Immunoprecipitation of RapR-Shp2 to Study Chemogenetic Control of Tyrosine Phosphatase

To begin, culture and prepare the HEK 293 T-cells expressing RapR-Shp2 for immunoprecipitation. For preparing the Sepharose, add resuspended Protein G-Sepharose into a 1.5 milliliter tube. After adding the lysis buffer, micro-centrifuge the tube f