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Baylor College of Medicine, Texas Children's Hospital

2 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

Retroviral Transduction of Bone Marrow Progenitor Cells to Generate T-cell Receptor Retrogenic Mice

Thomas Lee¹, Ivan Shevchenko¹, Maran L. Sprouse¹, Maria Bettini¹, Matthew L. Bettini¹

¹Department of Pediatrics, Baylor College of Medicine

We present a rapid and flexible protocol for a single T cell receptor (TCR) retroviral-based in vivo expression system. Retroviral vectors are used to transduce bone marrow progenitor cells to study T cell development and function of a single TCR in vivo as an alternative to TCR transgenic mice.

Immunology and Infection

Streamlined Single Cell TCR Isolation and Generation of Retroviral Vectors for In Vitro and In Vivo Expression of Human TCRs

Maran L. Sprouse¹, Gabriele Blahnik², Thomas Lee¹, Natalie Tully¹, Pinaki Benarjee³, Eddie A. James², Maria J. Redondo⁴, Matthew L. Bettini¹, Maria Bettini¹

¹Department of Pediatrics, Section of Diabetes and Endocrinology, McNair Medical Institute, Baylor College of Medicine, Texas Children's Hospital, ²Benaroya Research Institute at Virginia Mason, ³Center for Human Immunobiology, Baylor College of Medicine, Texas Children's Hospital, ⁴Department of Pediatrics, Section of Diabetes and Endocrinology, Baylor College of Medicine, Texas Children's Hospital

The current protocol combines single cell paired human TCR alpha and beta chain sequencing with streamlined generation of retroviral vectors compatible with in vitro and in vivo TCR expression.