The overall goal of this procedure is to assess individual CFTR function in an efficacy of CFTR modulating treatments using a forskolin-induced swelling, or FIS assay, in intestinal organoids generated from Cystic Fibrosis patients. This method ad
Bu içeriğe erişmek için giriş yapın veya ücretsiz denemenizi başlatın
This protocol describes an assay for measuring CFTR function and CFTR modulator responses in cultured tissue from subjects with cystic fibrosis (CF). Biopsy-derived intestinal organoids swell in a cAMP-driven fashion, a response that is defective (or strongly reduced) in CF organoids and can be restored by exposure to CFTR modulators.