Name: measuring caenorhabditis elegans life span on solid media
Uploaded: 2009-05-12T00:00:00
Description: Watch this Scientific Journal Video about Measuring Caenorhabditis elegans Life Span on Solid Media at JoVE.com

Detta arbete stöddes av en Glenn / FJÄRRAN Genombrott i gerontologi Award och NIH Grant 1R01AG031108-01 till MKGS stöds av NIH utbildningsbidrag P30AG013280. MK är en Ellison Medical Foundation Nya Scholar i åldrande.

Del 1: Förbered rundmasken tillväxt media (NGM) plattor Detta avsnitt beskriver förberedelserna av det fasta NGM plattorna för användning i livslängd experimentet. En grundläggande livslängd experiment kräver två typer av plattor: Standard NGM plattor, som innehåller inga tillsatser, och AMP / FUDR plattor, som har både ampicillin (Amp) och fluorodeoxyuridine (FUDR) läggs till NGM. Ampicillin används för att förhindra utländska bakteriell kontamination. FUDR hämmar celldelnin...

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Den genetiska kontrollen av livslängden har studerats utförligt i C. elegans, mycket tack vare den lätthet och snabbhet med vilken livslängd kan fastställas. Protokollet diskuteras i denna artikel beskriver en grundläggande ram för att få reproducerbara uppgifter livslängd i C. elegans och kan även tillämpas på relatednematode arter. Genom att göra några enkla förändringar dessa förfaranden kan anpassas för att mäta livslängd under olika villkor 2. Här kommer vi att disk...

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		<th>Catalogue Number</th>
		<th>Comment</th>
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<td>Agar</td>
<td>Reagent</td>
<td>Fisher Scientific (BD Diagnostic Systems)</td>
<td>DF0145-17-0 (214530)</td>
<td>&nbsp;</td>
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<td>Ampicillin</td>
<td>Reagent</td>
<td>MidSci</td>
<td>0339</td>
<td>&nbsp;</td>
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<td>BactoTryptone</td>
<td>Reagent</td>
<td>Fisher Scientific (BD Diagnostic Systems)</td>
<td>DF0123-17-3 (211705)</td>
<td>&nbsp;</td>
<tr>
<td>BactoPeptone</td>
<td>Reagent</td>
<td>Fisher Scientific (BD Diagnostic Systems)</td>
<td>DF0118-17-0 (211677)</td>
<td>&nbsp;</td>
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<td>CaCl2</td>
<td>Reagent</td>
<td>Fisher Scientific (JT Baker)</td>
<td>NC9699248 (1332-01)</td>
<td>&nbsp;</td>
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<td>Reagent</td>
<td>Gold BioTechnology (GBT)</td>
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<td>Reagent</td>
<td>Sigma-Aldrich</td>
<td>C75209</td>
<td>&nbsp;</td>
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<td>Reagent</td>
<td>Sigma-Aldrich</td>
<td>F0503</td>
<td>&nbsp;</td>
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<td>Isopropyl &#x03B2;-d-Thiogalactopyranoside (IPTG)</td>
<td>Reagent</td>
<td>Gold BioTechnology (GBT)</td>
<td>I2481C5</td>
<td>&nbsp;</td>
<tr>
<td>KPi Dibasic</td>
<td>Reagent</td>
<td>Fisher Scientific (JT Baker)</td>
<td>5087862 (3252-01)</td>
<td>&nbsp;</td>
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<td>KPi Monobasic</td>
<td>Reagent</td>
<td>Fisher Scientific (JT Baker)</td>
<td>5087861 (3246-01)</td>
<td>&nbsp;</td>
<tr>
<td>MgSO4</td>
<td>Reagent</td>
<td>Fisher Scientific (JT Baker)</td>
<td>NC9561800 (2500-01)</td>
<td>&nbsp;</td>
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<td>NaCl</td>
<td>Reagent</td>
<td>Fisher Scientific</td>
<td>S251</td>
<td>&nbsp;</td>
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<td>Tris</td>
<td>Reagent</td>
<td>Sigma-Aldrich</td>
<td>T1503</td>
<td>&nbsp;</td>
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<td>Yeast Extract</td>
<td>Reagent</td>
<td>Fisher Scientific (BD Diagnostic Systems)</td>
<td>DF0886-17-0 (288620)</td>
<td>&nbsp;</td>		</tbody>
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measuring caenorhabditis elegans life span on solid media

