Name: use of rotorod as a method for the qualitative analysis of walking in rat
Uploaded: 2008-12-10T00:00:00
Description: Watch this Scientific Journal Video about Use of Rotorod as a Method for the Qualitative Analysis of Walking in Rat at JoVE.com

स्वास्थ्य अनुसंधान, कनाडा स्ट्रोक नेटवर्क, और चिकित्सा अनुसंधान के लिए अलबर्टा विरासत फाउंडेशन के कनाडा के संस्थानों से अनुदान द्वारा समर्थित है.

Rotorod चलने तंत्र rotorod एक rotating सिलेंडर, व्यास में 4 सेमी, जमीन के ऊपर 35 सेमी तय की और पारदर्शी Plexiglas द्वारा संलग्न किया गया था. rotorod सिलेंडर textured रबर कोटिंग है, जो कर्षण सुविधा में शामिल किया गया था. तंत्र के फ?...

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वर्तमान अध्ययन का उद्देश्य जांच करने के लिए कि क्या कृंतक हरकत कौशल के लिए rotorod परीक्षण मुद्रा के गुणात्मक परीक्षा forelimb निकलते हैं और हिंद अंग तंत्रिका तंत्र घायल चूहों में कदम के लिए इस्तेमाल किया जा सकता था. वि?...

A correction was made to: <a href="http://www.jove.com/video/1030/">Use of Rotorod as a Method for the Qualitative Analysis of Walking in Rat</a>. An additional paragraph was added to the discussion, the abstract was modified, and another reference was included.
Additional reference:
15. Whishaw IQ, Li K, Whishaw PA, Gorny B, Metz GA. Distinct forelimb and hind limb stepping impairments in unilateral dopamine-depleted rats: use of the rotorod as a method for the qualitative analysis of skilled walking. J Neurosci Meth 126, 13-23 (2003).
Revised Abstract:
High speed videoanalysis of the details of movement can provide a source of information about qualitative aspects of walking movements. When walking on a rotorod, animals remain in approximately the same place making repetitive movements of stepping. Thus the task provides a rich source of information on the details of foot stepping movements. Subjects were hemi-Parkinson analogue rats, produced by injection of 6-hydroxydopamine (6-OHDA) into the right nigrostriatal bundle to deplete nigrostriatal dopamine (DA). The present report provides a video analysis illustration of animals previously were filmed from frontal, lateral, and posterior views as they walked (15). Rating scales and frame-by-frame replay of the video records of stepping behavior indicated that the hemi-Parkinson rats were chronically impaired in posture and limb use contralateral to the DA-depletion. The contralateral limbs participated less in initiating and sustaining propulsion than the ipsilateral limbs. These deficits secondary to unilateral DA-depletion show that the rotorod provides a use task for the analysis of stepping movements.
Additional Discussion Paragraph:
A more detailed presentation of the present study has been made (Whishaw et al, 2003), but the present study presents the video support describing the stepping movement in the good and affected limbs of unilateral dopamine-depleted rats. For the analysis, rats with unilateral DA depletions and control rats were video recorded from front, lateral and posterior views. A rating scale of posture and forelimb movements indicated that stepping movements were chronically impaired following surgery. Examination of limb movements indicated that whereas the DA-depleted rats could use the limbs contralateral to the lesion for support, they received minimal use for shifting weight. The results of this study indicate that the rotorod task, in addition to providing quantitative measures of motor impairments, can also provide insights into the qualitative impairments.
Original Abstract:
The rotorod test, in which animals walk on a rotating drum, is widely used to assess motor status in laboratory rodents. Performance is measured by the duration that an animal stays up on the drum as a function of drum speed. Here we report that the task provides a rich source of information about qualitative aspects of walking movements. Because movements are performed in a fixed location, they can readily be examined using high-speed video recording methods. The present study was undertaken to examine the potential of the rotorod to reveal qualitative changes in the walking movements of hemi-Parkinson analogue rats, produced by injection of 6-hydroxydopamine (6-OHDA) into the right nigrostriatal bundle to deplete nigrostriatal dopamine (DA). Beginning on the day following surgery and then periodically over the next two months, the rats were filmed from frontal, lateral, and posterior views as they walked on the rotorod. Behavior was analyzed by frame-by-frame replay of the video records. Rating scales of stepping behavior indicated that the hemi-Parkinson rats were chronically impaired in their posture and in the use of the limbs contralateral to the DA-depletion. The contralateral limbs not only displayed postural and movement abnormalities, they participated less in initiating and sustaining propulsion than did the ipsilateral limbs. These findings not only reveal new deficits secondary to unilateral DA-depletion, but also show that the rotorod can provide a robust tool for the qualitative analysis of movement.

