Name: use of rotorod as a method for the qualitative analysis of walking in rat
Uploaded: 2008-12-10T00:00:00
Description: Watch this Scientific Journal Video about Use of Rotorod as a Method for the Qualitative Analysis of Walking in Rat at JoVE.com

Поддержке грантов от канадского института исследований в области здравоохранения, канадские Stroke сети и Альберта Heritage Foundation для медицинских исследований.

Rotorod ходьбе аппарата Rotorod был вращающийся цилиндр, 4 см в диаметре, фиксированные 35 см над землей и заключены в прозрачную оргстекла. Цилиндр rotorod была покрыта текстурированной резиновое покрытие, что облегчает сцепление с дорогой. Этаже аппарат был покрыт слоем пены для пр...

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Целью настоящего исследования было изучить вопрос о rotorod тест для грызунов навыков передвижения могут быть использованы для качественного рассмотрения осанку, лапы степпинг и задних конечностей вмешалась в нервной системе ранения крыс. Для анализа, крысы с односторонним истощения DA и контрольн?...

A correction was made to: <a href="http://www.jove.com/video/1030/">Use of Rotorod as a Method for the Qualitative Analysis of Walking in Rat</a>. An additional paragraph was added to the discussion, the abstract was modified, and another reference was included.
Additional reference:
15. Whishaw IQ, Li K, Whishaw PA, Gorny B, Metz GA. Distinct forelimb and hind limb stepping impairments in unilateral dopamine-depleted rats: use of the rotorod as a method for the qualitative analysis of skilled walking. J Neurosci Meth 126, 13-23 (2003).
Revised Abstract:
High speed videoanalysis of the details of movement can provide a source of information about qualitative aspects of walking movements. When walking on a rotorod, animals remain in approximately the same place making repetitive movements of stepping. Thus the task provides a rich source of information on the details of foot stepping movements. Subjects were hemi-Parkinson analogue rats, produced by injection of 6-hydroxydopamine (6-OHDA) into the right nigrostriatal bundle to deplete nigrostriatal dopamine (DA). The present report provides a video analysis illustration of animals previously were filmed from frontal, lateral, and posterior views as they walked (15). Rating scales and frame-by-frame replay of the video records of stepping behavior indicated that the hemi-Parkinson rats were chronically impaired in posture and limb use contralateral to the DA-depletion. The contralateral limbs participated less in initiating and sustaining propulsion than the ipsilateral limbs. These deficits secondary to unilateral DA-depletion show that the rotorod provides a use task for the analysis of stepping movements.
Additional Discussion Paragraph:
A more detailed presentation of the present study has been made (Whishaw et al, 2003), but the present study presents the video support describing the stepping movement in the good and affected limbs of unilateral dopamine-depleted rats. For the analysis, rats with unilateral DA depletions and control rats were video recorded from front, lateral and posterior views. A rating scale of posture and forelimb movements indicated that stepping movements were chronically impaired following surgery. Examination of limb movements indicated that whereas the DA-depleted rats could use the limbs contralateral to the lesion for support, they received minimal use for shifting weight. The results of this study indicate that the rotorod task, in addition to providing quantitative measures of motor impairments, can also provide insights into the qualitative impairments.
Original Abstract:
The rotorod test, in which animals walk on a rotating drum, is widely used to assess motor status in laboratory rodents. Performance is measured by the duration that an animal stays up on the drum as a function of drum speed. Here we report that the task provides a rich source of information about qualitative aspects of walking movements. Because movements are performed in a fixed location, they can readily be examined using high-speed video recording methods. The present study was undertaken to examine the potential of the rotorod to reveal qualitative changes in the walking movements of hemi-Parkinson analogue rats, produced by injection of 6-hydroxydopamine (6-OHDA) into the right nigrostriatal bundle to deplete nigrostriatal dopamine (DA). Beginning on the day following surgery and then periodically over the next two months, the rats were filmed from frontal, lateral, and posterior views as they walked on the rotorod. Behavior was analyzed by frame-by-frame replay of the video records. Rating scales of stepping behavior indicated that the hemi-Parkinson rats were chronically impaired in their posture and in the use of the limbs contralateral to the DA-depletion. The contralateral limbs not only displayed postural and movement abnormalities, they participated less in initiating and sustaining propulsion than did the ipsilateral limbs. These findings not only reveal new deficits secondary to unilateral DA-depletion, but also show that the rotorod can provide a robust tool for the qualitative analysis of movement.

A correction was made to: <a href="http://www.jove.com/video/1030/">Use of Rotorod as a Method for the Qualitative Analysis of Walking in Rat</a>. An additional paragraph was added to the discussion, the abstract was modified and another reference was included.

Erratum: Use of Rotorod as a Method for the Qualitative Analysis of Walking in Rat

use of rotorod as a method for the qualitative analysis of walking in rat

Rotorod испытания, в которых животные ходить на вращающемся барабане, широко используется для оценки состояния двигателя в лабораторных грызунов. Производительность измеряется длительность, что животное остается на барабан в зависимости от скорости барабана. Здесь мы сообщаем, что задача содержит богатый источник информации о качественных аспектов движения ходьбы. Потому что движения выполняются в определенном месте, они могут быть легко изучены с помощью высокоскоростных методов записи видео. Настоящее исследование было предпринято для изучения потенциала rotorod выявить качественные изменения в ходьбе движения геми-Паркинсона крыс аналога, производится путем введения 6-hydroxydopamine (6-OHDA) в правую нигростриатной расслоение к разрушающим нигростриатной допамина (ДА ). Начиная с операции следующий день, а затем периодически в течение ближайших двух месяцев, крысы были сняты с фронтальной, боковой и задней просмотра, как они шли по rotorod. Поведение была проанализирована кадр за кадром воспроизведения видеозаписей. Рейтинговые шкалы активизации поведения показали, что полу-Паркинсона крыс хроническими нарушениями в их осанке и в использовании конечностей контралатеральной для DA-истощение. Контралатеральной конечности не только отображаются постуральной и движения аномалии, они участвовали менее в инициировании и поддержании движения, чем ипсилатеральных конечностей. Эти данные не только выявить новые дефицит вторичных односторонних DA-истощение, но и показать, что rotorod может обеспечить надежный инструмент для качественного анализа движения.

Watch this Scientific Journal Video about Use of Rotorod as a Method for the Qualitative Analysis of Walking in Rat at JoVE.com

Use of Rotorod as a Method for the Qualitative Analysis of Walking in Rat

Rotorod тест используется для оценки состояния двигателя в ходьбе движение геми-Паркинсона крыс аналога.

Использование Rotorod как метод Качественный анализ Прогулка по Крыса

High speed videoanalysis of the details of movement can provide a source of information about qualitative aspects of walking movements. When walking on a ...

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