Name: measuring caenorhabditis elegans life span on solid media
Uploaded: 2009-05-12T00:00:00
Description: Watch this Scientific Journal Video about Measuring Caenorhabditis elegans Life Span on Solid Media at JoVE.com

Dit werk werd ondersteund door een Glenn / verre Doorbraken in Gerontologie Award en NIH Grant 1R01AG031108-01 naar MKGS wordt ondersteund door NIH training te verlenen P30AG013280. MK is een Ellison Medical Foundation New Scholar in Aging.

Deel 1: Bereid nematode groei media (NGM) platen Deze paragraaf beschrijft de bereiding van het vaste NGM platen voor gebruik in de levensduur experiment. Een basis levensduur experiment zijn twee types van platen: standaard NGM platen, die geen additieven, en Amp / FUDR platen, die zowel ampicilline (Amp) en fluorodeoxyuridine (FUDR) toegevoegd aan de NGM hebben bevatten. Ampicilline wordt gebruikt om buitenlandse bacteriële besmetting te voorkomen. FUDR remt de celdeling, vermindert de prod...

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De genetische controle van de levensduur is uitgebreid onderzocht in C. elegans, grotendeels te danken aan het gemak en de snelheid waarmee levensduur kan worden vastgesteld. Het protocol besproken in dit artikel beschrijft een raamwerk voor het verkrijgen van reproduceerbare levensduur gegevens in C. elegans en kan ook worden toegepast op relatednematode soorten. 2 Door het maken van een aantal eenvoudige aanpassingen van deze procedures kunnen worden aangepast aan de levensduur te meten on...

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		<th>Material Name</th>
		<th>Type</th>
		<th>Company</th>
		<th>Catalogue Number</th>
		<th>Comment</th>
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<tr>
<td>Agar</td>
<td>Reagent</td>
<td>Fisher Scientific (BD Diagnostic Systems)</td>
<td>DF0145-17-0 (214530)</td>
<td>&nbsp;</td>
<tr>
<td>Ampicillin</td>
<td>Reagent</td>
<td>MidSci</td>
<td>0339</td>
<td>&nbsp;</td>
<tr>
<td>BactoTryptone</td>
<td>Reagent</td>
<td>Fisher Scientific (BD Diagnostic Systems)</td>
<td>DF0123-17-3 (211705)</td>
<td>&nbsp;</td>
<tr>
<td>BactoPeptone</td>
<td>Reagent</td>
<td>Fisher Scientific (BD Diagnostic Systems)</td>
<td>DF0118-17-0 (211677)</td>
<td>&nbsp;</td>
<tr>
<td>CaCl2</td>
<td>Reagent</td>
<td>Fisher Scientific (JT Baker)</td>
<td>NC9699248 (1332-01)</td>
<td>&nbsp;</td>
<tr>
<td>Carbenicillin</td>
<td>Reagent</td>
<td>Gold BioTechnology (GBT)</td>
<td>C0109</td>
<td>&nbsp;</td>
<tr>
<td>Cholesterol</td>
<td>Reagent</td>
<td>Sigma-Aldrich</td>
<td>C75209</td>
<td>&nbsp;</td>
<tr>
<td>Fluorodeoxyuridine (FUDR)</td>
<td>Reagent</td>
<td>Sigma-Aldrich</td>
<td>F0503</td>
<td>&nbsp;</td>
<tr>
<td>Isopropyl &#x03B2;-d-Thiogalactopyranoside (IPTG)</td>
<td>Reagent</td>
<td>Gold BioTechnology (GBT)</td>
<td>I2481C5</td>
<td>&nbsp;</td>
<tr>
<td>KPi Dibasic</td>
<td>Reagent</td>
<td>Fisher Scientific (JT Baker)</td>
<td>5087862 (3252-01)</td>
<td>&nbsp;</td>
<tr>
<td>KPi Monobasic</td>
<td>Reagent</td>
<td>Fisher Scientific (JT Baker)</td>
<td>5087861 (3246-01)</td>
<td>&nbsp;</td>
<tr>
<td>MgSO4</td>
<td>Reagent</td>
<td>Fisher Scientific (JT Baker)</td>
<td>NC9561800 (2500-01)</td>
<td>&nbsp;</td>
<tr>
<td>NaCl</td>
<td>Reagent</td>
<td>Fisher Scientific</td>
<td>S251</td>
<td>&nbsp;</td>
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<td>Tris</td>
<td>Reagent</td>
<td>Sigma-Aldrich</td>
<td>T1503</td>
<td>&nbsp;</td>
<tr>
<td>Yeast Extract</td>
<td>Reagent</td>
<td>Fisher Scientific (BD Diagnostic Systems)</td>
<td>DF0886-17-0 (288620)</td>
<td>&nbsp;</td>		</tbody>
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measuring caenorhabditis elegans life span on solid media

