Name: measuring caenorhabditis elegans life span on solid media
Uploaded: 2009-05-12T00:00:00
Description: Watch this Scientific Journal Video about Measuring Caenorhabditis elegans Life Span on Solid Media at JoVE.com

Este trabajo fue apoyado por una Glenn / AFAR avances en la concesión de Gerontología y Subvenciones del NIH 1R01AG031108-01 a MKGS es apoyado por el NIH formación conceder P30AG013280. MK es una Ellison Medical Foundation de Nueva Académico en el envejecimiento.

Parte 1: Preparar nematodo crecimiento de los medios (NGM) placas En esta sección se describe la preparación de los platos NGM sólida para su uso en el experimento de vida. Un experimento de vida útil básica requiere dos tipos de placas: las placas estándar NGM, que no contienen aditivos, y las placas Amp / FUDR, que tienen la ampicilina (Amp) y fluorodesoxiuridina (FUDR) añadido a la NGM. La ampicilina se utiliza para prevenir la contaminación bacteriana externa. FUDR inhibe la divisi...

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El control genético de la longevidad se ha estudiado extensamente en C. elegans, en gran parte debido a la facilidad y rapidez con que puede ser tiempo de vida determinado. El protocolo discutido en este artículo se describe un marco básico para la obtención de datos reproducibles esperanza de vida en C. elegans y también se puede aplicar a las especies relatednematode. 2 Al hacer algunos cambios sencillos estos procedimientos pueden ser adaptados para medir duración de la vida bajo un...

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<td>Agar</td>
<td>Reagent</td>
<td>Fisher Scientific (BD Diagnostic Systems)</td>
<td>DF0145-17-0 (214530)</td>
<td>&nbsp;</td>
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<td>Ampicillin</td>
<td>Reagent</td>
<td>MidSci</td>
<td>0339</td>
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<td>BactoTryptone</td>
<td>Reagent</td>
<td>Fisher Scientific (BD Diagnostic Systems)</td>
<td>DF0123-17-3 (211705)</td>
<td>&nbsp;</td>
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<td>Reagent</td>
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<td>F0503</td>
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<td>Reagent</td>
<td>Gold BioTechnology (GBT)</td>
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<td>Reagent</td>
<td>Fisher Scientific (JT Baker)</td>
<td>5087862 (3252-01)</td>
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<td>Fisher Scientific (JT Baker)</td>
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<td>MgSO4</td>
<td>Reagent</td>
<td>Fisher Scientific (JT Baker)</td>
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<td>Reagent</td>
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<td>Reagent</td>
<td>Fisher Scientific (BD Diagnostic Systems)</td>
<td>DF0886-17-0 (288620)</td>
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measuring caenorhabditis elegans life span on solid media

El envejecimiento es un proceso degenerativo caracterizado por un deterioro progresivo de los componentes celulares y orgánulos que resulta en la mortalidad. El nematodo<em&gt; Caenorhabditis elegans</em&gt; Se ha convertido en un modelo principal utilizado para estudiar la biología del envejecimiento. Debido a que prácticamente todos los subsistemas biológico sufre deterioro funcional que aumenta la edad, duración de la vida es el objetivo primario de interés cuando se considera la tasa total de envejecimiento. En los nematodos, esperanza de vida se define normalmente como el número de días que un animal sigue siendo sensible a los estímulos externos. Los nematodos pueden propagarse, ya sea en medios líquidos o en el medio sólido en placas, y se han desarrollado técnicas para medir la duración de la vida en ambas condiciones. Aquí se presenta un protocolo generalizado para medir la duración de la vida de los nematodos mantienen en medios sólidos nematodo crecimiento y alimentados con una dieta de muertos UV-bacterias. Estos procedimientos se pueden adaptar fácilmente para abarcar ensayo de la vida bajo diversas condiciones comunes, incluyendo una dieta a base de bacterias vivas, la restricción dietética, y la interferencia de ARN.

Watch this Scientific Journal Video about Measuring Caenorhabditis elegans Life Span on Solid Media at JoVE.com

Measuring Caenorhabditis elegans Life Span on Solid Media

En este artículo se presenta un protocolo general para medir la duración de la vida de los nematodos mantienen en medios sólidos con UV-muertos alimentaria bacteriana.

Medición Caenorhabditis elegans Duración de la vida en el medio sólido

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The budding yeast Saccharomyces cerevisiae has proven to be an important model organism in the field of aging research 1. The replicative and ...

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Solid Plate-based Dietary Restriction in Caenorhabditis elegans

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In this protocol, we present the required materials, and the procedure for making modified&#160;C. elegans&#160;Habituation and Reproduction media ...

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Lipid metabolism is a fundamental physiological process necessary for cellular and organism health. Dysregulation of lipid metabolism often elicits ...

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A rise in the prevalence of neurodegenerative protein conformational diseases (PCDs) has fostered a great interest in this subject over the years. This ...