Name: use of rotorod as a method for the qualitative analysis of walking in rat
Uploaded: 2008-12-10T00:00:00
Description: Watch this Scientific Journal Video about Use of Rotorod as a Method for the Qualitative Analysis of Walking in Rat at JoVE.com

Finansierats med bidrag från den kanadensiska Institutes of Health Research, den kanadensiska Stroke Network, och Alberta Heritage Foundation för medicinsk forskning.

Rotorod gå apparater Det rotorod var en roterande cylinder, 4 cm i diameter, fast 35 cm över marken och omges av transparent plexiglas. Den rotorod cylindern var täckt av texturerat gummibeläggning, vilket underlättar dragkraft. Golvet i apparaten var täckt med ett lager av skum för att förhindra skador när djur föll. En liten elmotor som makt att vända rotorod via ett gummiband. Den rotorod sattes att rotera en gång var 5: s. Videoinspelningar och kinemati...

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Syftet med denna studie var att undersöka om rotorod testet för gnagare förflyttning kompetens kan användas för kvalitativ undersökning av hållning, forelimb kliva och bakbenen kliva i nervös skadade systemet råttor. För analys, råttor med unilateral DA kundledet och råttor styra video som spelats in från framsidan, sido-och bakre åsikter. En skattningsskala för kroppshållning och rörelser forelimb visade att kliva rörelser kroniskt var nedsatt efter operation. Granskning av benrörelser uppgav att det i DA-utarmat råttor...

A correction was made to: <a href="http://www.jove.com/video/1030/">Use of Rotorod as a Method for the Qualitative Analysis of Walking in Rat</a>. An additional paragraph was added to the discussion, the abstract was modified, and another reference was included.
Additional reference:
15. Whishaw IQ, Li K, Whishaw PA, Gorny B, Metz GA. Distinct forelimb and hind limb stepping impairments in unilateral dopamine-depleted rats: use of the rotorod as a method for the qualitative analysis of skilled walking. J Neurosci Meth 126, 13-23 (2003).
Revised Abstract:
High speed videoanalysis of the details of movement can provide a source of information about qualitative aspects of walking movements. When walking on a rotorod, animals remain in approximately the same place making repetitive movements of stepping. Thus the task provides a rich source of information on the details of foot stepping movements. Subjects were hemi-Parkinson analogue rats, produced by injection of 6-hydroxydopamine (6-OHDA) into the right nigrostriatal bundle to deplete nigrostriatal dopamine (DA). The present report provides a video analysis illustration of animals previously were filmed from frontal, lateral, and posterior views as they walked (15). Rating scales and frame-by-frame replay of the video records of stepping behavior indicated that the hemi-Parkinson rats were chronically impaired in posture and limb use contralateral to the DA-depletion. The contralateral limbs participated less in initiating and sustaining propulsion than the ipsilateral limbs. These deficits secondary to unilateral DA-depletion show that the rotorod provides a use task for the analysis of stepping movements.
Additional Discussion Paragraph:
A more detailed presentation of the present study has been made (Whishaw et al, 2003), but the present study presents the video support describing the stepping movement in the good and affected limbs of unilateral dopamine-depleted rats. For the analysis, rats with unilateral DA depletions and control rats were video recorded from front, lateral and posterior views. A rating scale of posture and forelimb movements indicated that stepping movements were chronically impaired following surgery. Examination of limb movements indicated that whereas the DA-depleted rats could use the limbs contralateral to the lesion for support, they received minimal use for shifting weight. The results of this study indicate that the rotorod task, in addition to providing quantitative measures of motor impairments, can also provide insights into the qualitative impairments.
Original Abstract:
The rotorod test, in which animals walk on a rotating drum, is widely used to assess motor status in laboratory rodents. Performance is measured by the duration that an animal stays up on the drum as a function of drum speed. Here we report that the task provides a rich source of information about qualitative aspects of walking movements. Because movements are performed in a fixed location, they can readily be examined using high-speed video recording methods. The present study was undertaken to examine the potential of the rotorod to reveal qualitative changes in the walking movements of hemi-Parkinson analogue rats, produced by injection of 6-hydroxydopamine (6-OHDA) into the right nigrostriatal bundle to deplete nigrostriatal dopamine (DA). Beginning on the day following surgery and then periodically over the next two months, the rats were filmed from frontal, lateral, and posterior views as they walked on the rotorod. Behavior was analyzed by frame-by-frame replay of the video records. Rating scales of stepping behavior indicated that the hemi-Parkinson rats were chronically impaired in their posture and in the use of the limbs contralateral to the DA-depletion. The contralateral limbs not only displayed postural and movement abnormalities, they participated less in initiating and sustaining propulsion than did the ipsilateral limbs. These findings not only reveal new deficits secondary to unilateral DA-depletion, but also show that the rotorod can provide a robust tool for the qualitative analysis of movement.

A correction was made to: <a href="http://www.jove.com/video/1030/">Use of Rotorod as a Method for the Qualitative Analysis of Walking in Rat</a>. An additional paragraph was added to the discussion, the abstract was modified and another reference was included.

Erratum: Use of Rotorod as a Method for the Qualitative Analysis of Walking in Rat

use of rotorod as a method for the qualitative analysis of walking in rat

Den rotorod test, där djur gå på en roterande trumma, används ofta för att bedöma motoriska status i smågnagare. Prestanda mäts genom den tid som ett djur stannar upp på trumman som en funktion av trummans hastighet. Här rapporterar vi att uppgiften ger en rik källa till information om kvalitativa aspekter av promenader rörelser. Eftersom rörelserna utförs på en fast plats, kan de lätt undersökas med hjälp av snabba metoder för videoinspelning. Den aktuella studien genomfördes för att undersöka potentialen i rotorod att avslöja kvalitativa förändringar i går rörelser hemi-Parkinson analog råttor, producerad av injektion av 6-hydroxydopamine (6-OHDA) i rätt nigrostriatala bunt att tömma nigrostriatala dopamin (DA ). Börjar dagen efter operation och därefter med jämna mellanrum under de kommande två månaderna, var råttorna filmade från frontal, lateral och posterior åsikter som de gick på rotorod. Beteende analyserades för ruta för bildruta repris av videoinspelningar. Skattningsskalor att intensifiera beteende visade att hemi-Parkinson råttor kroniskt var nedsatt i sin hållning och i användningen av benen kontralateral till DA-utarmning. Den kontralaterala extremiteter inte bara visas postural och rörelse avvikelser deltog de mindre i att initiera och upprätthålla framdrivning än gjorde den ipsilaterala extremiteterna. Dessa fynd inte bara visa på nya underskott sekundärt till unilateral DA-utarmning, men också visa att rotorod kan ge ett kraftfullt verktyg för kvalitativ analys av rörelse.

Watch this Scientific Journal Video about Use of Rotorod as a Method for the Qualitative Analysis of Walking in Rat at JoVE.com

Use of Rotorod as a Method for the Qualitative Analysis of Walking in Rat

Den rotorod test används för att bedöma motoriska status i gång flödet av hemi-Parkinson analog råttor.

Användning av Rotorod som en metod för kvalitativ analys av Walking i Rat

High speed videoanalysis of the details of movement can provide a source of information about qualitative aspects of walking movements. When walking on a ...

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