Name: implantation of engineered tissue in the rat heart
Uploaded: 2009-06-24T00:00:00
Description: Watch this Scientific Journal Video about Implantation of Engineered Tissue in the Rat Heart at JoVE.com

<p class="jove_content">这项工作是从美国国立卫生研究院的研究补助金（HL068915 HL088206）和在波士顿儿童医院的心脏传导基金捐款支持。 </p>

<p class="jove_title">第1部分：全身麻醉诱导</p><ol><li>一个200-250克Lewis大鼠50毫克/公斤氯胺酮和4毫克/公斤甲苯噻嗪注射到腹腔内，用30 G针麻醉。上腹部和右胸部裁剪，去除头发和70％的乙醇，然后一个外用碘伏消毒液彻底清洗切口部位。 </li><li>动物插管endotracheally使用16 g静脉导管连接到小动物呼吸机（哈佛器械）。呼吸器是连接到一个蒸发器，提供0.5〜1.0％异氟醚（百特）100％的氧气混合，以维持全身麻醉。调整呼?...

<ol>
	<li>Choi, Y. H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Cardiac+conduction+through+engineered+tissue.">Cardiac conduction through engineered tissue.</a> <em style='font-style: italic'>Am J Pathol</em>. <b>169</b>, 72-85 (2006).</li><li>Shin, J. W. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Low-dose+systemic+bupivacaine+prevents+the+development+of+allodynia+after+thoracotomy+in+rats.+Anesth+Analg.">Low-dose systemic bupivacaine prevents the development of allodynia after thoracotomy in rats. Anesth Analg.</a> <b>107</b>, 1587-1591 (2008).</li><li>Horstick, G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Surgical+procedure+affects+physiological+parameters+in+rat+myocardial+ischemia:+need+for+mechanical+ventilation.">Surgical procedure affects physiological parameters in rat myocardial ischemia: need for mechanical ventilation.</a> <em style='font-style: italic'>Am J Physiol</em>. <b>276</b>, H472-H479 (1999).</li></ol>

<p class="jove_content">对于手术过程的一个有利的结果，关键是正确插管及侵入性的动物通风。如果老鼠是简单的镇静剂和允许自发呼吸，肺部都将不可避免地崩溃，而胸腔打开由于在啮齿类动物的共同胸膜。胸部闭幕前，必须完全消除任何气胸和肺部完全展开，以减少肺不张。否则，断奶呼吸器和恢复将是不成功的和动物将成为高碳酸血症和/或缺氧<sup>[3]。</sup>去除外膜组织内的AV槽是潜在的危险，并可能导致大量?...

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<td>7x4 Thermo Pad 4/box</td>
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<td>Hood Thermo-Pad, Summerland, BC Canada</td>
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<td>Inspira ASV Ventilator</td>
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<td>Handheld Pulse Oximeter</td>
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<td>Nellcor, Boulder, CO 80301 USA </td>
<td>N20PA</td>
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<td>Retractor</td>
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<td>Roboz, Gaithersburg, MD 20898 USA</td>
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<td>Bupivacaine 0.25%</td>
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<td>HOSPIRA Inc., Lake Forest, IL 60045</td>
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implantation of engineered tissue in the rat heart

<p class="jove_content">鼠类手术往往是在评估工程组织的效用的重要组成部分。一个可以进行多种外科手术中常见的实验室大鼠或小鼠，这些屡有作为台式实验和大量的动物试验或人体试验之间的一个中间步骤。由于啮齿类动物提供了建立，成本效益，和生理相关的模型系统测试新型脚手架材料和细胞组合，他们是特别适合心血管组织工程研究。目前，我们描述了一个开放的心脏外科手术植入工程组织含有生肌祖细胞在房室（AV）的老鼠心脏的凹​​槽。这些植入物的目的是建立完整的心脏传导阻滞[1]的儿科患者提供一种替代传统的心脏起搏器植入术治疗的最终目标与右心房和右心室之间的电气管道。工程的组织是通过开胸手术的方式植入在AV槽。对于我们而言，Lewis大鼠麻醉，侵入性通风，保持气道正压在无菌手术过程。向心的方法是通过安特罗外侧切口，由右侧开胸在5<sup&gt; TH</sup&gt;肋间。在AV槽，使用单一的7-0普理灵缝合的组织结构是固定的腹侧部分心脏的右心室和心房之间的定位。心外膜部分切除，让收件人，并在工程组织中的心肌细胞之间的直接接触。植入之后，胸壁层封闭，疏散，任何气胸和动物拔管和镇痛治疗。</p>

