon hacmi ile C Tüp yük. Genç bir yetişkin erkek BALB / c fare sağlıklı bir dalak 80-120 mg ağırlığındadır. Bu kitle her 1 ila 2 dalak 3 mL tampon gerektirir. Enfeksiyonları ile büyük hayvan veya hayvanların dalak genellikle büyük ve tampon tartılır olmalıdır. Tampon hacimleri buna göre ölçeklenmiş olmalıdır. 10 ml toplam tampon hacmi aşmayın. </li><li> 3 ml tampon içeren C Tüp disseke dalak ekleyin. </li><li> C Tüp kapağı sıkı olduğundan emin olun ve kapağı aşağı dissociator C Tüp takmak. </li><li> Dissociator kadar güç. 1-2 dalak için program m_spleen_01 seçin. Programını tamamlamak için 56 saniye sürer. Dalak, sayı büyükse, program m_spleen_04 kullanın, bu program 720 mg dalak doku için kullanılabilir. </li><li> Programın tamamlanmasından sonra yerli dalak doku splenocyte süspansiyon ayrışmış. </li><li> Yaklaşık 30 saniye boyunca oda sıcaklığında 300xg tüm hücre süspansiyonu aşağı spin. Bu rotor ve stator C Boru altında toplanan tüm hücreleri serbest olmasını sağlayacaktır. </li><li> 1000 mikrolitrelik pipetter kullanarak septum-C Tüp kapağı kapalı açılış ile örnek çıkarın. </li></ol> Filtrasyon adım <ol><li> Filtrasyon adım başlamak için, Pre-Ayırma Filtre ya da 30 mikron gözenek boyutu ile bir hücre süzgeç ayrışmış doku geçerlidir. Süzgeç fazla 2 dalak, 1-2 dalak ya da 50 ml tüp 15 ml tüp uygun olmalıdır. </li><li> Hücre süspansiyonu geçmesine izin verin. Daha sonra 5 ml tampon uygulayarak filtre yıkayın. </li><li> Şimdi, bir dalak hücre pelletini tüm splenositlerin toplamak için 10 dakika boyunca oda sıcaklığında, 300xg hücre süspansiyonu ile tüp santrifüj. </li><li> Şimdi, ayrışmış splenositlerin bir pelet, süpernatantı aspirat ve tamponu uygun bir miktarda hücre pelletini tekrar süspansiyon gerekir. </li></ol> Dalak ayrılma gentleMACS Dissociator kullanarak canlı splenositlerin MACSQuant Analyzer kullanarak flow sitometri gösterdiği yüksek bir yüzdesi verecektir. Ölü hücreler propidium iyodür tarafından lekeli. Verilen örnekte, ileri yan dağılım nokta arsa hazırlanan splenositlerin gösterir.

Tüm protokolleri ve veri sayfaları mevcuttur<a href="http://www.miltenyibiotec.com"&gt; Http://www.miltenyibiotec.com.</a&gt;.

GentleMACS Dissociator sıkıcı ve sıklıkla ağrılı manuel dissociations önlemek için, kontrollü ve tekrarlanabilir koşullar altında çok etkin bir şekilde doku ayırmak sağlar. Doku disosiasyon prosedürü yüksek canlılık oranı ile hücre ve verim hücre süspansiyonları yumuşaktır. C Tüpleri ile birlikte gentleMACS Dissociator, çeşitli dokuların tek hücre süspansiyonları hazırlanması için tasarlanmıştır. Cihaz fare dalak, karaciğer, akciğer veya sinir doku ayırmak önceden belirlenmiş programları bir dizi ile donatılmıştır. Çeşitli programların ve protokollerin ek dokuların sürekli kapsayacak şekilde genişletildi olacaktır.

