Name: use of rotorod as a method for the qualitative analysis of walking in rat
Uploaded: 2008-12-10T00:00:00
Description: Watch this Scientific Journal Video about Use of Rotorod as a Method for the Qualitative Analysis of Walking in Rat at JoVE.com

Apoyado por becas de los Institutos Canadienses de Investigación en Salud, la Red Canadiense de accidente cerebrovascular, y la Alberta Heritage Foundation para la Investigación Médica.

Rotorod aparato de caminar El rotorod era un cilindro giratorio, 4 cm de diámetro, fija 35 cm sobre el suelo y rodeado de plexiglás transparente. El cilindro rotorod estaba cubierta de revestimiento de goma de textura, lo que facilita la tracción. El suelo del aparato estaba cubierto con una capa de espuma para evitar lesiones cuando los animales se redujo. Un pequeño motor eléctrico siempre encendido para encender el rotorod a través de una correa de goma. El rotorod se estableció para ...

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El propósito del presente estudio fue examinar si la prueba de habilidad rotorod roedores locomoción se puede utilizar para el examen cualitativo de la postura, dando un paso patas delanteras y la extremidad posterior intensificación de las ratas del sistema nervioso lesionado. Para el análisis, las ratas con disminuciones unilaterales DA y el control de ratas fueron de vídeo grabados desde puntos de vista frontal, lateral y posterior. Una escala de valoración de la postura y los movimientos de extremidades anteriores indicaron que lo...

A correction was made to: <a href="http://www.jove.com/video/1030/">Use of Rotorod as a Method for the Qualitative Analysis of Walking in Rat</a>. An additional paragraph was added to the discussion, the abstract was modified, and another reference was included.
Additional reference:
15. Whishaw IQ, Li K, Whishaw PA, Gorny B, Metz GA. Distinct forelimb and hind limb stepping impairments in unilateral dopamine-depleted rats: use of the rotorod as a method for the qualitative analysis of skilled walking. J Neurosci Meth 126, 13-23 (2003).
Revised Abstract:
High speed videoanalysis of the details of movement can provide a source of information about qualitative aspects of walking movements. When walking on a rotorod, animals remain in approximately the same place making repetitive movements of stepping. Thus the task provides a rich source of information on the details of foot stepping movements. Subjects were hemi-Parkinson analogue rats, produced by injection of 6-hydroxydopamine (6-OHDA) into the right nigrostriatal bundle to deplete nigrostriatal dopamine (DA). The present report provides a video analysis illustration of animals previously were filmed from frontal, lateral, and posterior views as they walked (15). Rating scales and frame-by-frame replay of the video records of stepping behavior indicated that the hemi-Parkinson rats were chronically impaired in posture and limb use contralateral to the DA-depletion. The contralateral limbs participated less in initiating and sustaining propulsion than the ipsilateral limbs. These deficits secondary to unilateral DA-depletion show that the rotorod provides a use task for the analysis of stepping movements.
Additional Discussion Paragraph:
A more detailed presentation of the present study has been made (Whishaw et al, 2003), but the present study presents the video support describing the stepping movement in the good and affected limbs of unilateral dopamine-depleted rats. For the analysis, rats with unilateral DA depletions and control rats were video recorded from front, lateral and posterior views. A rating scale of posture and forelimb movements indicated that stepping movements were chronically impaired following surgery. Examination of limb movements indicated that whereas the DA-depleted rats could use the limbs contralateral to the lesion for support, they received minimal use for shifting weight. The results of this study indicate that the rotorod task, in addition to providing quantitative measures of motor impairments, can also provide insights into the qualitative impairments.
Original Abstract:
The rotorod test, in which animals walk on a rotating drum, is widely used to assess motor status in laboratory rodents. Performance is measured by the duration that an animal stays up on the drum as a function of drum speed. Here we report that the task provides a rich source of information about qualitative aspects of walking movements. Because movements are performed in a fixed location, they can readily be examined using high-speed video recording methods. The present study was undertaken to examine the potential of the rotorod to reveal qualitative changes in the walking movements of hemi-Parkinson analogue rats, produced by injection of 6-hydroxydopamine (6-OHDA) into the right nigrostriatal bundle to deplete nigrostriatal dopamine (DA). Beginning on the day following surgery and then periodically over the next two months, the rats were filmed from frontal, lateral, and posterior views as they walked on the rotorod. Behavior was analyzed by frame-by-frame replay of the video records. Rating scales of stepping behavior indicated that the hemi-Parkinson rats were chronically impaired in their posture and in the use of the limbs contralateral to the DA-depletion. The contralateral limbs not only displayed postural and movement abnormalities, they participated less in initiating and sustaining propulsion than did the ipsilateral limbs. These findings not only reveal new deficits secondary to unilateral DA-depletion, but also show that the rotorod can provide a robust tool for the qualitative analysis of movement.

A correction was made to: <a href="http://www.jove.com/video/1030/">Use of Rotorod as a Method for the Qualitative Analysis of Walking in Rat</a>. An additional paragraph was added to the discussion, the abstract was modified and another reference was included.

Erratum: Use of Rotorod as a Method for the Qualitative Analysis of Walking in Rat

use of rotorod as a method for the qualitative analysis of walking in rat

La prueba rotorod, en el que los animales caminen sobre un tambor giratorio, es ampliamente utilizado para evaluar el estado del motor en los roedores de laboratorio. El rendimiento se mide por el tiempo que un animal se mantiene hasta en el tambor en función de la velocidad del tambor. Aquí mostramos que la tarea es una fuente rica de información sobre los aspectos cualitativos de los movimientos de caminar. Debido a que los movimientos se realizan en una ubicación fija, que fácilmente se puede examinar utilizando métodos de alta velocidad de grabación de vídeo. El presente estudio se realizó para examinar el potencial de la rotorod para revelar los cambios cualitativos en los movimientos a pie de hemi-Parkinson ratas analógica, producida por la inyección de 6-hidroxidopamina (6-OHDA) en el paquete nigroestriatal derecho a agotar la dopamina nigroestriatal (DA ). A partir del día siguiente de la cirugía y luego periódicamente durante los próximos dos meses, las ratas fueron filmadas desde puntos de vista frontal, lateral y posterior, mientras caminaban en el rotorod. Comportamiento fue analizado por reproducir fotograma a fotograma de las grabaciones de vídeo. Las escalas de calificación de dar un paso comportamiento indica que las ratas hemi-Parkinson fueron dañados de forma crónica en su postura y en el uso de las extremidades contralateral a la DA-agotamiento. Las extremidades contralateral no sólo se muestra anomalías posturales y de movimiento, que participó menos en el inicio y el mantenimiento de propulsión que hizo las extremidades ipsilateral. Estos resultados no sólo revelan nuevos déficits unilateral secundaria a DA-agotamiento, pero también muestran que el rotorod puede proporcionar una sólida herramienta para el análisis cualitativo del movimiento.

Watch this Scientific Journal Video about Use of Rotorod as a Method for the Qualitative Analysis of Walking in Rat at JoVE.com

Use of Rotorod as a Method for the Qualitative Analysis of Walking in Rat

La prueba rotorod se utiliza para evaluar el estado del motor en el movimiento de caminar de la hemi-Parkinson ratas analógica.

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