Washburn University
2 ARTICLES PUBLISHED IN JoVE
Environment
Reliable Method for Assessing Seed Germination, Dormancy, and Mortality under Field Conditions
Brian A. Pace 1, Helen M. Alexander 2, D. Jason Emry 3, Kristin L. Mercer 1
1Department of Horticulture and Crop Science, Ohio State University, 2Department of Ecology and Evolutionary Biology, University of Kansas, 3Department of Biology, Washburn University
Here we present a protocol for assessing seed survivorship, germination and dormancy under field conditions using buried, labeled seed strips and tetrazolium chloride (TZ) viability testing.
Biology
Transfection of a Molecular Clone of Naegleria gruberi rDNA into N. gruberi Trophozoites
Brian T. Nguyen 1, Nora M. Chapman 2, John C. Mullican 3, Kristen M. Drescher 1
1Department of Medical Microbiology and Immunology, Creighton University School of Medicine, 2Department of Pathology and Microbiology, University of Nebraska Medical Center, 3Department of Biology, Washburn University
This protocol describes a methodology to transfect Naegleria gruberi trophozoites with a construct that is maintained throughout passaging trophozoites in vitro, as well as through encystment and excystment.
JoVE Hakkında
Telif Hakkı © 2020 MyJove Corporation. Tüm hakları saklıdır