<table><tbody><tr><td>gentleMACS&amp;#8482; Starting Kit</td><td>Miltenyi Biotec</td><td>130-093-235</td><td>&lt;a href=&quot;http://www.miltenyibiotec.com/en/PG_709_763_gentleMACS_Dissociator.aspx&quot;&gt;http://www.miltenyibiotec.com/en/PG_709_763_gentleMACS_Dissociator.aspx&lt;/a&gt;</td></tr><tr><td>C Tubes</td><td>Miltenyi Biotec</td><td>130-093-237</td><td>&lt;a href=&quot;http://www.miltenyibiotec.com/en/PG_709_765_gentleMACS_C_Tubes.aspx&quot;&gt;http://www.miltenyibiotec.com/en/PG_709_765_gentleMACS_C_Tubes.aspx&lt;/a&gt;</td></tr><tr><td>13 mL Tubes</td><td>Miltenyi Biotec</td><td>130-091-596</td><td>&lt;a href=&quot;http://www.miltenyibiotec.com/en/PG_34_751_13_mL_Tubes_for_LS_Columns.aspx&quot;&gt;http://www.miltenyibiotec.com/en/PG_34_751_13_mL_Tubes_for_LS_Columns.aspx&lt;/a&gt;</td></tr><tr><td>autoMACS&amp;#8482; Rinsing Solution </td><td>Miltenyi Biotec</td><td>130-091-222</td><td>&lt;a href=&quot;http://www.miltenyibiotec.com/en/PG_134_155_autoMACS_Rinsing_Solution.aspx&quot;&gt;http://www.miltenyibiotec.com/en/PG_134_155_autoMACS_Rinsing_Solution.aspx&lt;/a&gt;</td></tr><tr><td>MACS&amp;#174; BSA Stock Solution</td><td>Miltenyi Biotec</td><td>130-091-376</td><td>&lt;a href=&quot;http://www.miltenyibiotec.com/en/PG_33_295_MACS_BSA_Stock_Solution.aspx&quot;&gt;http://www.miltenyibiotec.com/en/PG_33_295_MACS_BSA_Stock_Solution.aspx&lt;/a&gt;</td></tr><tr><td>Pre-Separation Filters </td><td>Miltenyi Biotec</td><td>130-041-407</td><td>&lt;a href=&quot;http://www.miltenyibiotec.com/en/PG_34_247_Pre_Separation_Filters.aspx&quot;&gt;http://www.miltenyibiotec.com/en/PG_34_247_Pre_Separation_Filters.aspx&lt;/a&gt;</td></tr><tr><td>MACSQuant Analyzer</td><td>Miltenyi Biotec</td><td>130-092-197</td><td></td></tr><tr><td>Propidium Iodide Solution</td><td>Miltenyi Biotec</td><td>130-093-233</td><td>&lt;a href=&quot;http://www.miltenyibiotec.com/en/PG_337_744_Propidium_Iodide_Solution.aspx&quot;&gt;http://www.miltenyibiotec.com/en/PG_337_744_Propidium_Iodide_Solution.aspx&lt;/a&gt;</td></tr></tbody></table>

preparation of single-cell suspensions from mouse spleen with the gentlemacs dissociator

Tek hücre süspansiyonları hücre ayırma, hücre analizi ve hücre kültürü deneyleri için bir önkoşuldur. GentleMACS Dissociator sıkıcı ve sıklıkla ağrılı manuel dissociations önlemek için kontrollü ve tekrarlanabilir koşullar altında çok etkin bir şekilde doku ayırmak sağlar. GentleMACS Dissociator optimal, kullanıcı güvenliği ile zaman kazandıran ve standardizasyon birleştirerek, fare dalak ayrılabilir. Bu video gentleMACS Dissociator, mekanik, canlı hücre süspansiyonları üretmek için özel tüpler kullanılarak dokuların bozabilir otomatik bir tezgah üstü cihazı kullanarak fare dalak ayırmak anlatılmaktadır. Disosiasyon ardından, dalak, süzülür ve başka uygulamalar için yeniden süspanse.

Watch this Scientific Journal Video about Preparation of Single-Cell Suspensions from Mouse Spleen with the gentleMACS Dissociator at JoVE.com

Preparation of Single-Cell Suspensions from Mouse Spleen with the gentleMACS Dissociator

Bu video gentleMACS Dissociator kullanarak fare dalak tek hücre süspansiyonları hazırlamak için otomatik doku ayrılma için basit, zaman tasarrufu sağlayan bir teknik açıklar.

Fare Dalak gentleMACS Dissociator Tek Hücreli Cezalar hazırlanması

Single-cell suspensions are a prerequisite for experiments in cell separation, cell analysis and cell culture.  To avoid tedious and often painful manual ...

