Name: use of rotorod as a method for the qualitative analysis of walking in rat
Uploaded: 2008-12-10T00:00:00
Description: Watch this Scientific Journal Video about Use of Rotorod as a Method for the Qualitative Analysis of Walking in Rat at JoVE.com

Apoiado por subvenções dos Institutos Canadenses de Pesquisa em Saúde, a Rede canadense Stroke, e os Alberta Heritage Foundation for Medical Research.

Rotorod aparelho andando O rotorod era um cilindro rotativo, 4 cm de diâmetro, 35 centímetros fixo acima do solo e cercado por acrílico transparente. O cilindro rotorod estava coberto de revestimento de borracha texturizada, o que facilita a tração. O piso do aparelho foi coberto com uma camada de espuma, para evitar lesões quando os animais caíram. Um pequeno motor elétrico desde o poder para transformar o rotorod através de uma correia de borracha. O rotorod foi criado para rodar uma...

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O objetivo do presente estudo foi examinar se o teste para a habilidade rotorod locomoção de roedores pode ser usado para a análise qualitativa da postura, pisando forelimb e dos membros posteriores em ratos pisar sistema nervoso lesionado. Para a análise, ratos com depleção DA unilateral e ratos controle foram de vídeo gravado a partir de vistas frontal, lateral e posterior. A escala de classificação da postura e movimentos forelimb indicou que os movimentos estavam pisando cronicamente prejudicada após a cirurgia. Exame dos movi...

A correction was made to: <a href="http://www.jove.com/video/1030/">Use of Rotorod as a Method for the Qualitative Analysis of Walking in Rat</a>. An additional paragraph was added to the discussion, the abstract was modified, and another reference was included.
Additional reference:
15. Whishaw IQ, Li K, Whishaw PA, Gorny B, Metz GA. Distinct forelimb and hind limb stepping impairments in unilateral dopamine-depleted rats: use of the rotorod as a method for the qualitative analysis of skilled walking. J Neurosci Meth 126, 13-23 (2003).
Revised Abstract:
High speed videoanalysis of the details of movement can provide a source of information about qualitative aspects of walking movements. When walking on a rotorod, animals remain in approximately the same place making repetitive movements of stepping. Thus the task provides a rich source of information on the details of foot stepping movements. Subjects were hemi-Parkinson analogue rats, produced by injection of 6-hydroxydopamine (6-OHDA) into the right nigrostriatal bundle to deplete nigrostriatal dopamine (DA). The present report provides a video analysis illustration of animals previously were filmed from frontal, lateral, and posterior views as they walked (15). Rating scales and frame-by-frame replay of the video records of stepping behavior indicated that the hemi-Parkinson rats were chronically impaired in posture and limb use contralateral to the DA-depletion. The contralateral limbs participated less in initiating and sustaining propulsion than the ipsilateral limbs. These deficits secondary to unilateral DA-depletion show that the rotorod provides a use task for the analysis of stepping movements.
Additional Discussion Paragraph:
A more detailed presentation of the present study has been made (Whishaw et al, 2003), but the present study presents the video support describing the stepping movement in the good and affected limbs of unilateral dopamine-depleted rats. For the analysis, rats with unilateral DA depletions and control rats were video recorded from front, lateral and posterior views. A rating scale of posture and forelimb movements indicated that stepping movements were chronically impaired following surgery. Examination of limb movements indicated that whereas the DA-depleted rats could use the limbs contralateral to the lesion for support, they received minimal use for shifting weight. The results of this study indicate that the rotorod task, in addition to providing quantitative measures of motor impairments, can also provide insights into the qualitative impairments.
Original Abstract:
The rotorod test, in which animals walk on a rotating drum, is widely used to assess motor status in laboratory rodents. Performance is measured by the duration that an animal stays up on the drum as a function of drum speed. Here we report that the task provides a rich source of information about qualitative aspects of walking movements. Because movements are performed in a fixed location, they can readily be examined using high-speed video recording methods. The present study was undertaken to examine the potential of the rotorod to reveal qualitative changes in the walking movements of hemi-Parkinson analogue rats, produced by injection of 6-hydroxydopamine (6-OHDA) into the right nigrostriatal bundle to deplete nigrostriatal dopamine (DA). Beginning on the day following surgery and then periodically over the next two months, the rats were filmed from frontal, lateral, and posterior views as they walked on the rotorod. Behavior was analyzed by frame-by-frame replay of the video records. Rating scales of stepping behavior indicated that the hemi-Parkinson rats were chronically impaired in their posture and in the use of the limbs contralateral to the DA-depletion. The contralateral limbs not only displayed postural and movement abnormalities, they participated less in initiating and sustaining propulsion than did the ipsilateral limbs. These findings not only reveal new deficits secondary to unilateral DA-depletion, but also show that the rotorod can provide a robust tool for the qualitative analysis of movement.

A correction was made to: <a href="http://www.jove.com/video/1030/">Use of Rotorod as a Method for the Qualitative Analysis of Walking in Rat</a>. An additional paragraph was added to the discussion, the abstract was modified and another reference was included.

Erratum: Use of Rotorod as a Method for the Qualitative Analysis of Walking in Rat

use of rotorod as a method for the qualitative analysis of walking in rat

O teste rotorod, em que os animais andar sobre um tambor rotativo, é amplamente utilizado para avaliar o estado do motor em roedores de laboratório. O desempenho é medido pela duração que um animal permanece em cima do tambor, em função da velocidade do tambor. Aqui nós relatamos que a tarefa fornece uma rica fonte de informações sobre aspectos qualitativos dos movimentos a pé. Porque os movimentos são realizados em um local fixo, eles podem ser facilmente analisados ​​usando métodos de alta velocidade de gravação de vídeo. O presente estudo foi realizado para examinar o potencial do rotorod para revelar mudanças qualitativas nos movimentos andando de ratos hemi-Parkinson analógico, produzido por injeção de 6 hidroxidopamina (6-OHDA) no feixe direito nigroestriatal de dopamina nigroestriatal a esgotar (DA ). Início no dia seguinte a cirurgia e depois periodicamente ao longo dos próximos dois meses, os ratos foram filmados a partir de vistas frontal, lateral e posterior, enquanto caminhavam no rotorod. Comportamento foi analisado pelo quadro a quadro-repetição dos registros de vídeo. Escalas de avaliação de pisar comportamento indicou que os ratos hemi-Parkinson foram cronicamente prejudicada em sua postura e no uso dos membros contralateral à depleção de DA. Os membros contralateral não apenas disponibilizados alterações posturais e de movimento, que participaram menos de iniciar e manter a propulsão do que os membros ipsilateral. Estes resultados não só revelam déficits nova unilateral secundária à depleção de DA, mas também mostram que a rotorod pode fornecer uma ferramenta robusta para a análise qualitativa do movimento.

Watch this Scientific Journal Video about Use of Rotorod as a Method for the Qualitative Analysis of Walking in Rat at JoVE.com

Use of Rotorod as a Method for the Qualitative Analysis of Walking in Rat

O teste rotorod é usado para avaliar o estado do motor no movimento de caminhar de ratos hemi-Parkinson analógica.

Uso de Rotorod como um método de análise qualitativa de Walking in Rat

High speed videoanalysis of the details of movement can provide a source of information about qualitative aspects of walking movements. When walking on a ...

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