Name: measuring caenorhabditis elegans life span on solid media
Uploaded: 2009-05-12T00:00:00
Description: Watch this Scientific Journal Video about Measuring Caenorhabditis elegans Life Span on Solid Media at JoVE.com

Este trabalho foi financiado por um Avanços Glenn / AFAR em Gerontologia e Grant Award NIH 1R01AG031108-01 a MKGS é suportado pelo NIH treinamento conceder P30AG013280. MK é um Ellison Medical Foundation Scholar novo no Envelhecimento.

Parte 1: Prepare nematóide crescimento media (NGM) placas Esta seção descreve a preparação das placas NGM sólido para uso no experimento vida. A experiência de extensão básicas da vida requer dois tipos de placas: placas NGM padrão, que não contêm aditivos, e Amp / FUDR placas, que têm ampicilina (Amp) e fluorodeoxyuridine (FUDR) adicionado ao NGM. Ampicilina é usado para prevenir a contaminação bacteriana estrangeiros. FUDR inibe a divisão celular, reduz a produção de ovos,...

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O controle genético da longevidade tem sido estudado extensivamente em C. elegans, em grande parte devido à facilidade e rapidez com que expectativa de vida pode ser determinada. O protocolo discutido neste artigo descreve uma estrutura básica para a obtenção de dados de vida reprodutíveis extensão em C. elegans e também pode ser aplicado às espécies relatednematode. 2 Ao fazer algumas alterações simples desses procedimentos pode ser adaptado para medir a expectativa de vida sob ...

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		<th>Catalogue Number</th>
		<th>Comment</th>
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<td>Agar</td>
<td>Reagent</td>
<td>Fisher Scientific (BD Diagnostic Systems)</td>
<td>DF0145-17-0 (214530)</td>
<td>&nbsp;</td>
<tr>
<td>Ampicillin</td>
<td>Reagent</td>
<td>MidSci</td>
<td>0339</td>
<td>&nbsp;</td>
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<td>BactoTryptone</td>
<td>Reagent</td>
<td>Fisher Scientific (BD Diagnostic Systems)</td>
<td>DF0123-17-3 (211705)</td>
<td>&nbsp;</td>
<tr>
<td>BactoPeptone</td>
<td>Reagent</td>
<td>Fisher Scientific (BD Diagnostic Systems)</td>
<td>DF0118-17-0 (211677)</td>
<td>&nbsp;</td>
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<td>CaCl2</td>
<td>Reagent</td>
<td>Fisher Scientific (JT Baker)</td>
<td>NC9699248 (1332-01)</td>
<td>&nbsp;</td>
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<td>Reagent</td>
<td>Gold BioTechnology (GBT)</td>
<td>C0109</td>
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<td>Reagent</td>
<td>Sigma-Aldrich</td>
<td>C75209</td>
<td>&nbsp;</td>
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<td>Reagent</td>
<td>Sigma-Aldrich</td>
<td>F0503</td>
<td>&nbsp;</td>
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<td>Isopropyl &#x03B2;-d-Thiogalactopyranoside (IPTG)</td>
<td>Reagent</td>
<td>Gold BioTechnology (GBT)</td>
<td>I2481C5</td>
<td>&nbsp;</td>
<tr>
<td>KPi Dibasic</td>
<td>Reagent</td>
<td>Fisher Scientific (JT Baker)</td>
<td>5087862 (3252-01)</td>
<td>&nbsp;</td>
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<td>KPi Monobasic</td>
<td>Reagent</td>
<td>Fisher Scientific (JT Baker)</td>
<td>5087861 (3246-01)</td>
<td>&nbsp;</td>
<tr>
<td>MgSO4</td>
<td>Reagent</td>
<td>Fisher Scientific (JT Baker)</td>
<td>NC9561800 (2500-01)</td>
<td>&nbsp;</td>
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<td>NaCl</td>
<td>Reagent</td>
<td>Fisher Scientific</td>
<td>S251</td>
<td>&nbsp;</td>
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<td>Tris</td>
<td>Reagent</td>
<td>Sigma-Aldrich</td>
<td>T1503</td>
<td>&nbsp;</td>
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<td>Yeast Extract</td>
<td>Reagent</td>
<td>Fisher Scientific (BD Diagnostic Systems)</td>
<td>DF0886-17-0 (288620)</td>
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measuring caenorhabditis elegans life span on solid media

O envelhecimento é um processo degenerativo caracterizado por uma deterioração progressiva de componentes celulares e organelas, resultando em mortalidade. O nematóide<em&gt; Caenorhabditis elegans</em&gt; Surgiu como um modelo principal utilizado para estudar a biologia do envelhecimento. Porque praticamente todos os subsistemas biológico sofre declínio funcional com o aumento da idade, expectativa de vida é o objetivo primário de interesse quando se considera taxa total de envelhecimento. Em nematóides, expectativa de vida é normalmente definida como o número de dias de um animal continua a responder a estímulos externos. Nematóides podem ser propagadas tanto em meios líquidos ou em meios sólidos em placas, e as técnicas foram desenvolvidas para medir o tempo de vida em ambas as condições. Aqui apresentamos um protocolo generalizada para medir tempo de vida dos nematóides mantidos em meio de crescimento sólido de nematóides e alimentados com uma dieta de UV-morto bactérias. Estes procedimentos podem ser facilmente adaptado a vida útil do ensaio sob várias condições comuns, incluindo uma dieta composta de bactérias vivas, a restrição alimentar e interferência de RNA.

Watch this Scientific Journal Video about Measuring Caenorhabditis elegans Life Span on Solid Media at JoVE.com

Measuring Caenorhabditis elegans Life Span on Solid Media

Neste artigo apresentamos um protocolo geral para medir a expectativa de vida dos nematóides mantidos em meios sólidos com alimentos UV-morto bacteriana.

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