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preparation-single-cell-suspensions-from-mouse-spleen-with-gentlemacs

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Biology

37.7K Görüntüleme.  Miltenyi Biotec,GmbH.  中文 JapaneseVersion 

Video: Preparation of Single-Cell Suspensions from Mouse Spleen with the gentleMACS Dissociator

Preparation of Myeloid Derived Suppressor Cells (MDSC) from Naive and Pancreatic Tumor-bearing Mice using Flow Cytometry and Automated Magnetic Activated Cell Sorting (AutoMACS)

MDSC are a heterogeneous population of immature macrophages, dendritic cells and granulocytes that accumulate in lymphoid organs in pathological conditions including parasitic infection, inflammation, traumatic stress, graft-versus-host disease, diabetes and cancer1-7. In mice, MDSC express Mac-1 (CD11b) and Gr-1 (Ly6G and Ly6C) surface antigens7. It is important to note that MDSC are well studied in various tumor-bearing hosts where they are significantly expanded and suppress anti-tumor immune responses compared to na&iuml;ve counterparts7-10. However, depending on the pathological condition, there are different subpopulations of MDSC with distinct mechanisms and targets of suppression11,12. Therefore, effective methods to isolate viable MDSC populations are important in elucidating their different molecular mechanisms of suppression in vitro and in vivo.
Recently, the Ghansah group has reported the expansion of MDSC in a murine pancreatic cancer model. Our tumor-bearing MDSC display a loss of homeostasis and increased suppressive function compared to na&iuml;ve MDSC 13. MDSC percentages are significantly less in lymphoid compartments of na&iuml;ve vs. tumor-bearing mice. This is a major caveat, which often hinders accurate comparative analyses of these MDSC. Therefore, enriching Gr-1+ leukocytes from na&iuml;ve mice prior to Fluorescence Activated Cell Sorting (FACS) enhances purity, viability and significantly reduces sort time. However, enrichment of Gr-1+ leukocytes from tumor-bearing mice is optional as these are in abundance for quick FACS sorting. Therefore, in this protocol, we describe a highly efficient method of immunophenotyping MDSC and enriching Gr-1+ leukocytes from spleens of na&iuml;ve mice for sorting MDSC in a timely manner. Immunocompetent C57BL/6 mice are inoculated with murine Panc02 cells subcutaneously whereas na&iuml;ve mice receive 1XPBS. Approximately 30 days post inoculation; spleens are harvested and processed into single-cell suspensions using a cell dissociation sieve. Splenocytes are then Red Blood Cell (RBC) lysed and an aliquot of these leukocytes are stained using fluorochrome-conjugated antibodies against Mac-1 and Gr-1 to immunophenotype MDSC percentages using Flow Cytometry. In a parallel experiment, whole leukocytes from na&iuml;ve mice are stained with fluorescent-conjugated Gr-1 antibodies, incubated with PE-MicroBeads and positively selected using an automated Magnetic Activated Cell Sorting (autoMACS) Pro Separator. Next, an aliquot of Gr-1+ leukocytes are stained with Mac-1 antibodies to identify the increase in MDSC percentages using Flow Cytometry. Now, these Gr1+ enriched leukocytes are ready for FACS sorting of MDSC to be used in comparative analyses (na&iuml;ve vs. tumor- bearing) in in vivo and in vitro assays.

Preparation of Myeloid Derived Suppressor Cells MDSC from Naive and Pancreatic Tumor-bearing Mice using Flow Cytometry and Automated Magnetic Activated Cell Sorting AutoMACS

This is a rapid and comprehensive method of immunophenotyping Myeloid Derived Suppressor Cells (MDSC) and enriching Gr-1+ leukocytes from mouse spleens. This method uses flow cytometry and AutoMACS Cell Sorting to enrich for viable Gr-1+ leukocytes prior to FACS sorting of MDSC for use in vivo and in vitro assays.

Akım Sitometri ve Otomatik Manyetik Aktif Hücre Sıralama (AutoMACS) kullanarak Naive ve Pankreas Tümör taşıyan fareler ile myeloid türevi bastırıcı hücreler (MDSC) hazırlanması

MDSC are a heterogeneous population of immature macrophages, dendritic cells and granulocytes that accumulate in lymphoid organs in pathological ...

Tıp

Standardized Preparation of Single-Cell Suspensions from Mouse Lung Tissue using the gentleMACS Dissociator

The preparation of single-cell suspensions from tissues is an important prerequisite for many experiments in cellular research. The process of dissociating whole organs requires specific parameters in order to obtain a high number of viable cells in a reproducible manner. The gentleMACS Dissociator optimizes this task with a simple, practical protocol. The instrument contains pre-programmed settings that are optimized for the efficient but gentle dissociation of a variety of tissue types, including mouse lungs. In this publication the use of the gentleMACS Dissociator on lung tissue derived from mice is demonstrated.