A correction was made to: <a href="http://www.jove.com/video/1030/">Use of Rotorod as a Method for the Qualitative Analysis of Walking in Rat</a>. An additional paragraph was added to the discussion, the abstract was modified and another reference was included.

Erratum: Use of Rotorod as a Method for the Qualitative Analysis of Walking in Rat

use of rotorod as a method for the qualitative analysis of walking in rat

rotorod परीक्षण, जिसमें पशुओं के एक घूर्णन ड्रम पर चलना व्यापक रूप से प्रयोगशाला कृन्तकों में मोटर स्थिति का आकलन करने के लिए प्रयोग किया जाता है. प्रदर्शन अवधि कि एक जानवर ड्रम पर ड्रम गति के एक समारोह के रूप में रहता है से मापा जाता है. यहाँ हम रिपोर्ट है कि कार्य चलने के आंदोलनों के गुणात्मक पहलुओं के बारे में में जानकारी का एक समृद्ध स्रोत प्रदान करता है. क्योंकि आंदोलनों को एक निश्चित स्थान में प्रदर्शन कर रहे हैं, वे आसानी से उच्च गति वीडियो रिकॉर्डिंग तरीकों का उपयोग कर जांच किया जा सकता है. वर्तमान अध्ययन rotorod की क्षमता HEMI पार्किंसंस अनुरूप चूहों, सही nigrostriatal बंडल में hydroxydopamine 6 (6 OHDA) के इंजेक्शन द्वारा उत्पादित के चलने आंदोलनों में गुणात्मक परिवर्तन प्रकट करने के लिए nigrostriatal डोपामाइन खलाना (जांच किया गया डीए ). दिन सर्जरी के बाद की शुरुआत तो अगले दो महीनों से अधिक समय समय पर, चूहों ललाट, पार्श्व, और पीछे के विचार से फिल्माया गया के रूप में वे rotorod पर चला गया. इस व्यवहार में फ्रेम दर फ्रेम द्वारा वीडियो कैसेट की पुनरावृत्ति विश्लेषण किया गया था. व्यवहार कदम की रेटिंग तराजू संकेत दिया है कि Hemi - पार्किंसंस चूहों लंबे समय से उनकी मुद्रा में और महंगाई भत्ते रिक्तीकरण contralateral अंग के उपयोग में बिगड़ा थे. contralateral अंग postural और आंदोलन असामान्यताएं ही प्रदर्शित नहीं है, वे की शुरुआत और बनाए रखने प्रणोदन से ipsilateral अंग किया में कम भाग लिया. इन निष्कर्षों को न केवल नए डीए रिक्तीकरण एकतरफा माध्यमिक घाटे पता चलता है, लेकिन यह भी पता चलता है कि rotorod आंदोलन के गुणात्मक विश्लेषण के लिए एक मजबूत उपकरण प्रदान कर सकते हैं.

Watch this Scientific Journal Video about Use of Rotorod as a Method for the Qualitative Analysis of Walking in Rat at JoVE.com

Use of Rotorod as a Method for the Qualitative Analysis of Walking in Rat

rotorod परीक्षण Hemi - पार्किंसंस अनुरूप चूहों के चलने आंदोलन में मोटर स्थिति का आकलन करने के लिए प्रयोग किया जाता है.

के गुणात्मक विश्लेषण के लिए एक विधि चूहा में चलना के रूप में Rotorod का प्रयोग

High speed videoanalysis of the details of movement can provide a source of information about qualitative aspects of walking movements. When walking on a ...

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