Name: human in-vivo bioassay for the tissue-specific measurement of nociceptive and inflammatory mediators
Uploaded: 2008-12-01T00:00:00
Description: Watch this Scientific Journal Video about Human In-Vivo Bioassay for the Tissue-Specific Measurement of Nociceptive and Inflammatory Mediators at JoVE.com

<p class="jove_title">诱导实验性炎症： </p><ol><li>第一度晒伤引起的非皮肤晒黑了一个校准，紫外线B的源（Saalmann万用表SBB的LT 499，Saalmann有限责任公司，禧福，德国）的援助大腿。晒伤是一个微透析实验开始前三次运用必要的紫外线光量，导致完成泛红裸露的皮肤，或3倍最小红斑剂量（MED）诱导24小时。 MED是在研究开始之前，每个主题确定。准确地传递是有保证的能源与紫外线米Grobel紫外线博士电子公司，埃特林根?...

<ol>
	<li>Angst, M. S., Clark, J. D., Carvalho, B., Tingle, M., Schmelz, M., Yeomans, D. C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Cytokine+profile+in+human+skin+in+response+to+experimental+inflammation,+noxious+stimulation+and+administration+of+a+COX-inhibitor:+A+microdialysis+study.">Cytokine profile in human skin in response to experimental inflammation, noxious stimulation and administration of a COX-inhibitor: A microdialysis study.</a> <em style='font-style: italic'>Pain</em>. <b>139</b>, 15-27 (2008).</li></ol>

<p>微透析技术和多重免疫检测技术的结合使用，是一个宝贵的实时，人体内生物活性提供利益的组织的生化事件的洞察力。结合行为测试，如疼痛测试，该方法允许调解疼痛和炎症的生化物质之间复杂的相互作用研究。有前途的方法是1）确定组织特定的生物标志物介导的疼痛和炎症，2）提供到与周围组织的病变炎症和慢性疼痛的条件的病理机理的认识，以及3）探索镇痛/反hyperalgesic和抗炎行动新的候选药物和介入...

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<td>Microdialysis catheter</td>
<td>Tool</td>
<td>Dermal Dialysis</td>
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<td>Dr. Grobel UV Elektronik GmbH</td>
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<td>Complete Proteinase Inhibitor</td>
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<td>Astra Zeneca</td>
<td>NA</td>
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<td>Becton Dickinson Co</td>
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human in-vivo bioassay for the tissue-specific measurement of nociceptive and inflammatory mediators

<p class="jove_content">这在人体内生物活性，可用于研究人类的志愿者和患者身上。样品收集相关组织的网站，如通过无菌插入微透析导管（皮肤透析，德国埃尔兰根）皮肤。这个例子说明的是收集实验发炎的皮肤在人类志愿者的组织间液。可以结合其他实验测试样品的采集。例如，当地公布的生物化学和主观实验发炎的皮肤疼痛刺激的敏感性的同时评估提供了关键的生化行为的环节，以确定疼痛和炎症的生物标志物。机械洞察生活的人的机体检测允许特定组织底层的疼痛和/或炎症过程。该方法也适合于研究现有的或新的干预措施的有效性 - 目标的痛苦和/或炎症性疾病的治疗 - 如新的候选药物。本文将收集人类研究对象的实验发炎的患处皮肤组织间液微透析技术的使用提供了详细的说明。通常处理多重珠阵列，使体积小50微升样品化验100分析物免疫援助组织间液样本。</p>

Watch this Scientific Journal Video about Human In-Vivo Bioassay for the Tissue-Specific Measurement of Nociceptive and Inflammatory Mediators at JoVE.com

Human In-Vivo Bioassay for the Tissue-Specific Measurement of Nociceptive and Inflammatory Mediators

<p class="jove_content">一个方法是为调解疼痛和炎症的生化物质的测量（在这里，实验发炎的皮肤）的相关组织网站的间质液样品在体内收集。</p>

组织特异性的伤害性和炎症介质的测量人体内生物活性测定

This in-vivo human bioassay can be used to study human volunteers and patients. Samples are collected from pertinent tissue sites such as the skin via ...

Research

JoVE Journal

Biology

In vivo Micro-circulation Measurement in Skeletal Muscle by Intra-vital Microscopy

在体内微循环在骨骼肌内重要的显微镜测量

BACKGROUND: Regulatory factors and detailed physiology of in vivo microcirculation have remained not fully clarified after many different modalities of ...

科研

生物学

Collecting and Measuring Nociceptive and Inflammatory Mediators in Surgical Wounds 

在手术伤口的痛觉及炎症介质的收集和测量

We describe a methodology by which we are able to collect and measure inflammatory and nociceptive biochemical mediators at the surgical wound site. ...

Measurement of Vacuolar and Cytosolic pH <em>In Vivo</em> in Yeast Cell Suspensions

Measurement of Vacuolar and Cytosolic pH In Vivo in Yeast Cell Suspensions

液泡和胞质pH值的测量<em&gt;在体内</em在酵母细胞悬浮液

Vacuolar and  cytosolic pH are highly regulated in yeast cells and occupy a central role in  overall pH homeostasis. We describe  protocols for ...

<em>In Vivo</em> Modeling of the Morbid Human Genome using <em>Danio rerio</em>

In Vivo Modeling of the Morbid Human Genome using Danio rerio

<em>在体内</em>病态的人类基因组建模使用<em>斑马鱼</em>

Here,  we present methods for the development of assays to query potentially clinically  significant nonsynonymous changes using in  vivo complementation ...

A Simple Bioassay for the Evaluation of Vascular Endothelial Growth Factors

一个简单的生物测定血管内皮生长因子的评价

The analysis of receptor tyrosine kinases and their interacting ligands involved in vascular biology is often challenging due to the constitutive ...

Gap Junctional Intercellular Communication: A Functional Biomarker to Assess Adverse Effects of Toxicants and Toxins, and Health Benefits of Natural Products

间隙连接通讯：天然产物的生物标志物的功能，以评估毒物和毒素的不利影响，以及健康的益处

This protocol describes a scalpel loading-fluorescent dye transfer (SL-DT) technique that measures intercellular communication through gap junction ...

Murine Aortic Crush Injury: An Efficient <em>In Vivo</em> Model of Smooth Muscle Cell Proliferation and Endothelial Function

Murine Aortic Crush Injury: An Efficient In Vivo Model of Smooth Muscle Cell Proliferation and Endothelial Function

小鼠主动脉夹层损伤：高效<em&gt;体内</em&gt;平滑肌细胞增殖和内皮功能模型

Arterial reconstruction, whether angioplasty or bypass surgery, involves iatrogenic trauma causing endothelial disruption and vascular smooth muscle cell ...

Detecting and Characterizing Protein Self-Assembly In Vivo by Flow Cytometry

通过流动细胞测定检测和描述 Vivo 中的蛋白质自组装

Protein self-assembly governs protein function and compartmentalizes cellular processes in space and time. Current methods to study it suffer from ...

Using <em>Caenorhabditis elegans</em> to Screen for Tissue-Specific Chaperone Interactions

Using Caenorhabditis elegans to Screen for Tissue-Specific Chaperone Interactions

使用 <em>卡诺哈布迪炎埃莱甘斯</em> 筛选组织特异性查佩龙相互作用

Correct folding and assembly of proteins and protein complexes are essential for cellular function. Cells employ quality control pathways that correct, ...

In vivo Measurement of Knee Extensor Muscle Function in Mice

小鼠膝盖外展肌肉功能的体内测量

Skeletal muscle plasticity in response to countless conditions and stimuli mediates concurrent functional adaptation, both negative and positive. In the ...