Fetal Tibia and Femur Culture and Full Bone Length and Mineralized Region Analysis
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Results: Representative Total Bone Length Growth and Mineralized Tibia Measurement and Analysis
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Conclusion
Transkript
This method adapts the metatarsal culture protocol to bigger bones, allowing direct bone manipulation to be combined with complex genetic models to address processes related to bone development. This technique allows manipulation and analysis of b
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Here, we present a method for ex vivo culture of long murine bones at both fetal and newborn stages, suitable for analyzing bone and cartilage development and homeostasis in controlled conditions while recapitulating the in vivo process.