Name: monitoring plant hormones during stress responses
Uploaded: 2009-06-15T00:00:00
Description: Watch this Scientific Journal Video about Monitoring Plant Hormones During Stress Responses at JoVE.com

<ol><li>首先，我们需要准备提取2毫升螺丝帽小瓶：加入400ul提取缓冲液（1 - 丙醇：水：浓盐酸2:1:0.002卷/体积/体积）和10μL各自的内部标准（10ng/μl ）一小瓶。在液氮中冷冻，然后添加的陶瓷珠。 </li><li>添加植物材料（50至200毫克），而小瓶仍然定位在液氮。需要注意的是要估计的进一步处理之前，以便精确量化，这是基于对鲜重后，添加植物材料的重量。 </li><li>确保所有的液氮蒸发前紧紧地关闭第小瓶。请注意?...

<ol>
	<li>Schmelz, E. A., Engelberth, J., Tumlinson, J. H., Alborn, H. T. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+use+of+vapor+phase+extraction+in+metabolic+profiling+of+phytohormones+and+other+metabolites.">The use of vapor phase extraction in metabolic profiling of phytohormones and other metabolites.</a> Plant Journal. 39, 790-808 (2004).</li><li>Engelberth, J., Alborn, H. T., Schmelz, E. A., Tumlinson, J. H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Airborne+signals+prime+plants+against+insect+herbivore+attack.">Airborne signals prime plants against insect herbivore attack.</a> PNAS. 101, 1781-1785 (2004).</li><li>Schmelz, E. A., Engelberth, J., Alborn, H. T., O&amp;acute, D. o. n. n. e. l., Sammons, P., Toshima, M., H, J. H. T. u. m. l. i. n. s. o. n. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Simultaneous+analysis+of+phytohormones,+phytotoxins,+and+volatile+organic+compounds+in+plants.">Simultaneous analysis of phytohormones, phytotoxins, and volatile organic compounds in plants.</a> PNAS. 100, 10552-10557 (2003).</li><li>Engelberth, J., Schmelz, E. A., Alborn, H., Cardoza, Y. J., Huang, J., Tumlinson, J. H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Simultaneous+Quantification+of+Jasmonic+acid+and+Salicylic+acid+by+Vapor+Phase+Extraction+and+Gas+Chromatography+-+Chemical+Ionization-Mass+Spectrometry.">Simultaneous Quantification of Jasmonic acid and Salicylic acid by Vapor Phase Extraction and Gas Chromatography - Chemical Ionization-Mass Spectrometry.</a> Analytical Biochemistry. 312, 242-250 (2003).</li></ol>

这里介绍的方法已被证明了植物信号的大小和被提取，甲基化，而蒸发的化合物的化学性质并不妨碍它的化合物广泛可靠的工作。此外，甲基化过程，因为它是本文所述，是不是衍生化的唯一途径。 Silyllation和乙酰化的替代方法和协议，可以很容易地在网上找到。 如果不能够监测记录在运行过程中应仅限于从过程中排除太多不必要的离子质量范围内的单离子质谱仪。例如，?...

monitoring plant hormones during stress responses

在植物响应各种环境刺激和压力调节植物激素和相关的信令化合物发挥了重要作用。其中最严重的应力是昆虫的取食，病原体的感染，干旱胁迫。对于其中的每一个讲一套特定的激素和/或组合被称为微调的反应，从而保证植物的生存。在这些反应的调控涉及的主要激素是茉莉酸（JA），水杨酸（SA）和脱落酸（ABA）。为了更好地理解个人激素的作用以及他们潜在的相互作用在这些反应，这是必要的，在一个时间以及空间的方式来监测其丰度的变化。对于这些和其他信号的化合物容易，灵敏度高，重现性好量化，我们开发了基于汽相萃取和气相色谱/质谱（GC / MS）分析（1，2，3，4）的方法。这些化合物从植物组织中提取的酸性水溶液后，1 - 丙醇与二氯甲烷混合羧酸化合物的甲基化，高温下挥发，在高分子吸水收集。后成样品瓶洗脱分析物是通过气相色谱分离和化学电离质谱检测。然后使用适当的内部标准，允许有关分析物和内标峰面积的简单量化。