Dissociating cells from specific tissue types requires specific parameters for tissue agitation to obtain a high volume of viable, culturable cells. The Miltenyi gentleMACS dissociator optimizes this task with a simple, practical protocol. In this publication the use of this apparatus on lung tissue is explained.

Standardize Hazırlık gentleMACS Dissociator kullanarak Fare Akciğer Doku Tek Hücre Cezalar

The preparation of single-cell suspensions from tissues is an important prerequisite for many experiments in cellular research. The process of ...

Biyoloji

Generation of Single-Cell Suspensions from Mouse Neural Tissue

Within the nervous system, hundreds of neuronal and glial cell types have been described. Each specific cell type in the brain or spinal cord has a repertoire of cell surface molecules, or molecular determinants, through which it can be identified and characterized. Currently, robust cell identification and separation technologies require single-cell preparations to be generated while simultaneously limiting cell death and destruction of characteristic surface protein. The gentleMACS Dissociator, when used in combination with trypsin or papain-based dissociation kits, can effectively and gently dissociate brain tissue while preserving antigen epitopes and limiting cell loss. Standardized preparation of single-cell suspensions is achieved using C Tubes and optimized, preset gentleMACS Programs. Once generated, single-cell suspensions can be treated with monoclonal conjugates like Anti-Prominin-1 MicroBeads, which identify neural progenitors, or purified further using Myelin Removal Beads.

Dissociating cells from specific tissue types requires specific parameters for tissue aggitation to obtain a high volume of viable, culturable cells. The Miltenyi gentleMACS Dissociator optimizes this task with a simple, practical protocol. In this publication the use of this apparatus on nerual tissue is explained.

Fare Sinir Doku Hücre Tek Cezalar Üretimi

Within the nervous system, hundreds of neuronal and glial cell types have been described. Each specific cell type in the brain or spinal cord has a ...

Mouse Na&#239;ve CD4+ T Cell Isolation and In vitro Differentiation into T Cell Subsets

Antigen inexperienced (na&#239;ve) CD4+ T cells undergo expansion and differentiation to effector subsets at the time of T cell receptor (TCR) recognition of cognate antigen presented on MHC class II. The cytokine signals present in the environment at the time of TCR activation are a major factor in determining the effector fate of a na&#239;ve CD4+ T cell. Although the cytokine environment during na&#239;ve T cell activation may be complex and involve both redundant and opposing signals in vivo, the addition of various cytokine combinations during naive CD4+ T cell activation in vitro can readily promote the establishment of effector T helper lineages with hallmark cytokine and transcription factor expression. Such differentiation experiments are commonly used as a first step for the evaluation of targets believed to promote or inhibit the development of certain CD4+ T helper subsets. The addition of mediators, such as signaling agonists, antagonists, or other cytokines, during the differentiation process can also be used to study the influence of a particular target on T cell differentiation. Here, we describe a basic protocol for the isolation of na&#239;ve T cells from mouse and the subsequent steps necessary for polarizing na&#239;ve cells to various T helper effector lineages in vitro.

Mouse Na&#239;ve CD4+ T Cell Isolation and In vitro Differentiation into T Cell Subsets

Na&#239;ve CD4+ T cells polarize to various subsets depending on the environment at the time of activation. The differentiation of na&#239;ve CD4+ T cells to various effector subsets can be achieved in vitro through the addition of T cell receptor stimuli and specific cytokine signals.

Fare Naif CD4<sup&gt; +</sup&gt; T Hücre İzolasyonu ve<em&gt; In vitro</em&gt; T hücre alt içine Farklılaşma

Antigen inexperienced (na&#239;ve) CD4+ T cells undergo expansion and differentiation to effector subsets at the time of T cell receptor (TCR) recognition ...

