Single Molecule Methods for Monitoring Changes in Bilayer Elastic Properties

DOI :

10.3791/1032-v

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12:20 min

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November 3rd, 2008

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9,035 Views

Helgi Ingolfson¹, Ruchi Kapoor², Shemille A. Collingwood², Olaf Sparre Andersen²

¹Department of Physiology and Biophysics, Weill Cornell Medical College, ²Department of Physiology and Biophysics, Weill Cornell Medical College of Cornell University

Chapters

Membrane protein function is regulated by the cell membrane lipid composition. This video-article details how to form a patch using bilayer patch electrodes, as well as how to use gramicidin channels as reporters of altered membrane properties.