Single cell sort and reverse transcription reaction
3:37
Multiplex-nested PCR
8:56
Subcloning TCR alpha and beta chains
9:56
Verification of TCR expression
10:46
Conclusion
Transkript
This method can help answer key questions in the immunology field, such as the TCR alpha and beta repertoire of an antigen's specific population, as well as the in vivo functionality of human TCRs. The main advantages of this technique are the rel
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The current protocol combines single cell paired human TCR alpha and beta chain sequencing with streamlined generation of retroviral vectors compatible with in vitro and in vivo TCR expression.