İmmünoloji ve Enfeksiyon

An Efficient and High Yield Method for Isolation of Mouse Dendritic Cell Subsets

Dendritic cells (DCs) are professional antigen-presenting cells primarily responsible for acquiring, processing and presenting antigens on antigen presenting molecules to initiate T-cell-mediated immunity. Dendritic cells can be separated into several phenotypically and functionally heterogeneous subsets. Three important subsets of splenic dendritic cells are plasmacytoid, CD8&#945;Pos and CD8&#945;Neg cells. The plasmacytoid DCs are natural producers of type I interferon and are important for anti-viral T cell immunity. The CD8&#945;Neg DC subset is specialized for MHC class II antigen presentation and is centrally involved in priming CD4 T cells. The CD8&#945;Pos DCs are primarily responsible for cross-presentation of exogenous antigens and CD8 T cell priming. The CD8&#945;Pos DCs have been demonstrated to be most efficient at the presentation of glycolipid antigens by CD1d molecules to a specialized T cell population known as invariant natural killer T (iNKT) cells. Administration of Flt-3 ligand increases the frequency of migration of dendritic cell progenitors from bone marrow, ultimately resulting in expansion of dendritic cells in peripheral lymphoid organs in murine models. We have adapted this model to purify large numbers of functional dendritic cells for use in cell transfer experiments to compare in vivo proficiency of different DC subsets.

Distinct dendritic cell subsets exist as rare populations in lymphoid organs, and therefore are challenging to isolate in sufficient numbers and purity for immunological experiments. Here we describe a high efficiency, high yield method for isolation of all of the currently known major subsets of mouse splenic dendritic cells.

Fare Dendritik Hücre Alt Grupları İzolasyonunda bir Verimli ve Yüksek Verim Yöntem

Dendritic cells (DCs) are professional antigen-presenting cells primarily responsible for acquiring, processing and presenting antigens on antigen ...

Basement Membrane Matrix Encapsulated Cell Aggregation for Investigating Murine Spleen Tissue Formation

The spleen is an immune organ that plays a key role in blood-borne immune responses. The anatomical or functional loss of this tissue increases susceptibility to severe blood infections and sepsis. Auto-transplantation of spleen slices has been used clinically to replace lost tissue and restore immune function. However, the mechanism driving robust and immunologically functional spleen tissue regeneration has not been fully elucidated. Here, we aim to develop a method for aggregating and encapsulating spleen cells within a semi-solid matrix in order to investigate the cellular requirements for spleen tissue formation. Basement membrane matrix encapsulated cell constructs are amenable to both in vitro tissue culture of three-dimensional organoids as well as transplantation under the kidney capsule to directly assess in vivo tissue formation. By manipulating the input cells for aggregation and encapsulation, we demonstrate that graft-derived PDGFR&#946;+MAdCAM-1- neonatal stromal cells are required for spleen tissue regeneration under animal transplantation models.

This article describes a protocol for aggregating and encapsulating spleen cells within a semi-solid basement membrane matrix. Basement membrane matrix constructs can be used in three-dimensional culture for studying organoid development, or for in vivo transplantation and tissue regeneration studies.

Murin Dalak Dokusu Oluşumunu Araştırmak için Bazal Membran Matris Kapsüllü Hücre Agregasyonu

The spleen is an immune organ that plays a key role in blood-borne immune responses. The anatomical or functional loss of this tissue increases ...

Gelişim Biyolojisi

Isolation and Adoptive Transfer of High Salt Treated Antigen-presenting Dendritic Cells

Excess dietary salt intake contributes to inflammation and plays a vital role in the development of hypertension. We previously found that antigen-presenting dendritic cells (DCs) can sense elevated extracellular sodium leading to the activation of the NADPH oxidase and formation of isolevuglandin (IsoLG)-protein adducts. These IsoLG-protein adducts react with self-proteins and promote an autoimmune-like state and hypertension. We have developed and optimized state-of-the-art methods to study DC function in hypertension. Here, we provide a detailed protocol for isolation, in vitro treatment with elevated sodium, and adoptive transfer of murine splenic CD11c+ cells into recipient mice to study their role in hypertension.

Here, we present a protocol to isolate dendritic cells from murine spleens by magnetic cell sorting and subsequent adoptive transfer into na&#239;ve mice. The model of high-salt activated dendritic cells was chosen to explain the step-by-step procedures of adoptive transfer and flow cytometry.

Yalıtım ve yüksek tuz evlat edinen Transfer dendritik hücreler antijen sunan tedavi

Excess dietary salt intake contributes to inflammation and plays a vital role in the development of hypertension. We previously found that ...

