polarizing xenon-129 for hyperpolarized xenon spectroscopy

Hiperpolarize Ksenon-129 MRG ile Akciğer Fonksiyonunun Değerlendirilmesi

Hyperpolarized Xenon-129 (HXe) magnetic resonance imaging (MRI)1 is a technique that offers unique insights into lung structure, function, and gas exchange processes. By dramatically amplifying the magnetization of Xenon gas through spin-exchange optical pumping, HXe MRI achieves an order-of-magnitude improvement in signal-to-noise ratio compared to thermally polarized Xenon MRI2,3,4,5,6. This hyperpolarization enables the direct visualization and quantification of Xenon gas uptake into lung tissue and blood, which would otherwise be undetectable with conventional thermally polarized MRI7.
Chemical shift saturation recovery (CSSR) MR spectroscopy8,9,10,11,12,13 has proven to be one of the most valuable HXe MRI techniques. CSSR involves selectively saturating the magnetization of Xenon dissolved in lung tissue and blood using frequency-specific radiofrequency (RF) pulses. The subsequent recovery of the dissolved-phase (DP) signal as it exchanges with fresh hyperpolarized Xenon gas in the airspaces on a timescale of ms offers important functional information about the lung parenchyma.
Since its development in the early 2000s, the techniques behind CSSR spectroscopy have been progressively refined14,15,16,17,18,19,20,21,22,23. Further, advances in modeling Xenon uptake curves have enabled the extraction of specific physiological parameters, such as alveolar wall thickness and pulmonary transit times10,24,25,26. Studies have shown CSSR&#39;s sensitivity to subtle changes in lung microstructure and gas exchange efficiency in the form of pulmonary abnormalities found in clinically healthy smokers27, as well as in a range of lung diseases, including chronic obstructive pulmonary disease (COPD)18,27,28, fibrosis29, and radiation-induced lung injury30,31. CSSR spectroscopy has also been demonstrated to be sensitive to detect oscillations in the DP signal corresponding to pulsatile blood flow during the cardiac cycle32.
While significant progress has been made, practical challenges remain in implementing CSSR spectroscopy on clinical MRI systems. Scan times requiring single-dose breath holds approaching 10 s may be too long for pediatric subjects33,34 or patients with severe lung disease35,36. Additionally, the technique is susceptible to measurement biases if acquisition parameters such as the order of the saturation delay times or the efficacy of the dissolved-phase saturation are not properly optimized21. To address these limitations and make CSSR more accessible to the broader research community, clear, step-by-step protocols for both conventional breath hold and free-breathing acquisitions, currently under development, are needed.
The objective of this paper is to present a detailed methodology for performing optimized CSSR MR spectroscopy using HXe gas. The protocol will cover polarization and delivery of the Xenon gas, RF pulse calibration, sequence parameter selection, subject preparation, data acquisition, and key steps in data analysis. Examples of experimental results will be provided. It is hoped that this comprehensive guide will serve as a foundation for CSSR implementations across sites and help realize the full potential of this technique for quantifying lung microstructural changes in a range of pulmonary diseases.

Yazar Spotlight: Serbest Solunum Hiperpolarize Xenon-129 MRI ile Akciğer Hastalığı Araştırmalarını İlerletmek

Hiperpolarize Ksenon Spektroskopisinden Elde Edilen Akciğer Yapısı ve Fonksiyonunun Kantitatif Ölçümü

Hiperpolarize Ksenon Spektroskopisi için Polarize Ksenon-129

Başlamak için, çalışma başlamadan yaklaşık 2,5 saat önce ksenon polarizörü ısıtın. 250 mililitrelik özel polivinil florür veya PVF torbasının konektörünü bir tavan klipsinden geçirin. Torbayı mevcut dört polarize dağıtım portundan birine takın.Polarizör dokunmatik ekranında, zenginleştirilmiş xenon tankını seçin. Akış hızını orta ve polarizasyon hacmini 250 mililitre olarak ayarlayın. Ardından polarizasyon işlemini başlatmak için başlat düğmesine basın.Polarize ksenon gazı dağıtıldıktan sonra, polarizörün dokunmatik ekranında torbanın çıkarılması için bir mesaj görüntülenecektir. Özel PVF torbasının konektör borusunu tavan klipsi ile sıkıştırın ve ardından MRI tarayıcısının deliğine yerleştirmek için torbayı çıkarın.

polarizing-xenon-129-for-hyperpolarized-xenon-spectroscopy

Research

JoVE Journal

Medicine

Polarizing Xenon-129 for Hyperpolarized Xenon Spectroscopy

40 Görüntüleme. Başlamak için, çalışma başlamadan yaklaşık 2,5 saat önce ksenon polarizörü ısıtın. 250 mililitrelik özel polivinil florür veya PVF torbasının konektörünü bir tavan klipsinden geçirin. Torbayı mevcut dört polarize dağıtım portundan birine takın.Polarizör dokunmatik ekranında, zenginleştirilmiş xenon tankını seçin. Akış hızını orta ve polarizasyon hacmini 250 mililitre olarak ayarlayın. Ardından polarizasyon işlemini başlatmak için başlat dü...

Video: Hiperpolarize Ksenon Spektroskopisi için Polarize Ksenon-129

Quantitative Measure of Lung Structure and Function Obtained from Hyperpolarized Xenon Spectroscopy

Hyperpolarized Xenon-129 (HXe) magnetic resonance imaging (MRI) provides tools for obtaining 2- or 3-dimensional maps of lung ventilation patterns, gas diffusion, Xenon uptake by lung parenchyma, and other lung function metrics. However, by trading spatial for temporal resolution, it also enables tracing of pulmonary Xenon gas exchange on a ms timescale. This article describes one such technique, chemical shift saturation recovery (CSSR) MR spectroscopy. It illustrates how it can be used to assess capillary blood volume, septal wall thickness, and the surface-to-volume ratio in the alveoli. The flip angle of the applied radiofrequency pulses (RF) was carefully calibrated. Single-dose breath-hold and multi-dose free-breathing protocols were employed for administering the gas to the subject. Once the inhaled Xenon gas reached the alveoli, a series of 90&#176; RF pulses was applied to ensure maximum saturation of the accumulated Xenon magnetization in the lung parenchyma. Following a variable delay time, spectra were acquired to quantify the regrowth of the Xenon signal due to gas exchange between the alveolar gas volume and the tissue compartments of the lung. These spectra were then analyzed by fitting complex pseudo-Voigt functions to the three dominant peaks. Finally, the delay time-dependent peak amplitudes were fitted to a one-dimensional analytical gas-exchange model to extract physiological parameters.

The manuscript presents a detailed protocol for using hyperpolarized Xenon-129 chemical shift saturation recovery (CSSR) to trace pulmonary gas exchange, assess the apparent alveolar septal wall thickness, and measure the surface-to-volume ratio. The method has the potential to diagnose and monitor lung diseases.

Hyperpolarized Xenon-129 (HXe) magnetic resonance imaging (MRI) provides tools for obtaining 2- or 3-dimensional maps of lung ventilation patterns, gas ...