Sphere Formation and Derived Adherent Population Generation
2:28
Sphere-forming Capacity Determination
3:02
Self-renewal Determination
3:59
Sphere Projection Area Determination
4:51
Results: Representative Endometrial and Breast Cancer Cell Sphere and Derived Adherent Population Analyses
6:29
Conclusion
Transkript
This protocol allows the identification and isolation of cancer stem cells in a robust manner using functional assays and phenotypic characterization. Cancer stem cell isolation from a tumor sample could be a platform for guiding the clinical appl
Bu içeriğe erişmek için giriş yapın veya ücretsiz denemenizi başlatın
The aim of this methodology is to identify cancer stem cells (CSC) in cancer cell lines and primary human tumor samples with the sphere-forming protocol, in a robust manner, using functional assays and phenotypic characterization with flow cytometry and Western blot.