Name: nuclear transfer into mouse oocytes
Uploaded: 2006-11-30T00:00:00
Description: Watch this Scientific Journal Video about Nuclear Transfer into Mouse Oocytes at JoVE.com

DE想感谢他NT技巧和斯蒂芬沙利文博士和加勒特伯克霍夫共享协议的关键读博士隐藏阿久津。

准备工作： <ol><li class="jove_step">一个超数排卵和胚胎培养microdrop的详细描述，可以发现其他地方的2。 </li><li class="jove_step">准备好与胚胎培养基（如KSOM，Chemicon公司）microdrops培养皿。盖与矿物油（矿物油批次应与胚胎培养的兼容性测试）。平衡在37℃空气中加5％的CO 2中的C。 （热电子3110水套式孵化器或同等） </li><li class="jove_step">卵母细胞的隔离准备一道菜：HEPES广场滴缓冲CZB和...

<ol>
	<li>Eggan, K., Jaenisch, R., Pease, S., Lois, C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=.">.</a> Mammalian and Avian Transgenesis- New Approaches. , (2006).</li><li>Nagy, A., Gertsenstein, M., Vintersten, K., Behringer, R., Press, C. S. H. L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=.">.</a> Manipulating the mouse embryo. , (2003).</li><li>Wakayama, T., Perry, A. C., Zuccotti, M., Johnson, K. R., Yanagimachi, R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Full-term+development+of+mice+from+enucleated+oocytes+injected+with+cumulus+cell+nuclei.">Full-term development of mice from enucleated oocytes injected with cumulus cell nuclei.</a> Nature. 394, 369-374 (1998).</li></ol>

请检查是否有额外的缓冲区，在协议的一部分组成。

nuclear transfer into mouse oocytes

核移植到未受精的卵母细胞可以分化的细胞恢复发展潜力。这表明，底层的发育，分化和老化的过程，而不是遗传过程的后生。这些过程的可逆性打开令人兴奋的观点，在基础研究和在更遥远的未来，在再生医学。在小鼠胚胎干细胞可以从克隆胚胎植入前胚胎。这种胚胎干细胞会引起成人的有机体的所有细胞类型的能力。更重要的是，这些细胞是基因完全相同的捐助。如果适用于人类，这将允许从病人的干细胞的推导。然后将这些细胞可以分化成病人受影响的细胞类型，并研究在体外，或用来替换损坏或丢失的细胞。在小鼠核移植的研究仍然是重要的，因为它可以告知我们有关核重编程的原则。这部电影和所附议定书的目的是帮助学习在小鼠核移植，这种方法最初教授柳町（和歌山等，1998）集团开发的。

Watch this Scientific Journal Video about Nuclear Transfer into Mouse Oocytes at JoVE.com

Nuclear Transfer into Mouse Oocytes

这部电影的协议，旨在帮助学习核转移。

小鼠卵母细胞的细胞核移植到

Nuclear transfer into an unfertilized oocyte can restore developmental potential to a differentiated cell. This demonstrates that the processes underlying ...

Research

JoVE Journal

Biology

Retrieval of Mouse Oocytes

检索小鼠卵母细胞

To date, only a few studies have reported successful manipulations of Peromyscus embryogenesis or reproductive biology.  Together with the Peromyscus ...

科研

生物学

Microinjection of Xenopus Laevis Oocytes

非洲爪蟾卵母细胞的微量注射

Microinjection of Xenopus laevis oocytes followed by thin-sectioning electron microscopy (EM) is an excellent system for studying nucleocytoplasmic ...

Collection and Cryopreservation of Hamster Oocytes and Mouse Embryos

收集和仓鼠卵母细胞和小鼠胚胎冷冻保存

Embryos and oocytes were first successfully cryopreserved more than 30 years ago, when Whittingham et al. 1 and Wilmut 2 separately described that mouse ...

Fertilization of Xenopus oocytes using the Host Transfer Method

Fertilization of Xenopus oocytes using the Host Transfer Method

施肥爪蟾卵母细胞使用主机传输方法

Studying the contribution of maternally inherited molecules to vertebrate early development is often hampered by the time and expense necessary to ...

Direct Tracheal Instillation of Solutes into Mouse Lung

溶质直接气管滴入小鼠肺

Intratracheal instillations deliver solutes directly into the lungs.  This procedure targets the delivery of the instillate into the distal regions of the ...

In vivo Imaging and Therapeutic Treatments in an Orthotopic Mouse Model of Ovarian Cancer

In vivo Imaging and Therapeutic Treatments in an Orthotopic Mouse Model of Ovarian Cancer

活体成像和治疗卵巢癌的原位小鼠模型

Human cancer and response to therapy is better represented in orthotopic animal models.  This paper describes the development of an orthotopic mouse model ...

Gene Transfer into Older Chicken Embryos by ex ovo Electroporation

Gene Transfer into Older Chicken Embryos by ex ovo Electroporation

基因转移到老年人的鸡胚<em&gt;前OVO</em&gt;电穿孔

The chicken embryo provides an  excellent model system for studying gene function and regulation during  embryonic development. In ovo electroporation is ...

Utero-tubal Embryo Transfer and Vasectomy in the Mouse Model

子宫 - 输卵管胚胎移植和输精管结扎术在小鼠模型

The transfer of preimplantation embryos to a surrogate female is a required step for the production of genetically modified mice or to study the effects ...

Measuring DNA Damage and Repair in Mouse Splenocytes After Chronic In Vivo Exposure to Very Low Doses of Beta- and Gamma-Radiation

Measuring DNA Damage and Repair in Mouse Splenocytes After Chronic In Vivo Exposure to Very Low Doses of Beta- and Gamma-Radiation

测量DNA损伤与修复小鼠脾细胞慢性后<em&gt;在体内</em&gt;暴露于β和γ辐射剂量很低

Low dose radiation exposure may produce a variety of biological effects that are different in quantity and quality from the effects produced by high ...

Meiotic Spindle Assessment in Mouse Oocytes by siRNA-mediated Silencing

减数分裂纺锤体评估在小鼠卵母细胞的siRNA介导沉默

Errors in chromosome segregation during meiotic division in gametes can lead to aneuploidy that is subsequently transmitted to the embryo upon ...