Watch this Scientific Journal Video about Monitoring Plant Hormones During Stress Responses at JoVE.com

Monitoring Plant Hormones During Stress Responses

提供一个简单的方法是，允许小组织样本的多种植物激素的快速提取和分析。该程序使用气相萃取庄严的纯化步骤。 GC / MS化学电离主要生产（M +1）+离子的样品进行了分析。

植物激素在应激反应监测

Plant hormones and related signaling compounds play an important role in the regulation of plant responses to various environmental stimuli and stresses. ...

Research

JoVE Journal

Biology

Investigating Tissue- and Organ-specific Phytochrome Responses using FACS-assisted Cell-type Specific Expression Profiling in Arabidopsis thaliana

Investigating Tissue- and Organ-specific Phytochrome Responses using FACS-assisted Cell-type Specific Expression Profiling in Arabidopsis thaliana

调查组织和器官特定的光敏色素反应，使用流式细胞仪辅助细胞类型特定的表达谱拟南芥</em

Light mediates an array of developmental and adaptive processes throughout the life cycle of a plant. Plants utilize light-absorbing molecules called ...

科研

生物学

Bioassays for Monitoring Insecticide Resistance

生物测定对杀虫剂的抗药性监测

Pest resistance to pesticides is an increasing problem because pesticides are an integral part of high-yielding production agriculture. When few products ...

Monitoring Dynamic Changes In Mitochondrial Calcium Levels During Apoptosis Using A Genetically Encoded Calcium Sensor

使用一种基因编码的钙传感器来监测细胞凋亡过程中线粒体钙水平的动态变化

Dynamic changes in intracellular calcium concentration in response to various stimuli regulates many cellular processes such as proliferation, ...

Long-term Lethal Toxicity Test with the Crustacean Artemia franciscana

Long-term Lethal Toxicity Test with the Crustacean Artemia franciscana

致命的甲壳类动物长期毒性试验卤虫franciscana</em

Our research activities target the use of biological methods for the evaluation of environmental quality, with particular reference to saltwater/brackish ...

Linking Predation Risk, Herbivore Physiological Stress and Microbial Decomposition of Plant Litter

链接捕食风险，草食动物生理应激和植物凋落物的微生物分解

The quantity and quality of detritus entering the soil  determines the rate of decomposition by microbial communities as well as  recycle rates of ...

Analysis of Translation Initiation During Stress Conditions by Polysome Profiling

翻译起始的分析在应力条件由多聚核糖体剖析

Precise control of mRNA translation is fundamental for eukaryotic cell homeostasis, particularly in response to physiological and pathological stress. ...

Measuring Spatial and Temporal Ca2+ Signals in Arabidopsis Plants

Measuring Spatial and Temporal Ca2+ Signals in Arabidopsis Plants

测量时空的Ca<sup&gt; 2 +</sup&gt;在拟南芥信号

Developmental and environmental cues induce Ca2+ fluctuations in plant cells. Stimulus-specific spatial-temporal Ca2+ patterns are sensed by cellular Ca2+ ...

High-throughput CRISPR Vector Construction and Characterization of DNA Modifications by Generation of Tomato Hairy Roots

高通量CRISPR载体的构建及DNA修饰的表征番茄毛状根的一代

Targeted DNA mutations generated by vectors with clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 technology have proven useful for ...

Hydroponics: A Versatile System to Study Nutrient Allocation and Plant Responses to Nutrient Availability and Exposure to Toxic Elements

水培：一个多功能的系统来研究营养分配和植物响应养分有效性及暴露于有毒元素

Hydroponic systems have been utilized as one of the standard methods for plant biology research and are also used in commercial production for several ...

Split Green Fluorescent Protein System to Visualize Effectors Delivered from Bacteria During Infection

分离绿色荧光蛋白系统可视化细菌在感染过程中传递的效应

Bacteria, one of the most important causative agents of various plant diseases, secrete a set of effector proteins into the host plant cell to subvert the ...