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DOI :
10.3791/1113-v
May 7th, 2009
Chapters
0:00
Title
0:54
Introduction
1:29
Preparation of RNA
3:26
Preparation of Morpholino
5:07
Preparing the Micropipette and Plates for Microinjections
7:51
Preparing and Injecting Fertilized Zebrafish Embryos
10:09
Representative Results: EGFP Expression and Mutant Phenotypes
11:07
Conclusion
显微注射是外来物质引入斑马鱼胚胎受精的完善而有效的方法。在这里,我们展示了一个强大的执行mRNA表达显微注射技术,和吗啉代寡核苷酸在斑马鱼的基因敲除研究。
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