Åldrande är en degenerativ process som kännetecknas av en progressiv försämring av cellulära komponenter och organeller resulterade i dödlighet. Nematoden<em&gt; Caenorhabditis elegans</em&gt; Har vuxit fram som en huvudman modell som används för att studera åldrandets biologi. Eftersom nästan alla biologiska delsystem genomgår funktionell avtar med stigande ålder, är livslängden det primära effektmåttet av intresse när man överväger totalnivå för åldrande. I nematoder, är livslängden vanligtvis definieras som det antal dagar ett djur är mottaglig för yttre stimuli. Nematoder kan förökas antingen i flytande media eller på fasta medier i form av plattor, och tekniker har utvecklats för att mäta livslängd under båda förhållanden. Här presenterar vi en generell protokoll för mätning av livslängden på nematoder kvar på fasta medier nematod tillväxt och utfodras med en diet av UV-dödade bakterier. Dessa förfaranden kan enkelt anpassas till analysen livslängd under olika gemensamma villkor, bland annat en diet bestående av levande bakterier, dietrestriktioner och RNA-interferens.

Watch this Scientific Journal Video about Measuring Caenorhabditis elegans Life Span on Solid Media at JoVE.com

Measuring Caenorhabditis elegans Life Span on Solid Media

I denna artikel presenterar vi en allmän protokoll för mätning av livslängden på nematoder kvar på fasta medier med UV dödade-bakteriell mat.

Mätning Caenorhabditis elegans Livslängd på fasta medier

Aging is a degenerative process characterized by a progressive deterioration of cellular components and organelles resulting in mortality. The nematode ...

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Measuring Caenorhabditis elegans Life Span on Solid Media

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The budding yeast Saccharomyces cerevisiae has proven to be an important model organism in the field of aging research 1. The replicative and ...

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Measuring Caenorhabditis elegans Life Span in 96 Well Microtiter Plates

Measuring Caenorhabditis elegans Life Span in 96 Well Microtiter Plates

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Solid Plate-based Dietary Restriction in Caenorhabditis elegans

Solid Plate-based Dietary Restriction in Caenorhabditis elegans

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Reduction of food intake without malnutrition or starvation is known to increase lifespan and delay the onset of various age-related diseases in a wide ...

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Basic Caenorhabditis elegans Methods: Synchronization and Observation

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In this protocol, we present the required materials, and the procedure for making modified&#160;C. elegans&#160;Habituation and Reproduction media ...

Measuring Oxidative Stress Resistance of Caenorhabditis elegans in 96-well Microtiter Plates

Measuring Oxidative Stress Resistance of Caenorhabditis elegans in 96-well Microtiter Plates

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Oxidative stress, which is the result of an imbalance between production and detoxification of reactive oxygen species, is a major contributor to chronic ...

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Using Caenorhabditis elegans to Screen for Tissue-Specific Chaperone Interactions

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Correct folding and assembly of proteins and protein complexes are essential for cellular function. Cells employ quality control pathways that correct, ...

Label-Free Imaging of Lipid Storage Dynamics in Caenorhabditis elegans using Stimulated Raman Scattering Microscopy

Label-Free Imaging of Lipid Storage Dynamics in Caenorhabditis elegans using Stimulated Raman Scattering Microscopy

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Lipid metabolism is a fundamental physiological process necessary for cellular and organism health. Dysregulation of lipid metabolism often elicits ...

Automating Aggregate Quantification in Caenorhabditis elegans

Automating Aggregate Quantification in Caenorhabditis elegans

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A rise in the prevalence of neurodegenerative protein conformational diseases (PCDs) has fostered a great interest in this subject over the years. This ...