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Absorbance-Based Microplate Analysis to Test the Serum Stability of Human Adenosine Deaminase 1

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02:28 min

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September 27th, 2024

DOI :

10.3791/202214-v

September 27th, 2024

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Yusef Haikal¹, Maria Rain Jennings², Johnathan Nguyen¹, John J. Blazeck¹

¹School of Chemical and Biomolecular Engineering, Georgia Institute of Technology, ²Johns Hopkins University School of Medicine

Transkript

Daha Fazla Video Keşfet

Absorbance based Analysis

Microplate Reader

Serum Stability

Human Adenosine Deaminase 1

Adenosine Stock Preparation

Diluted Protein Samples

Absorbance Measurement

Slope Determination

Fraction Of Activity Remaining

Wildtype HsADA1 Activity

Absorbance Decline Curves

Negative Control Samples

Seriden

Evaluating Serum Stability of HsADA1: A Microplate-Based Approach

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