Optimized Protocol for Mouse Wound Healing Model with Stem Cell-Derived Exosomes

Mouse Wound Models and Preparation of Single-Cell Suspensions 

The management of acute full-thickness skin injuries presents a considerable challenge in clinical practice. The complexity of wound healing, involving diverse cell populations and the intricate wound microenvironment, complicates the assessment of treatment effects and their interactions using traditional experimental approaches. Single-cell transcriptome sequencing technology has revolutionized the understanding of cellular functions and mechanisms during wound healing. However, the variability in methodologies for creating mouse wound models introduces confounding factors that can impact experimental results. Furthermore, the process of dissociating mouse skin tissues presents significant technical hurdles, highlighting the critical need for high-quality single-cell suspensions for accurate transcriptome sequencing. Therefore, this study aims to optimize the construction of mouse wound models to accurately assess changes in wound closure while eliminating variables that could influence the subsequent sequencing result. Additionally, the preparation of single-cell suspensions was performed using both enzyme preparation protocols and test kit protocols to optimize cost and effectiveness.

Mouse Wound Models and Preparation of Single-Cell Suspensions

This study presents two crucial experimental techniques: the construction of a murine wound model for assessing wound closure and the preparation of single-cell suspensions from murine skin to preserve cellular viability.

The management of acute full-thickness skin injuries presents a considerable challenge in clinical practice. The complexity of wound healing, involving ...

T-Cell Enrichment: A Technique to Isolate T-Cells from Mixed Cell Population by Magnetic Separation

Source: Nguyen, H. D., et al. <a href="https://www.jove.com/t/60083">Bone Marrow Transplantation Platform to Investigate the Role of Dendritic Cells in Graft-versus-Host Disease.</a> J. Vis. Exp. (2020).
This video describes T-cells enrichment from the spleen of a mouse by negative selection using a magnetic separator. The technique involves targeting the other cells in the sample for depletion using antibodies or ligands directed against specific cell surface antigens.

T-Hücresi Zenginleştirme: Manyetik Ayırma ile Karışık Hücre Popülasyonundan T-Hücrelerini İzole Etme Tekniği

Source: Nguyen, H. D., et al. Bone Marrow Transplantation Platform to Investigate the Role of Dendritic Cells in Graft-versus-Host Disease. J. Vis. Exp. ...

JoVE Encyclopedia of Experiments

Kanser Araştırmaları

Lösemi

Hayvan modeli çalışmaları

Isolation of Macrophages from Mouse Dorsal Root Ganglion Using Mechanical Dissociation

Source: Yu, X., et al. <a href="https://app.jove.com/v/60023">Rapid Isolation of Dorsal Root Ganglion Macrophages.</a> J. Vis. Exp. (2019)
This video demonstrates an enzyme-free protocol to isolate macrophages from mouse dorsal root ganglion (DRG) using a mechanical dissociation technique. Upon homogenization of the freshly collected DRG tissue, antibodies against the cell-surface receptors on the macrophages are used to label the cells fluorescently, followed by performing flow cytometry to confirm the presence of macrophages in the sample.

Fare Dorsal Kök Ganglionundan Makrofajların Mekanik Dissosiyasyon Kullanılarak İzolasyonu

Source: Yu, X., et al. Rapid Isolation of Dorsal Root Ganglion Macrophages. J. Vis. Exp. (2019)
This video demonstrates an enzyme-free protocol to isolate ...

Fare Dalak gentleMACS Dissociator Tek Hücreli Cezalar hazırlanması

Transkript

Özet

Daha Fazla Video Keşfet

Akım Sitometri ve Otomatik Manyetik Aktif Hücre Sıralama (AutoMACS) kullanarak Naive ve Pankreas Tümör taşıyan fareler ile myeloid türevi bastırıcı hücreler (MDSC) hazırlanması

Standardize Hazırlık gentleMACS Dissociator kullanarak Fare Akciğer Doku Tek Hücre Cezalar

Fare Sinir Doku Hücre Tek Cezalar Üretimi

Fare Naif CD4

Fare Dendritik Hücre Alt Grupları İzolasyonunda bir Verimli ve Yüksek Verim Yöntem

Murin Dalak Dokusu Oluşumunu Araştırmak için Bazal Membran Matris Kapsüllü Hücre Agregasyonu

Yalıtım ve yüksek tuz evlat edinen Transfer dendritik hücreler antijen sunan tedavi

Mouse Wound Models and Preparation of Single-Cell Suspensions

T-Hücresi Zenginleştirme: Manyetik Ayırma ile Karışık Hücre Popülasyonundan T-Hücrelerini İzole Etme Tekniği

Fare Dorsal Kök Ganglionundan Makrofajların Mekanik Dissosiyasyon Kullanılarak İzolasyonu