JoVE Logo
Faculty Resource Center

Sign In

0:00

Overview

1:06

Considerations for Identification Methods

3:40

Tail Tattooing in Adults

6:24

Toe Tattooing in Neonates and Adults

9:16

Microchip Placement

10:24

Method for Temporary Identification

11:21

Applications

13:00

Summary

啮齿动物鉴定 (2)

来源: 凯斯图尔特, RVT, RLATG, CMAR;瓦莱丽. 施罗德, RVT, RLATG。圣母大学, 在

动物记录必须得到准确的维护, 以确保数据收集是正确的。记录的范围从保持笼卡的信息, 以拥有一个详细的数据库与所有有关的信息, 每个动物。记录记录的主要组成部分是研究动物的个体识别。有多种方法可用于鉴别小鼠和大鼠。本视频描述了纹身、微芯片放置和临时识别方法的程序技术, 并探讨了每个步骤的优点.

1. 尾部纹身

尾部纹身很容易阅读, 不需要处理动物.

Figure 1
图1。成年老鼠的尾巴纹身

  1. 根据制造商和 #39 的说明准备纹身设备。少量墨水是必需的。黑染料应用于白化小鼠或大鼠, 绿色染料的色素小鼠或大鼠.
  2. 为识别准备动物.
    注意: 为了尽量减?...

Log in or to access full content. Learn more about your institution’s access to JoVE content here

在选择适当的识别方法时, 必须慎重考虑许多因素。每种技术都有其优缺点, 必须在实验需要方面加以考虑。虽然成本必须与其他因素一起权衡, 但技术的简单性和对动物的不适程度应该是首要考虑。 45

Log in or to access full content. Learn more about your institution’s access to JoVE content here

  1. Robinson, V., Morton, D.B., Anderson, D., Carver, J.F.A., Francis, R.J., Hubrecht, R., Jenkins, E., Mathers, K.E., Raymond, R., Rosewell, I., Wallace, J., and Wells, D.J. 2003. Refinement and reduction in production of genetically modified mice. Laboratory Animals. 37:S1-S50.
  2. Schaefer, D.C., Asner, I.N., Seifert, B., Bürki, K., and Cinelli, P. 2010. Analysis of physiological and behavioural parameters in mice after toe clipping as newborns. Laboratory Animals. 44:7-13
  3. Castelhano-Carlos, M.J., Sousa, N., Ohl, F., and Baumans, V. 2010. Identification methods in newborn C57BL/6 mice: a developmental and behavioural evaluation. Laboratory Animals. 44: 88-103.
  4. Danneman, P.J., Suckow, M.A., and Brayton, C.F. 2013. The laboratory mouse. Second edition. New York, NY: CRC Press.
  5. Institute for the Laboratory Animal Research. 2011. Guide for the care and use of laboratory animals, 8th ed. Washington (DC): National Academies Press.

-- Views

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved