Name: micro-drive array for chronic <em>in vivo</em> recording: tetrode assembly
Uploaded: 2009-04-22T00:00:00
Description: Watch this Scientific Journal Video about Micro-drive Array for Chronic in vivo Recording: Tetrode Assembly at JoVE.com

<p class="jove_content">我们想确认为自动，电动四极管绞机设备在视频描述他的设计和建设，军山本。此外，我们要感谢所有过去和现在的威尔逊实验室成员的贡献微驱动器和四极管在实验室的技术开发。 </p>

<p class="jove_title"> 1。制备四极管</p><ol><li>首先获得一个50厘米长的一个四极建设，聚酰亚胺绝缘，镍铬丝与一个直径为12.5微米的核心。折了一半的线。沿对你的手指，使它们粘在一起。确保对电线在其整个长度有良好的接触。 </li><li>对折，再由两端。确保一端形成的循环是不扭结。切使四个秘诀是一致的四个线对非循环结束。 </li><li>对于下一步，您将需要修改的鳄鱼夹，电动翻转装置，单杠和一个以上的转动设?...

<ol>
	<li>Gray, C. M., Maldonado, P. E., Wilson, M., McNaughton, B. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Tetrodes+markedly+improve+the+reliability+and+yield+of+multiple+single-unit+isolation+from+multi-unit+recordings+in+cat+striate+cortex.">Tetrodes markedly improve the reliability and yield of multiple single-unit isolation from multi-unit recordings in cat striate cortex.</a> <em style='font-style: italic'>J. Neurosci. Methods</em>. <b>63</b> (1-2), 43-54 (1995).</li></ol>

<p class="jove_content">该协议是两部分协议的第二批：“微阵列的驱动器，用于慢性在体内录制。”相结合的微驱动器阵列和四极是获得一个清醒的许多神经元的同步录音功能强大的工具，表现动物准备。希望，我们已经提供了所有必要的信息，你会需要开始在自己的实验室或改进的电生理记录。 </p><p class="jove_content">机动四极捻线设备定制的设计和制造。我们提供了一个供应商和部分的数量，将允许您要订购的具有?...

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		<div>
		<table border="1">
		<thead>
		<tr>
		<th>Material Name</th>
		<th>Type</th>
		<th>Company</th>
		<th>Catalogue Number</th>
		<th>Comment</th>
		</tr>
		</thead>
		<tbody>
		
<tr>
<td>Panavise</td>
<td>Tool</td>
<td>Panavise</td>
<td>Model 301</td>
<td>Holds either micro-drive array or serrated scissors</td>
</tr>
<tr>
<td>Modified Xcelite 378M Pliers</td>
<td>Tool</td>
<td>Newark</td>
<td>96F8903</td>
<td>For inserting connector board pins.  To make, remove 2mm from one tip of the pliers.</td>
</tr>
<tr>
<td>Slow Cure Glue</td>
<td>Material</td>
<td>Great Planes (Champaign, IL)</td>
<td>GPMR6015</td>
<td>To glue the tetrode to the micro-drive</td>
</tr>
<tr>
<td>Gold Plating Solution</td>
<td>Material</td>
<td>Neuralynx</td>
<td>Sifco Selectron Gold NC</td>
<td>Contains gold particles for electroplating</td>
</tr>
<tr>
<td>Current Generator</td>
<td>Tool</td>
<td>World Precision Instruments</td>
<td>A365D-B</td>
<td>A.K.A. Stimulus Isolator.  Used to electroplate gold solution onto electrode tips.</td>
</tr>
<tr>
<td>Impedance Meter</td>
<td>Tool</td>
<td>BAK Electronics</td>
<td>IMP-1 / 6662 / 2788</td>
<td>To check impedance of electrodes</td>
</tr>
<tr>
<td>Hot Air Gun</td>
<td>Tool</td>
<td>Steinel (Germany)</td>
<td>HG 3002 LCD</td>
<td>To fuse electrodes together to make tetrode</td>
</tr>
<tr>
<td>Accu-Tek Carbofib Tip Tweezers</td>
<td>Tool</td>
<td>Aven (Ann Arbor, MI)</td>
<td>18768 (Pattern 304)</td>
<td>For handling tetrodes</td>
</tr>
<tr>
<td>Alligator Clip</td>
<td>Tool</td>
<td>Fine Science Tools</td>
<td>18050-35</td>
<td>A.K.A. Bulldog serrefine.  To clip the tetrodes together during twisting</td>
</tr>
<tr>
<td>Micro Dissecting Scissors</td>
<td>Tool</td>
<td>Biomedical Research Instruments</td>
<td>25-1000</td>
<td>For general cutting of tetrode/electrode wire</td>
</tr>
<tr>
<td>Iris Toughcut Serrated Scissors</td>
<td>Tool</td>
<td>Fine Science Tools</td>
<td>14058-09</td>
<td>For final cut of tetrodes</td>
</tr>
<tr>
<td>Teflon Coated Stainless Steel Wire</td>
<td>Material</td>
<td>A-M Systems (Carlsborg, WA)</td>
<td>790500</td>
<td>Ground wire</td>
</tr>
<tr>
<td>12.5 micron nichrome wire</td>
<td>Material</td>
<td>Kanthal Palm Coast</td>
<td>Rediohm-800 1/4 HARD PAC COATING</td>
<td>Polyimide coated micro-wire for making tetrodes.</td>
</tr>
<tr>
<td>Tetrode Twister</td>
<td>Tool</td>
<td>Neuralynx</td>
<td>Tetrode Assembly Station</td>
<td>Increases the speed and consistency of tetrode fabrication.

