Name: single molecule methods for monitoring changes in bilayer elastic properties
Uploaded: 2008-11-03T00:00:00
Duration: 12 min 20 s
Description: Watch this Scientific Journal Video about Single Molecule Methods for Monitoring Changes in Bilayer Elastic Properties at JoVE.com

请访问<a href="http://springerprotocols.com/index.vm">斯普林格协议</a> ，准备人工双层和使用探测细胞膜弹性的短杆菌肽渠道的详细信息。

The authors have nothing to disclose.

single molecule methods for monitoring changes in bilayer elastic properties

膜蛋白的功能是调节细胞膜的脂质成分。本规例是由于特定的脂质 - 蛋白质相互作用和更一般的脂质双分子层，蛋白质相互作用的结合。这些交互是特别重要的药理研究，作为当前药品市场上的许多可以改变脂质双层材料的属性，从而导致改变膜蛋白的功能。短杆菌肽通道的形成依赖而后者又依赖于脂质属性的短杆菌肽亚基的构象变化。因此，短杆菌肽通道电流是由于某些化合物的双层改变属性的记者。

Watch this Scientific Journal Video about Single Molecule Methods for Monitoring Changes in Bilayer Elastic Properties at JoVE.com

Single Molecule Methods for Monitoring Changes in Bilayer Elastic Properties

膜蛋白的功能是调节细胞膜的脂质成分。此视频文章详细介绍了如何改变膜性能的记者使用的双层贴片电极，以及如何使用短杆菌肽渠道，形成了一个补丁。

双层弹性性能的监测变化的单分子方法

Membrane protein function is regulated by the cell membrane lipid composition.  This regulation is due to a combination of specific lipid-protein ...

Research

JoVE Journal

Biology

Visualizing Single-molecule DNA Replication with Fluorescence Microscopy

可视化的单分子荧光显微镜下的DNA复制

We describe a simple fluorescence microscopy-based real-time method for observing DNA replication at the single-molecule level. A circular, forked DNA ...

科研

生物学

Automated System for Single Molecule Fluorescence Measurements of Surface-immobilized Biomolecules

表面固定生物分子的单分子荧光检测的自动化系统

Fluorescence Resonance Energy Transfer (FRET) microscopy has been widely used to study the structure and dynamics of molecules of biological interest, ...

Single-Molecule Imaging of Nuclear Transport

单分子成像核运输

The utility of single molecule fluorescence microscopy approaches has been proven to be of a great avail in understanding biological reactions over the ...

Gramicidin-based Fluorescence Assay; for Determining Small Molecules Potential for Modifying Lipid Bilayer Properties

短杆菌肽为基础的荧光分析法，确定小分子的修改脂质双层属性的潜在

Many drugs and other small molecules used to modulate biological function are amphiphiles that adsorb at the bilayer/solution interface and thereby alter ...

Monitoring Equilibrium Changes in RNA Structure by 'Peroxidative' and 'Oxidative' Hydroxyl Radical Footprinting

监测RNA结构平衡的变动情况“过氧化”和“氧化”羟基自由基足

RNA molecules play an essential role in biology. In addition to transmitting genetic information, RNA can fold into unique tertiary structures fulfilling ...

Long-term Lethal Toxicity Test with the Crustacean Artemia franciscana

Long-term Lethal Toxicity Test with the Crustacean Artemia franciscana

致命的甲壳类动物长期毒性试验卤虫franciscana</em

Our research activities target the use of biological methods for the evaluation of environmental quality, with particular reference to saltwater/brackish ...

AFM-based Mapping of the Elastic Properties of Cell Walls: at Tissue, Cellular, and Subcellular Resolutions

细胞壁的弹性性能的原子力显微镜为基础的映射：在组织，细胞，亚细胞和决议

We describe a recently developed method to measure mechanical properties of the surfaces of plant tissues using atomic force microscopy (AFM) ...

Studying DNA Looping by Single-Molecule FRET

研究DNA环接了单分子荧光共振能量转移

Bending of double-stranded DNA (dsDNA) is associated with many important biological processes such as DNA-protein recognition and DNA packaging into ...

A Proteoliposome-Based Efflux Assay to Determine Single-molecule Properties of Cl- Channels and Transporters

A Proteoliposome-Based Efflux Assay to Determine Single-molecule Properties of Cl- Channels and Transporters

一个脂蛋白体为基础的外排检测，以确定氯的单分子性质<sup&gt; -</sup&gt;通道和转运

The last 15 years have been characterized by an explosion in the ability to overexpress and purify membrane proteins from prokaryotic organisms as well as ...

Xenopus Oocytes: Optimized Methods for Microinjection, Removal of Follicular Cell Layers, and Fast Solution Changes in Electrophysiological Experiments

Xenopus Oocytes: Optimized Methods for Microinjection, Removal of Follicular Cell Layers, and Fast Solution Changes in Electrophysiological Experiments

爪蟾卵母：显微注射方法优化，滤泡细胞层的去除，并在电生理实验快速变化的解决方案

The Xenopus oocyte as a heterologous expression system for proteins, was first described by Gurdon et al.1 and has been widely used since its discovery ...