Name: measuring caenorhabditis elegans life span on solid media
Uploaded: 2009-05-12T00:00:00
Description: Watch this Scientific Journal Video about Measuring Caenorhabditis elegans Life Span on Solid Media at JoVE.com

Ce travail a été soutenu par une Percées Glenn / Afar en gérontologie et le Prix des subventions du NIH 1R01AG031108-01 à MKGS est soutenu par le NIH bourse de formation P30AG013280. MK est une Ellison Medical Foundation New Scholar liés au vieillissement.

Partie 1: Préparer la croissance des médias nématodes (NGM) plaques Cette section décrit la préparation des plaques NGM solide pour une utilisation dans l&#39;expérience la durée de vie. Une expérience de vie de base durée nécessite deux types de plaques: les plaques NGM standard, qui ne contiennent pas d&#39;additifs, et Amp / FUDR plaques, qui ont à la fois à l&#39;ampicilline (Amp) et fluorodésoxyuridine (FUDR) ajouté à l&#39;assemblée générale nationale. L&#39;ampicilline...

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Le contrôle génétique de la longévité a été largement étudié en C. elegans, largement dû à la facilité et la rapidité avec laquelle la durée de vie peut être déterminée. Le protocole abordées dans cet article décrit un cadre de base pour obtenir des données reproductibles durée de vie en C. elegans et peut également être appliquée à des espèces relatednematode. 2 En faisant quelques simples modifications de ces procédures peuvent être adaptés pour mesurer la dur?...

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		<th>Catalogue Number</th>
		<th>Comment</th>
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<td>Agar</td>
<td>Reagent</td>
<td>Fisher Scientific (BD Diagnostic Systems)</td>
<td>DF0145-17-0 (214530)</td>
<td>&nbsp;</td>
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<td>Ampicillin</td>
<td>Reagent</td>
<td>MidSci</td>
<td>0339</td>
<td>&nbsp;</td>
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<td>BactoTryptone</td>
<td>Reagent</td>
<td>Fisher Scientific (BD Diagnostic Systems)</td>
<td>DF0123-17-3 (211705)</td>
<td>&nbsp;</td>
<tr>
<td>BactoPeptone</td>
<td>Reagent</td>
<td>Fisher Scientific (BD Diagnostic Systems)</td>
<td>DF0118-17-0 (211677)</td>
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<td>CaCl2</td>
<td>Reagent</td>
<td>Fisher Scientific (JT Baker)</td>
<td>NC9699248 (1332-01)</td>
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<td>Reagent</td>
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<td>Reagent</td>
<td>Sigma-Aldrich</td>
<td>C75209</td>
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<td>Reagent</td>
<td>Sigma-Aldrich</td>
<td>F0503</td>
<td>&nbsp;</td>
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<td>Isopropyl &#x03B2;-d-Thiogalactopyranoside (IPTG)</td>
<td>Reagent</td>
<td>Gold BioTechnology (GBT)</td>
<td>I2481C5</td>
<td>&nbsp;</td>
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<td>Reagent</td>
<td>Fisher Scientific (JT Baker)</td>
<td>5087862 (3252-01)</td>
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<td>Reagent</td>
<td>Fisher Scientific (JT Baker)</td>
<td>5087861 (3246-01)</td>
<td>&nbsp;</td>
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<td>MgSO4</td>
<td>Reagent</td>
<td>Fisher Scientific (JT Baker)</td>
<td>NC9561800 (2500-01)</td>
<td>&nbsp;</td>
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<td>Reagent</td>
<td>Fisher Scientific</td>
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<td>Reagent</td>
<td>Sigma-Aldrich</td>
<td>T1503</td>
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<td>Yeast Extract</td>
<td>Reagent</td>
<td>Fisher Scientific (BD Diagnostic Systems)</td>
<td>DF0886-17-0 (288620)</td>
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measuring caenorhabditis elegans life span on solid media

Le vieillissement est un processus dégénératif caractérisé par une détérioration progressive des composants cellulaires et des organites résultant de la mortalité. Le nématode<em&gt; Caenorhabditis elegans</em&gt; A émergé comme un modèle principal utilisé pour étudier la biologie du vieillissement. Parce que pratiquement tous les sous-systèmes biologiques subit un déclin fonctionnel avec l&#39;âge, la durée de vie est le critère principal d&#39;intérêt lorsque l&#39;on considère le taux total de vieillissement. Dans les nématodes, la durée de vie est généralement défini comme le nombre de jours d&#39;un animal reste sensible aux stimuli extérieurs. Les nématodes peuvent être propagés soit dans des milieux liquides ou sur des supports solides en plaques, et les techniques ont été développées pour mesurer la durée de vie dans les deux conditions. Nous présentons ici un protocole généralisé pour mesurer la durée de vie des nématodes maintenue sur milieu solide croissance des nématodes et nourris avec un régime d&#39;UV-tué les bactéries. Ces procédures peuvent être facilement adaptés à la durée de vie de dosage dans différentes conditions communes, y compris un régime alimentaire composé de bactéries vivantes, des restrictions alimentaires, et l&#39;interférence ARN.

Watch this Scientific Journal Video about Measuring Caenorhabditis elegans Life Span on Solid Media at JoVE.com

Measuring Caenorhabditis elegans Life Span on Solid Media

Dans cet article nous présentons un protocole général pour la mesure de la durée de vie des nématodes maintenu sur un support solide avec UV-tué alimentaire bactérienne.

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