</td>
</tr>
<tr>
<td>Silver Paint</td>
<td>Material</td>
<td>GC Electronics</td>
<td>22-023</td>
<td>For connecting the micro-electrode wires of the tetrode to the connector pins (see Discussion)</td>
</tr>
<tr>
<td>X-Acto Knife Handle</td>
<td>Material</td>
<td>MSC Direct</td>
<td>X3001</td>
<td>For constructing a micro-drive holding device.</td>
</tr>
<tr>
<td>26 Pin, 2 Row Connector</td>
<td>Material</td>
<td>Mill-max</td>
<td>852-10-026-30-001000</td>
<td>For constructing a micro-drive holding device.</td>
</tr>		</tbody>
		</table>
		</div>
	

micro-drive array for chronic <em>in vivo</em> recording: tetrode assembly

<p class="jove_content">四极，捆绑四个电极，已被证明是一个有价值的工具，同时记录多个神经元在体内。差分幅度超过四极通道动作电位签名允许单机活动多单元信号的隔离。能够精确地控制脑立体定位的位置和四极管的深度，研究整个大脑区域的协调的神经活动的关键。在与一个微驱动器阵列相结合，这是可以实现精确定位和稳定的控制在几天或几周的过程中，许多四极管。在这个协议中，我们演示了如何制造和使用的基本工具和材料条件四极管，安装到一个长期的多驱动器的四极管阵列的四极管的录音，在大鼠，接地线连接的微型驱动器阵列，并在体内到的微驱动器阵列一个附加的保护为了保护环境与身体接触的四极管锥。</p>

Watch this Scientific Journal Video about Micro-drive Array for Chronic in vivo Recording: Tetrode Assembly at JoVE.com

Micro-drive Array for Chronic in vivo Recording: Tetrode Assembly

<p class="jove_content">在这个协议中，我们将演示如何制造和使用同一个微驱动器阵列，这是在大鼠慢性电生理记录设计条件四极管。此外，我们说明了微驱动器阵列的建设，其中包括安装接地线和保护锥的最后阶段。</p>

慢性的微型驱动器阵列<em>在体内</em>录音：四极大会

The tetrode, a bundle of four electrodes, has proven to be a valuable tool for the simultaneous recording of multiple neurons in-vivo.  The differential ...

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Micro-drive Array for Chronic <em>in vivo</em> Recording: Tetrode Assembly

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慢性的微型驱动器阵列在体内录音：四极大会