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0:00

Overview

0:50

Preparatory Steps

2:53

External and Internal Gross Examination

7:36

Abdominal Organs

12:04

Reproductive System

14:35

Thoracic Organs

16:59

Applications

18:23

Summary

诊断尸检和组织采集

来源: 凯斯图尔特, RVT, RLATG, CMAR;瓦莱丽. 施罗德, RVT, RLATG。圣母大学, 在

许多动物实验依赖于收集和检测器官和组织的最终数据收集时间点。使用适当的方法收集器官和组织可以影响样品的质量和分析为组织的测试收集的数据。这种动物的安乐死方法也会影响样品的质量。这份手稿将概述适当的尸检技术的大鼠.

1. 外部检查

对身体进行肉眼检查, 包括对身体进行病灶和肿块的目测, 应作为尸检的初始步骤进行。头发脱落的部位应检查毛发。牙齿和指甲被评估为过度增长或磨损。任何染色的毛皮在口腔, 鼻孔, 眼睛, 肛门, 和生殖器的开口应该注意。应进行磁带测试、皮肤刮擦和毛皮检查, 以检测外部寄生虫 (见下面的步骤).

磁带测试所需的
  1. 磁带测试
    1. 设备包括手套、透...

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许多研究项目的最后一步是实验动物的尸检。详细观察外部和内部结构, 然后收集组织进行进一步分析, 提供了大量的研究数据。适当的方法, 组织去除和保存适当的固定液是必不可少的正确解释的结果.

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  1. Leary, S., Underwood, W., Anthony, R., Cartner, S., Corey, D., Grandin, T., et al. 2013. AVMA guidelines for the euthanasia of animals: 2013 edition.
  2. Parkinson, C.M., O'Brien, A., Albers, T.M., Simon, M.A., Clifford, C.B. and Pritchett-Corning, K.R. 2011. Diagnostic Necropsy and Selected Tissue and Sample Collection in Rats and Mice. 54. e2966. 1-7.
  3. Fiette, L. and Slaoui, M. 2011. Necropsy and Sampling Procedures in Rodents. Drug Safety Evaluation: Methods and Protocols, 39-67.
  4. Youngson, R.M. 2005. Collins dictionary of Medicine fourth edition.

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