Sign In

Nutrients in Aquatic Ecosystems

Overview

Source: Laboratories of Margaret Workman and Kimberly Frye - Depaul University

Nitrogen and phosphorus are essential plant nutrients found in aquatic ecosystems and both are monitored as a part of water quality testing because in excess amounts they can cause significant water quality problems. 

Nitrogen in water is measured as the common form nitrate (NO3-) that is dissolved in water and readily absorbed by photosynthesizers such as algae. The common form of phosphorus measured is phosphate (PO43-), which is strongly attracted to sediment particles as well as dissolved in water. In excess amounts, both nutrients can cause an increase in aquatic plant growth (algal bloom, Figure 1) that can disrupt the light, temperature, and oxygen levels in the water below and lead to eutrophication and hypoxia (low dissolved oxygen in water) forming a “dead zone” of no biological activity. Sources of nitrates and phosphorus include wastewater treatment plants, runoff from fertilized lawns and agricultural lands, faulty septic systems, animal manure runoff, and industrial waste discharge.

Figure 1
Figure 1. Algal bloom
Taken in 2011, the green scum shown in this image was the worst algae bloom Lake Erie has experienced in decades. Record torrential spring rains washed fertilizer into the lake, promoting the growth of microcystin producing cyanobacteria blooms. Vibrant green filaments extend out from the northern shore.

Procedure

1. Measure Nitrogen in Sample

  1. On the spectrophotometer, find the program for nitrate (with user manual or instrument menu) and enter the program number.  
  2. Pipette 10 mL of the water sample into one of the sample tubes. Pour this into one of the sample tubes.
  3. Repeat for a second sample tube.
  4. Add the contents of one nitrate reagent powder pillow to one sample tube.
  5. Cap both sample tubes.
  6. On the spectrophotometer, press timer and enter to start a r

Log in or to access full content. Learn more about your institution’s access to JoVE content here

Results

Figure 2
Figure 2. Graph comparing nitrates between different land use types (undeveloped, agricultural, and urban).

Average nitrate concentrations compared upstream and downstream from a water treatment plant (Figure 3). The downstream measurement represents the discharge from the treatment.

Log in or to access full content. Learn more about your institution’s access to JoVE content here

Application and Summary

High concentrations of nitrates and phosphorus can stimulate eutrophic conditions in water by causing algal bloom that negatively affects other water quality factors including dissolved oxygen, temperature, and other indicators. Excess nitrates can lead to hypoxic water (low levels of dissolved oxygen) no longer able to support aerobic life creating a “dead zone,” where non-mobile species mass die-offs and mobile species move away to other waters. Dead zones are occurring globally in coastal regions where lar

Log in or to access full content. Learn more about your institution’s access to JoVE content here

Tags
NutrientsAquatic EcosystemsNitrogenPhosphorusWater Quality ProblemsNitratePhosphatePhotosynthesizersAlgaeFreshwater RunoffWastewater Treatment PlantsFertilized LawnsAgricultural LandsSeptic SystemsIndustrial Waste DischargeEutrophicationAlgae BloomsHypoxiaDead ZoneFish MortalityUrban AreasMeasuring Nitrate And Phosphate ConcentrationsSurface Water

Skip to...

0:00

Overview

1:40

Principles of Nitrate and Phosphate Detection

3:40

Measuring Nitrogen in a Sample

5:11

Measuring Phosphorus in a Sample

7:11

Results

8:29

Applications

10:25

Summary

Videos from this collection:

article

Now Playing

Nutrients in Aquatic Ecosystems

Environmental Science

38.2K Views

article

Tree Identification: How To Use a Dichotomous Key

Environmental Science

79.0K Views

article

Tree Survey: Point-Centered Quarter Sampling Method

Environmental Science

48.9K Views

article

Using GIS to Investigate Urban Forestry

Environmental Science

11.8K Views

article

Proton Exchange Membrane Fuel Cells

Environmental Science

21.8K Views

article

Biofuels: Producing Ethanol from Cellulosic Material

Environmental Science

51.7K Views

article

Testing For Genetically Modified Foods

Environmental Science

88.3K Views

article

Turbidity and Total Solids in Surface Water

Environmental Science

35.4K Views

article

Dissolved Oxygen in Surface Water

Environmental Science

54.9K Views

article

Measuring Tropospheric Ozone

Environmental Science

26.0K Views

article

Determination Of NO<sub>x</sub> in Automobile Exhaust Using UV-VIS Spectroscopy

Environmental Science

29.6K Views

article

Lead Analysis of Soil Using Atomic Absorption Spectroscopy

Environmental Science

123.8K Views

article

Carbon and Nitrogen Analysis of Environmental Samples

Environmental Science

28.8K Views

article

Soil Nutrient Analysis: Nitrogen, Phosphorus, and Potassium

Environmental Science

213.9K Views

article

Analysis of Earthworm Populations in Soil

Environmental Science

16.3K Views

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2025 MyJoVE Corporation. All rights reserved