Veroudering is een degeneratieve proces wordt gekenmerkt door een progressieve verslechtering van de cellulaire componenten en organellen resulterend in sterfte. De nematode<em&gt; Caenorhabditis elegans</em&gt; Heeft zich ontpopt als een van de belangrijkste model dat wordt gebruikt om de biologie van veroudering te bestuderen. Omdat vrijwel alle biologische subsysteem ondergaat functionele achteruitgang met de leeftijd, de levensduur is het primaire eindpunt van belang bij het overwegen van totale tarief van de vergrijzing. In nematoden, is de levensduur meestal gedefinieerd als het aantal dagen dat een dierlijke resten reageren op prikkels van buitenaf. Aaltjes kan worden gepropageerd in vloeibare media of op vaste media in platen, en technieken zijn ontwikkeld voor het meten van de levensduur onder beide omstandigheden. Hier presenteren wij een algemeen protocol voor het meten van de levensduur van nematoden gehouden op vaste nematode groei media en gevoed met een dieet van UV-gedode bacteriën. Deze procedures kunnen eenvoudig worden aangepast aan de test levensduur onder verschillende voorkomende aandoeningen, waaronder een dieet bestaande uit levende bacteriën, calorische restrictie, en RNA-interferentie.

Watch this Scientific Journal Video about Measuring Caenorhabditis elegans Life Span on Solid Media at JoVE.com

Measuring Caenorhabditis elegans Life Span on Solid Media

In dit artikel geven we een algemeen protocol voor het meten van de levensduur van nematoden gehouden op vaste media met UV-gedode bacteriën voedsel.

Het meten van Caenorhabditis elegans Levensduur over Solid Media

Aging is a degenerative process characterized by a progressive deterioration of cellular components and organelles resulting in mortality. The nematode ...

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Measuring Caenorhabditis elegans Life Span on Solid Media

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The budding yeast Saccharomyces cerevisiae has proven to be an important model organism in the field of aging research 1. The replicative and ...

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Aging is a degenerative process characterized by a progressive deterioration of cellular components and organelles resulting in mortality. The budding ...

Measuring Caenorhabditis elegans Life Span in 96 Well Microtiter Plates

Measuring Caenorhabditis elegans Life Span in 96 Well Microtiter Plates

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Lifespan is a biological process regulated by several genetic pathways. One strategy to investigate the biology of aging is to study animals that harbor ...

Solid Plate-based Dietary Restriction in Caenorhabditis elegans

Solid Plate-based Dietary Restriction in Caenorhabditis elegans

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Reduction of food intake without malnutrition or starvation is known to increase lifespan and delay the onset of various age-related diseases in a wide ...

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Kweken<em&gt; Caenorhabditis elegans</em &gt; In Axenische Liquid Media en Creatie van transgene Worms door bombardement met microdeeltjes

In this protocol, we present the required materials, and the procedure for making modified&#160;C. elegans&#160;Habituation and Reproduction media ...

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Measuring Oxidative Stress Resistance of Caenorhabditis elegans in 96-well Microtiter Plates

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Oxidative stress, which is the result of an imbalance between production and detoxification of reactive oxygen species, is a major contributor to chronic ...

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Correct folding and assembly of proteins and protein complexes are essential for cellular function. Cells employ quality control pathways that correct, ...

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Labelvrije beeldvorming van lipideopslagdynamiek bij Caenorhabditis elegans met behulp van gestimuleerde Raman-verstrooiingscopie

Lipid metabolism is a fundamental physiological process necessary for cellular and organism health. Dysregulation of lipid metabolism often elicits ...

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A rise in the prevalence of neurodegenerative protein conformational diseases (PCDs) has fostered a great interest in this subject over the years. This ...