Watch this Scientific Journal Video about Implantation of Engineered Tissue in the Rat Heart at JoVE.com

Implantation of Engineered Tissue in the Rat Heart

<p class="jove_content">在这里，我们描述一个心脏外科手术植入房室沟成年Lewis大鼠的工程组织。</p>

大鼠心脏中植入的工程组织

Rodent surgery is often an important component in assessing the utility of engineered tissues. A wide variety of surgical procedures can be performed in ...

Research

JoVE Journal

Biology

Fabrication of Myogenic Engineered Tissue Constructs

制作生肌工程的组织结构

Despite the fact that electronic pacemakers are life-saving medical devices, their long-term performance in pediatric patients can be problematic owing to ...

科研

生物学

Primary Culture of Adult Rat Heart Myocytes

成年大鼠心脏心肌细胞的原代培养

Cultured primary adult rodent heart cells are an important model system for cardiovascular research.  Nevertheless, establishment of robust, viable ...

Visualizing the Beating Heart in <em>Drosophila</em>

Visualizing the Beating Heart in Drosophila

可视化心脏跳动<em>果蝇</em

The Drosophila heart has recently emerged as a good model system for examining the genetic, cellular, and molecular mechanisms underlying function in ...

Preparation of embryos for Electron Microscopy of the <em>Drosophila</em> embryonic heart tube

Preparation of embryos for Electron Microscopy of the Drosophila embryonic heart tube

电子显微镜对胚胎的制备<em>果蝇</em>胚胎心管

The morphogenesis of the Drosophila  embryonic heart tube has emerged as a valuable model system for studying cell migration, cell-cell adhesion and cell ...

Quantification of &gamma;H2AX Foci in Response to Ionising Radiation

Quantification of ÃƒÅ½Ã‚Â³H2AX Foci in Response to Ionising Radiation

电离辐射的反应定量的γH2AX灶

DNA double-strand breaks (DSBs), which are induced by either endogenous metabolic processes or by exogenous sources, are one of the most critical DNA ...

Quantitation of &gamma;H2AX Foci in Tissue Samples

Quantitation of ÃƒÅ½Ã‚Â³H2AX Foci in Tissue Samples

定量组织样本中的γH2AX灶

DNA double-strand breaks (DSBs) are particularly lethal and genotoxic lesions, that can arise either by endogenous (physiological or pathological) ...

Preparation of Highly Coupled Rat Heart Mitochondria

高度耦合的大鼠心肌线粒体的制备

The function of mitochondria in generation of cellular ATP in the process of oxidative phosphorylation is widely recognised. During the past decades there ...

Evaluation of the Spatial Distribution of &gamma;H2AX following Ionizing Radiation

Evaluation of the Spatial Distribution of ÃƒÅ½Ã‚Â³H2AX following Ionizing Radiation

评价以下电离辐射的空间分布的γH2AX

An early molecular response to DNA double-strand breaks (DSBs) is phosphorylation of the Ser-139 residue within the terminal SQEY motif of the histone ...

Clonogenic Assay: Adherent Cells

克隆形成含量：贴壁细胞

The clonogenic (or colony forming) assay has been established for more than 50 years; the original paper describing the technique was published in 19561. ...

Mouse Sperm Cryopreservation and Recovery using the I&middot;Cryo Kit

Mouse Sperm Cryopreservation and Recovery using the IÃƒâ€šÃ‚Â·Cryo Kit

精子冷冻保存和恢复使用I ·冷冻包

Thousands of new genetically modified (GM) strains of mice have been created since the advent of transgenesis and knockout technologies.  Many of these ...