Name: neurocircuit assays for seizures in epilepsy mutants of drosophila
Uploaded: 2009-04-15T00:00:00
Description: Watch this Scientific Journal Video about Neurocircuit Assays for Seizures in Epilepsy Mutants of Drosophila at JoVE.com

这项工作是支持由美国国立卫生研究院的研究经费，癫痫基金会赠款和McKnight基金会赠款吨果蝇果蝇的实验是由加州大学伯克利分校的动物使用委员会规定的准则和法规的规定执行。

样品制备： 实验前的夜晚，所需的基因型的雄蝇麻醉，置于一小瓶用柔软的尼龙插件。棉花球也将工作。麻醉后，癫痫发作阈值的改变，所以苍蝇是最好的左至少6个小时，虽然在夜间是理想的。 在实验的一天，苍蝇是固定在玻片一方的牙科用蜡层。 拾起头的使用了23号针头（锐利尖端切断）真空线连接到一个单一的飞。 <p class="jov...

<ol>
	<li>Kuebler, D., Tanouye, M. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Modifications+of+seizure+susceptibility+in+Drosophila.">Modifications of seizure susceptibility in Drosophila.</a> J Neurophysiol. 83, 998-1009 (2000).</li><li>Kuebler, D., Zhang, H., Ren, X., Tanouye, M. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Genetic+suppression+of+seizure+susceptibility+in+Drosophila.">Genetic suppression of seizure susceptibility in Drosophila.</a> J Neurophysiol. 86, 1211-1225 (2001).</li><li>Song, J., Tanouye, M. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Seizure+suppression+by+shakB2,+a+gap+junction+mutation+in+Drosophila.">Seizure suppression by shakB2, a gap junction mutation in Drosophila.</a> J Neurophysiol. 95, 627-635 (2006).</li></ol>

该视频已证明在确定个人果蝇飞的癫痫发作阈值的基本步骤。使用数字示波器，而是一个计算机化的数字化仪也可使用。这种技术是为确定药物或基因的改变上检获的一只苍蝇阈值的影响非常有用。

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neurocircuit assays for seizures in epilepsy mutants of drosophila

果蝇是研究扣押喜欢活动的一个有用的工具。一个可供扣押的活动和行为的各个方面的研究，其中癫痫发作可以通过或者人身触电或电刺激诱发的各种突变体。所有的苍蝇，包括野生型，将发生癫痫喜欢的活动，如果在足够高的电压的刺激。扣押喜欢的活动是一个全或无响应和每个基因型都有一个特定的癫痫发作阈值。特定基因型的飞癫痫发作阈值可以改变，可以通过药物治疗或基因抑制或增强。阈值是很容易通过电生理测量。海关检获走私活动直接传递到大脑，通过背纵肌（DLMS）在胸部记录通过高频电刺激可诱发。 DLMS支配巨头的光纤系统的一部分。低电压，高频率的刺激开始，随后提高电压较小的增量，为一个单一的飞癫痫发作阈值可以衡量的。

Watch this Scientific Journal Video about Neurocircuit Assays for Seizures in Epilepsy Mutants of Drosophila at JoVE.com

Neurocircuit Assays for Seizures in Epilepsy Mutants of Drosophila

使用高频电刺激发作，活动可诱导<em&gt;果蝇</em&gt;。这个活动是很容易从巨大的光纤系统记录。

Neurocircuit检测癫痫发作癫痫突变果蝇</em

Drosophila melanogaster is a useful tool for studying seizure like activity. A variety of mutants in which seizures can be induced through either physical ...

Research

JoVE Journal

Biology

Neurocircuit Assays for Seizures in Epilepsy Mutants of Drosophila

Paradigms for Pharmacological Characterization of C. elegans Synaptic Transmission Mutants

为线虫突触传递的突变体的药理特性的范式

The nematode, Caenorhabditis elegans, has become an expedient model for studying neurotransmission. C. elegans is unique among animal models, as the ...

科研

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One of the most pressing problems facing modern medical researchers is the surging levels of obesity, with the consequent increase in associated disorders ...

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The Drosophila embryo has long been a powerful laboratory model for elucidating molecular and genetic mechanisms that control development. The ease of ...