Fabrication of Three-dimensional Paper-based Microfluidic Devices for Immunoassays

0:57

Preparation of Paper Layers and Nylon Membrane Layer

2:51

Creating Hydrophobic Barriers in the Printed Layer

3:53

Preparation of Adhesive Layers and Bqacking of Device Layers with Adhesive

4:52

Treatment of Conjugate Layer with Reagents for Immunoassays Prior to Device Assembly

5:38

Treatment of Lateral Channel and Capture Layer with Reagents for Immunoassays Prior to Device Assembly

6:52

Assembly of Three-dimensional Paper-based Microfluidic Devices

8:33

Performing a Paper-based Immunoassay

9:17

Results: Fabrication of Paper-based Immunoassays

10:33

Conclusion