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Closed Circular Extrachromosomal Ribosomal DNA (rDNA) Containing Element (CERE) Isolation from Transfected Naegleria Gruberi Trophozoites and PCR Analysis

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02:27 min

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June 21st, 2024

DOI :

10.3791/202099-v

June 21st, 2024

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Brian T. Nguyen¹, Nora M. Chapman², John C. Mullican³, Kristen M. Drescher¹

¹Department of Medical Microbiology and Immunology, Creighton University School of Medicine, ²Department of Pathology and Microbiology, University of Nebraska Medical Center, ³Department of Biology, Washburn University

Transkript

Daha Fazla Video Keşfet

Closed Circular Extrachromosomal Ribosomal DNA

Naegleria Gruberi

Sodium Chloride

Ammonium Acetate

Absolute Ethanol

Gel Electrophoresis

Ultraviolet Light System

Plasmid Retention

Seriden

Using a CERE-Based Vector for Transfection of Naegleria gruberi Trophozoites

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