Preparation of Potassium Ion-selective Microelectrodes
4:18
Calibration of Potassium Ion-selective Microelectrodes
5:40
Acute Slice Preparation
7:16
Measurement of Electrically Evoked K Release
9:26
Results: Calibration of Microelectrodes and Measurement of Electrically Evoked K Release
10:53
Conclusion
Transkript
The overall goal of this technique is to make, calibrate, and use monopolar potassium ion-selective microelectrodes. Using such electrodes enables the quantitative assessment of evoked potassium ion concentration dynamics in adult brain slices. Th
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Potassium ions contribute to the resting membrane potential of cells and extracellular K+ concentration is a crucial regulator of cellular excitability. We describe how to make, calibrate and use monopolar K+-selective microelectrodes. Using such electrodes enables the measurement of electrically evoked K+ concentration dynamics in adult hippocampal slices.