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Overview

1:09

Considerations for the Specialized Injections

4:46

Intradermal Administration

6:30

Intranasal Administration

7:59

Intracranial Administration in Neonatal Rodents

10:38

Applications

11:56

Summary

药物注射 III

来源: 凯斯图尔特, RVT, RLATG, CMAR;瓦莱丽. 施罗德, RVT, RLATG。圣母大学, 在

在实验室小鼠和大鼠中有许多常用的复合用药路线。然而, 某些协议可能需要使用不太常用的路线, 包括皮内、鼻腔和颅内注射。专业培训对于这些程序的成功执行至关重要。为获得机构动物保育和使用委员会 (IACUC) 批准, 可能需要提供这些路线的理由。

1. 皮内管理

  1. 大多数皮内注射是基化合物。解决方案必须在生理学上进行缓冲, 以获得中性的 pH 值, 以避免注射部位出现组织坏死.
  2. 针的大小范围是25-30 计, 最小的可能.
  3. 每个注射场的剂量范围是50-100 和 #181; 注射过量可能导致注射部位坏死, 或由于压力而在现场漏出该化合物.
  4. 为了准确地将针放置到皮内空间, 必须对小鼠和老鼠进行麻醉。吸入麻醉可以快速诱导和...

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对动物的化合物进行管理可以对动物的福祉和实验数据的结果和科学价值产生重大影响。正确的分娩方式对实验的成功至关重要。必须考虑许多因素来确定最佳路线, 包括研究的科学目的、物质的 pH 值、所需的剂量量、物质的黏度以及动物的福祉。技术专长也是所有注塑方法的要求.

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  1. Turner, P.V., Pekow, C., Vasbinder, M. A., and Brabb, T. 2011. Administration of substances to laboratory animals: equipment and considerations, vehicle selection, and solution preparation. JAALAS. 50: 614-627.
  2. Dhuria, S.V., Hanson, L.R., and Frey II, W.H. 2010. Intranasal delivery to the central nervous system: mechanisms and experimental considerations. Journal of Pharmaceutical Sciences. 99: 1654-1673.
  3. Stevens, J., Suidgeest, E, Van der Graaf, P.H., Danhof, M., and De Lange, E.C. 2008. Development and evaluation of a new, minimal-stress animal model for intranasal administration in freely moving rats. Poster presentation at American Association of Pharmaceutical Scientists Annual Meeting, Atlanta, Georgia.
  4. Morton, D.A., Jennings, M., Buckwell, A., Ewbank, R., Godfrey, C., Holgate, B., Inglis, I., James, R., Page, C., Sharman, I., Verschoyle, R., Westall, L., and Wilson, A.B. 2001. Refining procedures for the administration of substances Report of the BVAAWF/FRAME/RSPCA/UFAW Joint Working Group on Refinement. Members of the Joint Working Group on Refinement. Laboratory Animals. 35: 1-41.

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